摘要：Probiotics are the treasure of the microbiology *** have been widely used in the food industry,clinical treatment,and other *** equivocal health-promoting effects and the unknown action mechanism were the largest obstacles for further probiotic’s developed *** recent years,various genome editing techniques have been developed and applied to explore the mechanisms and functional modifications of *** important genome editing tools,CRISPR-Cas systems that have opened new improvements in genome editing dedicated to *** high efficiency,flexibility,and specificity are the advantages of using CRISPR-Cas ***,we summarize the classification and distribution of CRISPR-Cas systems in probiotics,as well as the editing tools developed on the basis of ***,we discuss the genome editing of probiotics based on CRISPR-Cas systems and the applications of the engineered probiotics through CRISPR-Cas ***,we proposed a design route for CRISPR systems that related to the genetically engineered probiotics.