摘要：Taxol is a 'blockbuster' antitumor drug produced by Taxus species with extremely low amount, while its analogue 7-β-xylosyl-10-deacetyltaxol is generally much higher in the plants. Both the fungal enzymes LXYL-P1à1 and LXYL-P1à2 can convert 7-β-xylosyl-10-deacetyltaxol into10-deacetyltaxol for Taxol semi-synthesis. Of them, LXYL-P1à2 is twice more active than LXYLP1à1, but there are only 11 significantly different amino acids in terms of the polarity and acidic-basic properties between them. In this study, single and multiple site-directed mutations at the 11 sites from LXYL-P1à1 to LXYL-P1à2 were performed to define the amino acids with upward bias in activities and to acquire variants with improved catalytic properties. Among all the 17 mutants, E12(A72 T/V91 S) was the most active and even displayed 2.8-and 3-fold higher than LXYL-P1à2 on β-xylosidase andβ-glucosidase activities. The possible mechanism for such improvement was proposed by homology modeling and molecular docking between E12 and 7-β-xylosyl-10-deacetyltaxol. The recombinant yeast GS115-P1 E12-7 was constructed by introducing variant E12, the molecular chaperone gene pdi and the bacterial hemoglobin gene vhb. This engineered yeast rendered 4 times higher biomass enzyme activity than GS115-3.5 K-P1à2 that had been used for demo-scale fermentation. Thus, GS115-P1 E12-7 becomes a promising candidate to replace GS115-3.5 K-P1à2 for industrial purpose.

摘要：目的对我国护理管理者在职培训需求进行分析。方法计算机检索中国知网、万方、维普、PubMed和Web of Science数据库,检索时限从2005年8月1日至2020年2月29日,检索我国护理管理者培训需求相关横断面调查,对护理管理者培训方式、培训时长、培训内容和培训师资方面的需求进行范围综述。结果共纳入23篇文献,包含21 495名研究对象。结果显示护理管理者最接受的培训方式是临床实地教学与跟班、培训班授课、案例讨论、专题讲座与研讨;培训时长一周左右最佳;师资选择主要为国内外一线的护理管理专家;在培训内容上,主要从护理管理、人力资源管理、财务管理、法律法规四个模块进行体系设计,其中护理质量、护理安全、绩效管理、团队建设和人际沟通等主题培训需求最大。结论建议根据需求设计护理管理人才培训内容和培训方式,以助于更有效地开展高质量培训。

摘要：With the continuous evolution of electronic technology,field-programmable gate array(FPGA)has demonstrated significant advantages in the realm of signal acquisition and processing,and signal acquisition plays a pivotal role in the practical applications of laser *** analysis of the output signals from a laser gyro and an accelerometer,this paper presents a circuit design for signal acquisition of the laser gyro based on domestic *** design incorporates a finite impulse response(FIR)filter to process the gyro signal and employs a small-volume,impact-resistant quartz flexible accelerometer for signal *** results demonstrate that the errors in X,Y,and Z axes fall within acceptable ranges while meeting filtering *** use of FPGA for signal acquisition and preprocessing enhances configuration flexibility,which provides an idea and method for optimizing performance and processing signals in laser gyro applications.

摘要：以含半乳糖的甲基丙烯酸羟乙酯和对苯乙烯磺酸钠为单体,通过可逆加成断裂链转移聚合设计合成了一系列具有不同结构的含糖高分子.采用核磁共振氢谱和凝胶渗透色谱对材料的结构进行了表征.分别通过比浊法和酶联免疫吸附实验,以花生凝集素(PNA)为模型,研究了磺酸基的数量及分布对材料与凝集素之间相互作用的影响.结果表明,磺酸基的引入能通过静电相互作用协同增强含半乳糖高分子与PNA之间的特异性结合作用,并且这一作用可通过磺酸基数量与排列方式进行调节,相对于嵌段共聚型材料,无规共聚型材料与PNA的结合作用更强,其中P(Gal21-r-SS41)与PNA的结合作用最强,较均聚半乳糖高分子(PGal)提高2.7倍.利用这种相互作用,材料能够通过阻断凝集素活性有效抑制肿瘤细胞的迁移,有望在临床抗肿瘤转移治疗中得以应用.

