摘要：Over the past few years, three photorespiratory bypasses have been introduced into plants, two of which led to observable in creases in photos yn thesis and biomass yield. However, most of the experiments were carried out using Arabidopsis under controlled environmental conditions, and the increases were only observed under low-light and short-day conditions. In this study, we designed a new photorespiratory bypass (called GOC bypass), characterized by no reducing equivalents being produced during a complete oxidation of glycolate into CO2 catalyzed by three rice-self-originating enzymes, i.e., glycolate oxidase, oxalate oxidase, and catalase. We successfully established this bypass in rice chloroplasts using a multi-gene assembly and transformation system. Transgenic rice plants carrying GOC bypass (GOC plants) showed significant increases in photosynthesis efficiency, biomass yield, and nitrogen content, as well as several other CO2-enriched phe no types under both greenhouse and field conditions .Grain yield of GOC plants varied depending on seeding season and was increased significantly in the spring. We further demonstrated that GOC plants had significant advantages under high-light conditions and that the improvements in GOC plants resulted primarily from a photosynthetic CO2-concentrating effect rather than from improved energy balance. Taken together, our results reveal that engineering a newly designed chloroplastic photorespiratory bypass could increase photosynthetic efficiency and yield of rice plants grown in field conditions, particularly under high light.

摘要：针对轨道交通弯道精细化和人性化设计问题,量化行人自适应特性指标及计算原理,采用行人可控实验采集相关数据,并运用基本图原理验证行人可控实验数据;开展弯道行人自适应特性研究,分析了弯道设计对行人自适应特性的影响.结果表明:弯道角度越大,行人自适应行为的稳定性越强,安全性越高;弯道半径的临界点为7 m,7 m前行人自适应特性随半径增大而趋于一致,稳定性增强,7 m后则降低;角度在[120°,160°]和半径[4,10]m范围下的行人自适应特性表现最稳定,效率最高;角度[100°,110°]与170°和半径[11,15]m次之;角度90°和半径1 m的行人稳定性最差,最不安全.在客流较大时,建议参考的弯道角度范围为[120°,160°]和半径范围为[4,10]m.

摘要：Inorganic-organic composite electrolyte is proved an effective way to enhance the overall performance of the ***,simply combining powder fillers with polymers is not sufficient for the application of composite *** this work,we designed an ultrathin organic-inorganic composite solid electrolyte with high mechanical strength and ionic conductivity,in which the inorganic li1.5Al0.5Ge1.5(PO_(4))3(LAGP)solid electrolyte is prefiberized into a three-dimensional nanofiber network to serve as a self-supporting skeleton for the polyethylene oxide(PEO)*** continuous skeleton structure not only significantly improves the mechanical strength of the PEO-based electrolyte,but also forms a continuous lithium-ion conduction path,promoting the rapid migration of lithium *** fiber-reinforced composite electrolyte has an ionic conductivity of 8.27×10^(−4) S cm^(-1) at 60°C and a tensile strength of up to 4.29 ***,it exhibits a reduced overpotential and stable long-term cycling performance over 1700 h when used in li/li symmetric *** liFePO_(4)(LFP)|li cell assembled with the fiber-reinforced composite electrolyte also delivers a specific capacity of about 142 mAh g^(−1) over 300 cycles at 0.5 C and maintains good cycling *** work provides a novel idea for designing the next generation of safe and reliable organic-inorganic composite solid-state electrolyte membranes.

摘要：目的宫颈癌FIGO分期存在一定的缺陷,血浆D-二聚体(D-D)水平与肿瘤预后密切相关。本研究探讨术前血浆D-D水平对早期宫颈癌术后复发的影响。方法试验设计属于前瞻性随访研究,以2009-01-01-2012-12-31广州市花都区妇幼保健院诊治的165例早期宫颈癌患者为研究对象。根据术前血浆D-D水平分为D-D升高组和D-D正常组,以电话随访为主,随访终点事件为宫颈癌复发。生存率估算应用Kaplan-Meier法,组间生存率曲线差异采用Log-rank检验,应用多变量Cox比例风险回归分析宫颈癌复发的独立危险因素。结果 D-D升高组67例(40.6%)。D-D正常组和D-D升高组中肿瘤直径≥4cm的比例分别为15.3%和29.9%,χ~2=5.037,P=0.025;淋巴结转移分别为35.7%和55.2%,χ~2=6.158,P=0.013;临床分期Ⅰ/ⅡA分别为47/51和20/47,χ~2=5.411,P=0.020。D-D正常组有11例复发,复发时间中位数为36(15~62)个月,5年无复发生存率为88.8%;D-D升高组有16例复发,复发时间中位数为25.5(6~50)个月,5年无复发生存率为74.0%。Kaplan-Meier生存曲线表明,D-D升高组无复发生存率显著低于D-D正常组,χ~2=4.805,P=0.028。单因素分析显示,组织学类型(HR=1.124,P=0.034)、淋巴结转移(HR=1.589,P=0.016)、FIGO分期(HR=1.854,P=0.007)、D-D水平(HR=1.395,P=0.023)和治疗方法(HR=0.891,P=0.043)为复发的危险因素。多变量Cox比例风险回归分析显示,淋巴结转移(HR=2.116,P=0.012)、FIGO分期(HR=4.421,P=0.001)、D-D水平(HR=2.125,P=0.039)和治疗方法(HR=0.912,P=0.045)是影响宫颈癌复发的独立危险因素。结论术前D-D水平是影响宫颈癌复发的危险因素,有助于更好地指导治疗、判断预后。

