摘要：Precision genome editing is highly desired for crop *** recently emerged CRISPR/Cas technology offers great potential applications in precision plant genome engineering.A prime editing(PE)approach combining a reverse transcriptase(RT)with a Cas9 nickase and a“priming”extended guide RNA(gRNA)has shown a high frequency for precise genome modification in mammalian cells and several plant ***,the applications of the PE approach in dicot plants are still limited and *** designed and tested prime editors for precision editing of a synthetic sequence in a transient assay and for desirable alleles of 10 loci in tomato by stable *** data obtained by targeted deep sequencing also revealed only low PE efficiencies in both the tobacco and tomato *** assessment of the activities of the PE components uncovered that the fusion of RT to Cas9 and the structure of PE gRNAs(pegRNAs)negatively affected the cleaving activity of the Cas9 *** self-complementarity between the primer binding sequences(PBSs)and spacer sequence might pose risks to the activity of the Cas9 ***,modifying the pegRNA sequences by shortening or introducing mismatches to the PBSs to reduce their melting temperatures did not enhance the PE efficiency at the MADS-box protein(SlMBP21),alcobaca(SlALC),and acetolactate synthase 1(SlALS1)*** data show challenges of the PE approach in tomato,indicating that a further improvement of the PE system for successful applications is demanded,such as the use of improved expression systems for enriching active PE complexes.

摘要：LAVA联合ASPECT Studio赢得了越南胡志明市中央公园的国际竞赛,该项目计划于2020年开始建设。同时,LAVA将为该项目在未来三年的开发和建设提供服务。设计团队和合作方将共同创造一个真实存在的场所,搭建连接过去和未来的桥梁,建立起人类、自然与科技之间的联系。摒弃了传统拘谨的设计格局,LAVA的设计是随性的、多功能的、友好的、互相联系且贯通的。