摘要：We report a novel benzoxazole derivative,1,4-bis(benzo[d]oxazol-2-yl)naphthalene(BBON),exhibiting exceptional multifunctional properties for advanced optoelectronic *** crystals demonstrate remarkable multidirectional bending and twisting at room temperature and retain elasticity under extreme conditions,such as exposure to liquid nitrogen,showcasing their *** crystals can be crafted into complex mesh and lantern shapes,highlighting their versatility for flexible and wearable *** high pressure,BBON exhibits significant piezochromic shifts,with the emission wavelength shifting from 477 to 545 nm upon pressure *** crystals,with a high quantum yield of 72.26%,exhibit excellent optical waveguide performance:0.38 dB/cm when straight and 0.56 dB/cm when *** properties make them ideal for smart sensors and flexible electronic ***-crystal analyses reveal that molecular stacking and intermolecular interactions are crucial to their elastic and piezochromic properties,providing insights for the design of future responsive materials.

摘要：利用VB语言和ANSYS二次开发语言APDL,开发了起重机金属结构疲劳寿命评估系统。其中,VB平台用来设计方便友好的人机交互界面,采用APDL语言开发起重机金属结构强度参数化分析模块。在结构疲劳寿命评估工具中,提供了基于Paris公式、Paris公式与Miner线性累积损伤理论相结合、热点应力法的三种不同寿命估算模型。最后以某桥式起重机桥架结构为对象,对所开发的软件工具功能进行了具体验证,结果表明该工具可以为起重机金属结构的数字化设计提供帮助。

摘要：With the continued expansion of oil and gas exploration,both in the eastern and western regions,the quality of seismic acquisition has become a key factor in oil and gas exploration in complex ***,conventional seismic acquisition methods cannot effi ciently avoid challenging acquisition locations and produce high-quality seismic *** this regard,based on the curvelet transform,this paper proposes an irregular seismic acquisition method,which utilizes the high-precision characteristics of the curvelet transform and simulated annealing algorithm to establish a method for the evaluation of the coherence of irregular sampling matrices and design of observation *** method was verifi ed using forward simulation and actual acquisition *** results suggest the superior quality of seismic data gathered in complicated areas through this method over those acquired using traditional methods,which can provide technical guidance for the design of observation systems in complex areas.

摘要：Conjugated polymers exhibit exceptional adaptability in flexible thin-film transistors,owing to their inherent flexibility and high charge ***,achieving conjugated polymers that demonstrate both high mobility and stability under mechanical stress,such as bending or stretching,remains a significant *** this study,we synthesized an ethylene-bridged conjugated polymer,PDPPVIDT,derived from methyl-side diketopyrrolopyrrole(DPP)and indacenodithiophene(IDT)aldehydes,using an environmentally friendly Knoevenagel condensation reaction under mild conditions,resulting in a moderate yield with promising *** characterization techniques revealed enhanced backbone planarity with stableπ-πstacking,while thin films of PDPPVIDT displayed a nearamorphous nature due to the conformational isomerism of the ethylene *** structure enables simultaneous charge transport and ***,flexible organic thinfilm transistor(OTFT)devices based on PDPPVIDT demonstrated excellent hole mobility(μh)reaching up to 1.70 cm^(2) V^(−1) s^(−1),along with high mechanical bending *** work underscores the potential of vinyl-bridged donor-acceptor conjugated polymers,highlighting the industrial adaptability of their production process and offering new insights for the design and synthesis of future flexible electronic materials.

摘要：反恐处突人员在处理城市暴力恐袭事件时需要同时应对刀刺及枪击的威胁,单一防护功能的防护服难以保障生命安全。根据防弹和防刺机理,将防弹和防刺材料进行组合设计可达到多功能的防护效果。目前基于组合材料的双防服研制方法主要为实验试错,设计效率低下,成本较高。针对这一问题,提出一种基于能量吸收准则的纤维混杂防弹防刺结构理论设计与优化方法,开展单一防护材料即浸胶芳纶布和超高分子量聚乙烯(Ultra high molecular weight Polyethylene,PE)无纬布的弹道实验和动态穿刺实验,计算单一材料的弹道动能吸收和穿刺动能吸收。基于能量守恒定律,设计给定8500 g/m^(2)面密度下的防弹防刺组合结构,得到防弹与防刺材料的层数范围。经过弹道及动态穿刺实验验证,理论计算安全余量充足,11层浸胶芳纶布加18层PE无纬布组合满足公安部《GA68—2019警用防刺服》及警用三级防弹要求且最为轻便,面密度8390 g/m^(2)。对防弹防刺结构进行优化设计,得到面密度为8370 g/m^(2)的最轻量组合结构(9层浸胶芳纶布加21层PE无纬布),研究成果可为双防服的工程设计与创新应用提供参考。

摘要：分析荆楚文化的传统符号、价值观和审美特征,提出将这些文化元素融入虚拟形象设计的方法。结合现代社会的审美需求,凸显时代精神的荆楚文化虚拟形象设计,可以促进荆楚文化的传承与发展,为数字媒体艺术领域贡献新的设计理念。