摘要：The main propos. of this.res.arch was.to develop a two-s.ep biodies.l production technique from animal fat as.a raw material. The developed proces. cons.s.s.ofes.erification and trans.s.erification s.eps. With s.ecial attention to optimize the firs. s.ep is.acid catalyzed es.erification to reduce free fatty acid content and the s.cond s.ep is.alkali catalyzed trans.s.erification for converting triglyceride to fatty acid methyl es.er or biodies.l. Animal fat containing 78.80 mg KOH/g of high acid value and molecular weight of 851 g/mol with the highes. oleic acid content was.us.d as.raw material. Res.ond s.rface methodology （Rs.） was.applied for the experiment des.gn. This.were 20 experiments.involving the three inves.igated variables.of methanol to animal fat ratio, amount of s.lfuric acid catalys. and reaction time that were s.udied on es.erification to optimize the condition for decreas.ng acid value in animal fat les. than 2 mg KOH/g. The animal fat with low acid value was.further experimented in trans.s.erification s.ep to obtain fatty acid methyl es.er or biodies.l. Animal fat biodies.l is.further inves.igated by determining its.fuel properties.according to the As.M s.andard tes. method.