摘要：To develop fusion protein of a GnRH Fc fragment and the integrin targeting AP25 antitumor peptide for GnRH receptor-expressing cancer *** LMRAP fusion protein was constructed.A transwell invasion assay was *** gene mRNA and protein levels of GnRHR-I,α5β1,andαvβ3 in different cancer cell lines were *** proliferation was measured using a cell counting *** antagonist assay was performed on GnRH ***-tumor activity was evaluated with a mouse xenograft tumor ***(IHC)was applied to detect CD31 and CD34 *** characteristics were determined with an indirect competition *** developed bifunctional fusion protein LMRAP not only inhibited HUVEC invasion,but also inhibited proliferation of GnRHR-I,α5β1,and high expression cancer *** IC50 for LMRAP in the GnRH receptor was 6.235 x 10-4 mol/*** significantly inhibited human prostate cancer cell line22 RV1 proliferation in vivo and in *** significantly inhibited CD31 and CD34 *** elimination half-life of the fusion protein LMRAP was 33 h in *** fusion protein made of a GnRH Fc fragment and the integrin targeting AP25 peptide retained the bifunctional biological activity of GnRHR blocking,angiogenesis inhibition,prolonged half-life and good tolerance.

摘要：Dermal substitutes have provided a template for the regeneration and reconstruction of the ***,the healed skin tissue often exhibits abnormal morphology and functionality,including scarring and *** this study,a composite bioink composed of methacrylated gelatin(GelMA)and chitosan oligosaccharide(COS)was proposed for printing a dermal scaffold using digital light processing(DLP)*** GelMA/COS bioink exhibited suitable porosity,swelling,degradation rate,and mechanical *** inclusion of COS demonstrated antibacterial effects against both Gram positive and Gram-negative bacteria,while simultaneously fostering the proliferation of human dermal fibroblasts(HDFs).Additionally,the application of COS could effectively reduce the expression levels of fibrosis-related genes,such as collagen I,collagen III,and fibronectin *** three-dimensionally printed cell-laden dermal scaffold exhibited excellent shape fidelity and high cellular viability,facilitating the extension of HDFs along the scaffold and the simultaneous secretion of extracellular matrix ***,the HDF-laden dermal scaffold transplanted into full-thickness skin defect sites in nude mice was shown to accelerate wound closure,reduce inflammation,and improve wound ***,the DLP-printed dermal scaffold provides an appealing approach for effectively treating full-thickness skin defects in clinical settings.

摘要：Traditional tumor models do not tend to accurately simulate tumor growth in vitro or enable personalized treatment and are particularly unable to discover more beneficial targeted *** address this,this study describes the use of threedimensional(3D)bioprinting technology to construct a 3D model with human hepatocarcinoma SMMC-7721 cells(3DP-7721)by combining gelatin methacrylate(GelMA)and poly(ethylene oxide)(PEO)as two immiscible aqueous phases to form a bioink and innovatively applying fluorescent carbon quantum dots for long-term tracking of *** GelMA(10%,mass fraction)and PEO(1.6%,mass fraction)hydrogel with 3:1 volume ratio offered distinct pore-forming characteristics,satisfactorymechanical properties,and biocompatibility for the creation of the 3DP-7721 *** analysis and quantitative real-time fluorescence polymerase chain reaction(PCR)were used to evaluate the biological properties of the *** with the two-dimensional culture cell model(2D-7721)and the 3D mixed culture cell model(3DM-7721),3DP-7721 significantly improved the proliferation of cells and expression of tumor-related proteins and ***,we evaluated the differences between the three culture models and the effectiveness of antitumor drugs in the three models and discovered that the efficacy of antitumor drugs varied because of significant differences in resistance proteins and genes between the three *** addition,the comparison of tumor formation in the three models found that the cells cultured by the 3DP-7721 model had strong tumorigenicity in nude *** evaluation of the levels of biochemical indicators related to the formation of solid tumors showed that the 3DP-7721 model group exhibited pathological characteristics of malignant tumors,the generated solid tumors were similar to actual tumors,and the deterioration was *** research therefore acts as a foundation for the application of 3DP-7721 models in drug devel

摘要：Network design problems （NDPs） have long been regarded as one of the most challenging problems in the field of transportation planning due to the intrinsic non-convexity of their bi-level programming form. Furthermore, a mixture of continuous/discrete decision variables makes the mixed network design problem （MNDP） more complicated and difficult to solve. We adopt a surrogate-based optimization （SBO） framework to solve three featured categories of NDPs （continuous, discrete, and mixed-integer）. We prove that the method is asymptotically completely convergent when solving continuous NDPs, guaranteeing a global optimum with probability one through an indefinitely long run. To demonstrate the practical performance of the proposed framework, numerical examples are provided to compare SBO with some existing solving algorithms and other heuristics in the literature for NDP. The results show that SBO is one of the best algorithms in terms of both accuracy and efficiency, and it is efficient for solving large-scale problems with more than 20 decision variables. The SBO approach presented in this paper is a general algorithm of solving other optimization problems in the transportation field.

