摘要：One of the most important issues regarding the design of engineered landfill is landfill leachate that is produced when water passes through the waste. Landfill Leachate comprises a mass of different organic and inorganic components which can be dissolved or suspended. Regardless of the nature of the components, it poses a potential pollution on local land and surface waters. The present work was undertaken to assess, isolate, identify and characterize the hydrocarbon degrading bacteria associated with environmental samples collected from discharge of Ain Temouchent, Algeria. The samples (landfill leachate) were chemically and microbiologically analyzed using standard techniques. These organisms were further studied to determine the level of biodegrading activities on hydrocarbons as the single carbon source using enrichment medium under different physical and chemical parameters (pH, temperature, NaCl and tolerance of crude oil...). The identification of seven strains isolated named as Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas sp., Staphylococcus aureus, Staphylococcus xylosus, Staphylococcus hominis and Staphylococcus warnerii. These strains growth on a neutral medium and temperature between 30 °C and 37 °C, 5 at 6% of NaCl concentration, and can use a concentration of 5 at 10% of crude oil as sole source of carbon.