摘要：基于PCV2滚环复制原理,在分析PCV2不同基因亚型代表毒株基因组结构和序列的基础上,设计2对引物用于PCR扩增PCV2基因组序列。以从收集到的疑似PCV2感染的10份猪血清样品中提取的DNA为模板,用设计的引物扩增目的片段,并进行测序分析,1株为PCV2a型,4株为PCV2b型,5株为PCV2d型。每种基因型毒株中各选取一个样本,利用上述2对引物进行PCR扩增,并将扩增产物克隆至pEASY-Blunt simple载体中,通过双酶切连接2个PCR片段,构建含有约1500 bp重叠序列的PCV2基因组的质粒。通过脂质体转染法将含PCV2全基因组的质粒转染至PK-15细胞进行病毒拯救,经PCR和IFA两种方法验证,证明成功拯救出3个基因型的PCV2毒株。通过对PCV2全基因组结构及序列分析,该方法同样适用于PCV2g、PCV2h基因型病毒的感染性克隆构建。本研究建立了一种基于反向遗传学技术的PCV2感染性克隆的构建方法,为开展PCV2的病原学研究提供了技术支持。

摘要：为缓解我国黄土高原区干旱胁迫和控制水土流失,本研究采用裂区设计,生物炭施加模式(生物炭施加和无生物炭施加)为主区,耕作模式(打结垄、开敞垄和平作)为副区,研究不同生物炭施加模式和耕作模式对土壤水分、径流、泥沙流失、养分流失、紫花苜蓿(Medicago sativa L.)干草产量和水分利用效率的影响。试验结果表明,在2019和2020年紫花苜蓿生育期,与平作处理相比,开敞垄和打结垄处理的径流量、泥沙、全氮、全磷、有机质流失量分别减少20.1%~37.7%和60.1%~64.7%,54%~75.5%和77.1%~87.6%,44.4%~65.5%和49.0%~81.8%,36.7%~75.3%和48.6%~87.3%,48.2%~72.9%和58.6%~85.4%,土壤贮水量、紫花苜蓿干草产量和水分利用效率分别增加39.5~52.1和31.2~60.5 mm,26.2%~31.7%和26.5%~35.2%,10.07~14.86和12.14~16.55 kg·hm^(-2)·mm^(-1)。与无生物炭施加处理相比,生物炭施加处理的径流量、泥沙、全氮、全磷和有机质流失量分别减少48%~69%,94%~125%,12%~24%,28%~46%和35%~48%,土壤贮水量、紫花苜蓿干草产量和水分利用效率分别增加42.93~26.09 mm,106%~111%和1.96~6.90 kg·hm^(-2)·mm^(-1)。本研究表明,生物炭施加打结垄沟集雨种植是该区域适宜耕作模式。

摘要：虚假广告行为是现实生活中司空见惯的现象,但自从1997年被纳入刑法规制以来,被定为虚假广告罪而受刑罚处罚的尚不多见。而虚假广告罪网络虚假广告在理论和实践中还有很多问题值得深入研究,试对虚假广告的界定、本罪的主体和客体以及网络虚假广告等问题略抒浅见。

摘要：目的检验中文版医生用惊恐障碍严重度量表（PDSS）和患者用惊恐障碍严重度量表（PDSS—SR）及中文版惊恐相关症状量表（PASS）的信度和效度，提供中国惊恐发作患者的PDSS和PASS评分参照值。方法为多中心横断面设计；分别于基线、3—5d、4周时和6周时，对126例符合《美国精神障碍诊断与统计手册（第4版）》惊恐障碍诊断标准的中度以上成年患者进行PDSS、PDSS—SR和PASS测评，同时测定汉密尔顿焦虑量表（HAMA）、17项汉密尔顿抑郁量表（HAMD17）、临床总体印象严重度指数（CGI—S）、焦虑状态特质量表（STAI）和焦虑自评量表（SAS）。结果PDSS基线平均（16．48±4．02）分，Cronbach’s α系数0．74，重测一致性0．70～0．89，与HAMA和CGI-S有显著意义的相关（相关系数分别为0．35，0．46；P均〈0．01）；含2个因子。PDSS—SR基线平均（15．63±4．45）分，与PDSS他评总分的相关系数为0．78。PASS基线平均（14．17±4．19）分，量表内部一致性Cronbach’s α系数0．72，重测一致性0．64—0．77，与PDSS和PDSS—SR的相关系数分别为0．68和0．60。结论PDSS、PDSS—SR和PASS有较好的信度和效度，适合用于惊恐障碍临床严重程度的监测。

