摘要：Cardiovascular diseases are the leading cause of morbidity and mortality throughout the world underlining the importance of efficient treatments including disease modeling and drug discovery by cardiac tissue ***,the predictive power of these applications is currently limited by the immature state of the ***,we developed gelatin hydrogels chemically crosslinked by genipin,a biocompatible crosslinker,as cell culture *** rat cardiomyocytes appear synchronous beatingwithin 2 days after seeding on ***,we applied the electrical stimulation as a conditioning treatment to promote the maturation of cardiomyocytes cultured on the *** results show that electrical stimulation improves the organization of sarcomeres,establishment of gap junctions,calcium-handling capacity and propagation of pacing signals,thereby,increase the beating velocity of cardiomyocytes and responsiveness to external *** above system can be applied in promoting physiological function maturation of engineered cardiac tissues,exhibiting promising applications in cardiac tissue engineering and drug screening.