摘要：A unique porcine extracel.ul.r matrix (ECM) derived injectabl. tissue construct with 100 nm or 20 nm gol. nanoparticl.s (AuNP) was devel.ped for muscul.skel.tal.tissue engineering appl.cations. ECM has been shown to encourage cel.ul.rity and tissue remodel.ng due to its rel.ase of growth factors whil. AuNP have been shown to reduce reactive oxygen species (ROS) l.vel.. Injectabl. tissue constructs were created by homogenizing decel.ul.rized porcine diaphragm tendon conjugated with 100 nm or 20 nm AuNP at 1x, 4x, and 8x concentrations. Extrusion force testing demonstrated that homogenized tissue constructs were injectabl. at an appropriate cannul. size and force. l.929 murine fibrobl.sts were used to measure cel. viabil.ty, cel. prol.feration, intracel.ul.r ROS l.vel., and cel. migration in response to constructs. Enhanced cel. viabil.ty and prol.feration are observed on 1 × 20 nm AuNP constructs. ROS assays demonstrate reduced cel.ul.r ROS concentrations from al. 20 nm AuNP constructs and from 8 × 100 nm AuNP constructs compared with constructs without nanoparticl.s. Cel.ul.r migration is higher towards 4 × 20 nm AuNP constructs compared with constructs without nanoparticl.s. Resul.s support the potential.use of a porcine ECM derived injectabl. tissue construct with AuNP as an injectabl. tissue construct to reduce infl.mmation and to promote tissue remodel.ng in muscul.skel.tal.tissue engineering appl.cations.