摘要：针对广告的两个基本特征:镜头切换较频繁和经常突显商标信息,提出一种基于镜头切换和文本检测的广告检测算法.该算法先对各相邻帧进行直方图差分,再对剪切、消融、淡入淡出和划变4种常见的镜头切换方式进行分析和识别,实现了镜头切换检测.对于镜头切换频率较低的广告,通过基于最大梯度差分的文本检测方法检测广告商标信息,实现了广告自动检测系统.实验结果表明,对于约15min的视频序列,该检测算法可获得83.32%的正确率,可初步满足广告检测的要求.

摘要：为解决制造企业可维修备件库存管理策略粗放、库存结构不合理的问题,构建了基于(S,Q)策略的可维修备件两级库存控制模型。结合制造企业实际情况,提出了基于(S,Q)策略的两级供应和维修保障模式,以节约成本为优化目标,以服务水平为约束,针对制造企业的重要备件,构建了基于Metric模型的可维修备件两级库存控制模型。在算法求解上,设计了多种群遗传算法求解订货批量和再订货点。利用企业数据进行实例分析,对所建立的库存控制模型与传统库存模型进行比较,验证了模型的有效性。结果分析表明,所构建的库存控制模型可以在保持服务水平的前提下,将库存成本降低48%,该模型可为大型制造企业的可维修备件库存管理提供决策支持。

摘要：塔式太阳能热电站存在着定日镜面形精度不足,反射光斑尺寸大的问题,限制着系统的转化效率和成本。针对该问题,通过光学分析建立理想定日镜面形模型,该面形为随时间与位置变化的抛物面的一部分;基于板壳力学提出一种平板弯曲机构,与传统镜面采取的一致刚度分布不同,对方形平板进行二维刚度分布设计,使简单、低成本的弯矩输入载荷可以实现定日镜所需面形;基于有限元分析、光线追迹、牛顿迭代优化等数值程序,计算机构对给定时刻太阳角的最优面形,以及在全年太阳角分布下静态最优化面形的平均光学效率;设计制造定日镜面形试验系统并进行了原理试验;仿真与试验结果表明,机构实现面形可有效减小反射光斑尺寸,效率高于普通平面镜,且结构简单成本低,易于制造与现场装调,为实际系统设计提供了参考思路。

摘要：随着游戏科技的日益发展和普及,网络游戏中的身体体验经历了前所未有的延伸与增强,身体议题亦在此间的交流与互动中占据着重要位置。“在场”于游戏之中被认为是尤为重要的品质,其在“虚拟实境”中体现为由技术媒介和文化符号所带来的沉浸式体验和文化感知。从唐·伊德的的身体理论与技术文化观出发,以角色扮演游戏《黑神话·悟空》为例,可以探索技术身体何以在游戏文化的熏陶下,经历“在场”递归,并最终演替为玩家在游戏世界中的文化身份象征。游戏作为虚拟文化工具,丰富了数字技术背景下传统文化的表现维度,使玩家关注到游戏背后的真实世界与文化图景,将虚拟赛博空间的文化意蕴延伸至现实空间。

摘要：传统的美术教学方式已不能满足艺术设计对基础教学的要求,我们要加强学生创造能力和设计意识的培养,完善美术课程在设计教学中的应用,培养符合社会需求的专业设计人才。

摘要：我们与将军一起考虑一套多代理人系统(质量) 线性时间不变(LTI ) 动力学和性能索引联合系统的行为的一个控制问题。在代理人之间的互联拓扑学与自我环作为一台未受指导的小型旋转式印刷机被建模，在每个系统是一个节点，在每个节点的控制行动是它的状态的功能和它的邻居的状态的地方。在这份报纸考虑的线性二次的管理者(LQR ) 控制问题能在反馈获得上由于块对角线限制被认为是一个结构优化问题。它被看那在最小化一个单个代理人系统的和性能的分布式的控制器平等者下面最小化多代理人系统的 LQR 性能限制。一个足够的条件以一套线性矩阵不平等(LMI ) 被介绍完成某些非最优的表演说明。除了使控制设计更适用， LQR 表演区域的观点被介绍并且分析，它被显示关于拉普拉斯算符矩阵的特征值凸。多代理人系统的 LQR 控制然后被变换成一套分系统的 LQR 控制，它作为系数与最小、最大的特征值合并二仅仅不平等限制。数字例子被举说明建议方法。