摘要：Since a typical mammalian cell contains only 200–300 picograms of proteins and the lack of a proteome amplification strategy[1],a practical and high-throughput approach for single-cell proteomics has long been awaited.A series of methods have been explored to make single-cell proteomics *** design of nanoPOTS and OAD chip-based processes minimizes sample preparation steps and volumes to reduce sample loss[2,3].