摘要：Inspired by the constitution of things in the natural world,three-dimensional (3D)nanofiber scaffold/cells complex was constructed via the combination of electrospinning technology and origami *** nanofiber boxes prepared by origami provided a limited space for the layer-by-layer nanofiber films,and the human fetal osteoblasts (hFOBs)seeded on the both sides of the nanofiber films were expected to facilitate the bonding,of the adjacent nanofiber films through the secretion of extracellular ***,the hFOBs presented 3D distribution in the nanofiber scaffold,and they can stretch across the gaps between the adjacent nanofiber films,forming the cell layers and filling the whole 3D nanofiber ***,a 3D block composed of electrospun nanofiber scaffold and cells was obtained',which possesses potential applications in bone tissue ***,we also created 3D nanofiber structures that range from simple forms to intricate architectures via origami,indicating that the combination of electrospinning technology and origami techniques is a feasible method for the 3D construction of tissue engineering scaffolds.

摘要：一直以来,由创伤、肿瘤切除和先天畸形引起的临界性骨缺损的临床治疗都存在极大的挑战,包括自体骨移植在内的传统骨缺损治疗方法,由于供体不足和免疫排斥反应等问题,已经不足以满足临床需求。而组织工程的发展改变了骨修复的传统治疗模式,基于人工和天然生物材料的各种组织工程复合支架被用于修复受损骨。与此同时,也有越来越多的学者认识到骨膜在骨缺损修复中的重要作用,尤其是组织工程支架的整合性和血管化。除了自体或异体骨膜移植,研究者将组织工程的理念融入到骨膜修复中,从结构和功能上模拟天然骨膜。从组织工程骨膜的不同设计理念出发,简单回顾了4类组织工程骨膜的研究进展,包括结构仿生型、微观结构仿生型、血管化型和外力刺激型,这些将为骨组织工程构建和骨膜深入研究提供理论基础并开启新的思路和方法。