摘要：研究了基于运行参数在线监测的主管道断裂评定方法,建立了由热电势测试材料冲击功、裂纹撕裂断裂韧度预测和在线含缺陷管道断裂评定的成套技术方案,开发了专用的主管道断裂评定软件,并验证了某工况下使用该软件的计算结果与原设计要求偏差小于3%。

摘要：吸附/吸收式空气取水技术操作简易、取水能耗低,是缓解淡水资源短缺的有效措施。本文对吸附/吸收式空气取水技术近年来的研究进展进行了系统综述,介绍了吸附/吸收式空气取水技术的基本原理和主要材料类别,总结了吸附/吸收设备的材料放置结构、设备运行方式和应用场景。吸附/吸收材料包括传统的多孔结构材料、吸湿盐和新型有机空气取水材料。目前,各类研究已在提高材料的吸附性能、拓宽材料应用范围以及减少吸水-释水所需能耗方面取得重大进展。然而,吸附/吸收材料仍存在机械性能较差和无法型材化等问题,亟待后续更加深入的研究。吸附/吸收设备中的材料可通过平铺放置、中心轴放置、褶皱放置等多种结构形式进行空气取水应用,设备可采用一天一循环或一天多循环的模式运行。除了直接收集液态水以外,吸附/吸收式空气取水设备还可用于培育生态农场,为缓解地区淡水资源匮乏和食物短缺等问题提供了思路。

摘要：Based on the principles of the bioisosterism, combination of the active substructures of selective estrogen receptor modulators which are currently therapeutic agents available for the prevention and treatment of various estrogen dependent diseases, and structural optimization, a novel series of 2-aroyl-3-aryl-6,7-dihydro-5H-furo[3,2-g]- chromen derivatives was designed as potent selective estrogen receptor modulators via molecular docking. The target compounds have been synthesized, and characterized by 1R, proton NMR, ESI-MS, elemental analysis and evaluated for their antitumor activity against human osteosarcoma U2OS-EGFP-4FI2G cell line. Some target compounds showed good inhibition effects on U2OS-EGFP-4F12G cell line and the preliminary structure-activity relationships were discussed.

摘要：基于消费者认知视角和权变管理思想,利用我国上市公司数据实证分析慈善捐赠对广告营销与企业绩效之间关系的调节效应以及企业善待员工状况在这一过程中所发挥的深层调节影响。研究发现,慈善捐赠向消费者发出声誉信号,有助于提升广告营销投入的市场效果,慈善捐赠水平越高,广告营销促进企业销售增长的作用越强;然而企业善待员工水平较低时容易引起消费者对企业慈善动机真诚性的质疑,不利于捐赠行为增加积极的声誉资本,从而将削弱慈善捐赠对广告营销与销售增长之间关系的正向调节效应。

摘要：Unlike the previous research works analyzing the stability of the T-S （Takagi-Sugeno） fuzzy model, an extension on the stability condition of T-S fuzzy systems with a different strategy is provided. In the strategy a new variable, which is relative to the grade of fuzzy membership function, is introduced to the stability analysis and a new stability conclusion is deduced. The definition of stability condition in this paper is different from previous works, though they are similar in form. With the proposed method, the simulation in flight control law shows a better effectiveness.

摘要：An approach to design and implement the control function of LD (Ladder Diagram) in the hydropower simulation system based on all paths searching algorithm is proposed in this paper. LD is widely used as a programming language for PLC (Programmable Logic Controller), but it doesn’t be executed automatically in the hydropower simulation system which is a software system, and there is no compiler or interpretation for LD in it. The approach in this paper is not only to present a graphical interface to design LD, but also implement its control function through transforming it to a corresponding undigraph, in which, all paths be-tween two vertexes (live wire and null line) are searched by the proposed algorithm. An application example is presented to verify the validity of the algorithm and shows that the algorithm is correct and practicable. In addition, how to implement the control function based on object-oriented thought is introduced. The running time is shown at last, which proves that the system with the algorithm can meet the real-time request in the hydropower simulation system.