摘要：针对一种特殊用途重载轴承在使用时存在边缘效应、局部应力较大和使用寿命较低的问题,建立轴承的详细接触分析有限元模型并进行验证。采用工程对数凸型对滚子型线参数进行优选设计,用Python程序实现滚子节点网格参数自动更新,实现多方案的自动分析。对不同型面修型参数和滚子圆角半径进行多方案计算,得到了优选方案,避免边缘效应,并使轴承滚子和内圈应力下降了15%以上,效果显著。

摘要：为改善空心钢筋混凝土柱(HRC)的使用与受力性能,提出一种双壁波纹钢管混凝土(CFDCST)构件。本文共进行了4个大尺寸双壁波纹钢管混凝土短柱与2个空心钢筋混凝土短柱的轴压试验,关键试验参数为试件类型与波纹钢管类型。分析构件的破坏模式,对比不同参数下构件的承载力、峰值应变、延性系数等关键力学指标,获得荷载-波纹钢管应变关系曲线以揭示试件的约束机理并验证外波纹钢管类型的影响。试验结果表明:外波纹钢管可为夹层混凝土提供约束作用,且螺旋角越小,外波纹钢管的约束作用越强;内波纹钢管则主要为夹层混凝土内壁提供有效支撑,以保持截面的完整性。名义约束效应系数与空心率相近时,由于咬口滑移影响,大螺旋角双壁波纹钢管混凝土比小螺旋角双壁波纹钢管混凝土的峰值应力、峰值应变与延性系数分别降低15.5%、21.8%与16.7%。在总用钢量相近的情况下,小螺旋角双壁波纹钢管混凝土柱的承载力、峰值应变与延性系数分别比空心钢筋混凝土柱提高10.6%、36.2%与50.0%。基于试验结果,检验了3种典型承载力公式的适用性,并提出了相应设计建议。

摘要：将服务区间距信息、服务水平、高速公路所处地形条件等因素对服务区用地量的影响引入车辆连续行驶时间分布函数中,提出了基于连续行驶时间的服务区驶入率计算方法。考虑主线交通量的交通组成、不同车型的服务需求特性,计算服务区的服务交通量和服务客流量,并对停车场、公共厕所、加减速车道、餐厅等设施的用地面积分别进行计算,从而确定服务区的用地量计算模型。最后借用大广高速衡大段工可阶段OD调查数据,完成了对其沿线各服务区驶入率和各服务设施的合理用地量的计算,得到了各服务区的合理用地量。

摘要：目的克隆粉尘螨变应原Der f 2编码基因,并构建毕氏酵母表达体系,对表达产物进行圆二色分析。方法提取粉尘螨总RNA,根据GenBank中的序列设计并合成简并引物,PT-PCR扩增Der f 2全长基因,与pGAPZα-A载体连接后转化至*** Top10F',取阳性克隆并测序;将pGAPZα-A-Der f 2用BlnⅠ进行线性化,电转化至GS115感受态细胞,用IPTG进行诱导表达,采用SDS-PAGE验证表达产物,用His Trap HP亲和柱纯化重组蛋白Der f 2,进行圆二色谱扫描分析。结果 PCR扩增获得Der f 2编码基因,成功构建表达质粒pGAPZα-A-Der f 2,SDS-PAGE验证表明该质粒在GS115感受态细胞中正常表达,表达产物分子质量单位为14.9ku,重组蛋白Der f 2纯化后进行圆二色谱数据分析,其二级结构α-螺旋占41.2%,转角占10.3%,β-折叠占26.8%,不规则卷曲占21.7%。结论尘螨变应原Der f2在毕氏酵母中成功表达,为该变应原的进一步研究及规模生产和应用奠定了基础。

摘要：水情教育是国情教育的重要组成部分,水情教育高质量发展是生态环境可持续发展的重要保障,而高校是实施水情教育的重要阵地。通过对江苏涉水高校的问卷调查与访谈发现,江苏涉水高校在教育组织架构、教育内容设计、教学方法选择、教育评价与激励应用、育人成效等方面取得了显著进展,初步实现了水情教育的体系化建设。然而,实践中仍存在教育组织机制不完善、教育内容与方法适配度低、教育评价重知识传授轻技能实训、教育激励与学生需求不相容、学生水情素养发展不平衡等问题。从江苏水情教育高质量发展视域出发,提出了涉水高校水情教育实践创新路径,包括建立跨部门协同机制和信息共享平台、创新教育内容与方法、完善评价体系、优化激励机制、利用数字化工具促进现代化教学等,以提升水情教育的科学性、针对性与实效性,为培养具备全面水情素养的新时代人才提供有力支撑。

摘要：文中设计一款基于UCC28180芯片的低功耗高功率因数的有源功率因数校正模块来提高装置的功率因数,该模块最大输出功率为500 W。与现有APFC方案相比较,APFC控制器的功耗减少50%左右,功率因数校正超过0.95,可广泛应用于工业空调、照明、家用及办公电器等设备和场合。经过spice软件仿真,证明了设计的正确性和可靠性。