摘要：The mammalian target of rapamycin(mTOR) is a critical component of the PI3K-AKT signaling pathway. It is highly activated in cervical cancer, which continues to pose an important clinical challenge with an urgent need for new and improved therapeutic approaches. Herein, we describe the structure-based drug discovery and biological evaluation of a series of m TOR kinase inhibitors as potential anti-cervical cancer agents. The results of enzymatic activity assays supported C3 as a potential m TOR inhibitor, which exhibited high inhibitory activity with an IC50 of 1.57 μM. Molecular docking and dynamics simulation were conducted to predict the binding patterns, suggesting relationships between structure and activity. The anti-proliferative assay against diverse cancer cell lines was displayed subsequently, revealing that C3 exhibited significant proliferation inhibition against cervical cancer cell He La(IC50=0.38μM) compared with other cell lines. Moreover, C3 could effectively reduce the expression of phospho-ribosomal S6 protein(p-S6) in He La cells in a dose-dependent manner. Noteworthily, m TOR signaling and other cellular pathways might contribute to the significant effect of C3 against cervical cancer simultaneously. These data indicated that C3 represented a good lead molecule for further development as a therapeutic agent for cervical cancer treatment.

摘要：为探索发酵对青稞淀粉品质的影响,采用酿酒酵母和嗜酸乳杆菌复合菌种进行青稞发酵,研究了发酵温度、时间和菌种接种量对直链淀粉含量、支链淀粉含量、糊化特性及溶解特性的影响,并通过正交设计优化发酵工艺参数。结果表明,复合菌种发酵青稞最佳工艺参数为:接种3%的酿酒酵母,在28℃下发酵2 h后,接种5%嗜酸乳杆菌,在37℃条件下继续发酵1. 5 d。与未发酵的青稞相比,发酵后青稞直链淀粉含量、膨胀力和溶解度均有所增加,起始糊化温度和峰值黏度明显下降,说明通过发酵可以改善青稞淀粉的品质,在一定程度上改善了青稞的加工特性。

摘要：在微机电系统(MEMS)制造中,深反应离子刻蚀(DRIE)过程的精度是影响器件特性的重要因素之一。本文设计了一种完全对称弹性梁结构的模态匹配式陀螺的原型器件,以此为对象研究了局域掩膜图形对于DRIE刻蚀过程的影响。器件的测试结果表明驱动和检测模态有明显的失配,该失配的发生原因除了气体阻尼,更主要来源于驱动和检测结构弹性梁尺寸的工艺偏差。在分析了实验过程及结果的基础上可以认为,除了典型的DRIE滞后效应等因素外,器件结构的局域掩膜效应加剧了工艺偏差:对称弹性梁结构周边的非对称掩膜图形导致了刻蚀气体分布的局部不均匀,增加了DRIE刻蚀的侧蚀偏差。