摘要：本文基于当前全球新型冠状病毒肺炎疫情形势,分析患者后期可能出现的功能障碍,初步设计在社区康复工作中进行评估以及干预的具体方案和注意事项,为日后帮助患者提升机体各项能力,提供一定的借鉴。

摘要：Conventional blood sampling for glucose detection is prone to cause pain and fails to continuously record glucose fluctuations in *** glucose monitoring based on implantable electrodes could induce pain and potential tissue inflammation,and the presence of reactive oxygen species(ROS)due to inflammationmay affect glucose *** technology is less invasive,yet microneedle adhesion with skin tissue is *** this work,we developed a microarrow sensor array(MASA),which provided enhanced skin surface adhesion and enabled simultaneous detection of glucose and H_(2)O_(2)(representative of ROS)in interstitial fluid in *** microarrows fabricated via laser micromachining were modified with functional coating and integrated into a patch of a three-dimensional(3D)microneedle *** to the arrow tip mechanically interlocking with the tissue,the microarrow array could better adhere to the skin surface after penetration into *** MASA was demonstrated to provide continuous in vivo monitoring of glucose and H_(2)O_(2) concentrations,with the detection of H_(2)O_(2) providing a valuable reference for assessing the inflammation ***,the MASA was integrated into a monitoring system using custom *** work provides a promising tool for the stable and reliable monitoring of blood glucose in diabetic patients.

摘要：Modern medicine is increasingly interested in advanced sensors to detect and analyze biochemical *** sensors based on potentiometric methods are a promising platform for monitoring physiological ions in biological *** semi-implantable devices are mainly based on single-parameter *** semi-implantable electrodes for multiparameter sensing have more restrictions on the electrode size due to biocompatibility considerations,but reducing the electrode surface area could potentially limit electrode *** study developed a semi-implantable device system comprising a multiplexed microfilament electrode cluster(MMEC)and a printed circuit board for real-time monitoring of intra-tissue K^(+),Ca^(2+),and Na^(+)*** electrode surface area was less important for the potentiometric sensing mechanism,suggesting the feasibility of using a tiny fiber-like electrode for potentiometric *** MMEC device exhibited a broad linear response(K^(+):2–32 mmol/L;Ca^(2+):0.5–4 mmol/L;Na^(+):10–160 mmol/L),high sensitivity(about 20–45 mV/decade),temporal stability(>2weeks),and good selectivity(>80%)for the above *** vitro detection and in vivo subcutaneous and brain experiment results showed that the MMEC system exhibits good multi-ion monitoring performance in several complex *** work provides a platform for the continuous real-time monitoring of ion fluctuations in different situations and has implications for developing smart sensors to monitor human health.

摘要：目的:建立单管检测肿瘤坏死因子-α(TNF-α)和内参GAPDH mRNA表达水平的双重荧光实时定量PCR方法。方法:脂多糖刺激小鼠巨噬细胞Ana-1后提取细胞RNA,经反转录合成c DNA,应用自行设计的不同荧光标记的TNF-α和GAPDH引物探针,经PCR扩增后,在FAM通道和CY5通道分别读取Ct值,同时应用基因测序技术对结果进行验证,并构建质粒标准品分析该方法的灵敏度和重复性。结果:双重荧光实时定量PCR法检测TNF-α的灵敏度达4×101拷贝/μL,检测线性范围可达6个数量级,批内和批间重复性好。结论:新方法消除了目的基因与内参的加样误差,节约成本,快速准确,适用于TNF-αmRNA的定量检测。

摘要：通过以新鲜辣椒为主要原料,加入花椒、生姜、香料、米酒、酵素等多种辅料,对低盐多营养状态下经低氧稳定二次发酵的新型发酵辣椒酱进行有害物质及其品质分析。结果表明,新型发酵辣椒酱在加工过程中不仅能产生大量对人体有益的物质,如氨基酸态氮、超氧化物歧化酶(SOD)、氨基酸(其中谷氨酸和天门冬氨酸含量最为显著),还能产生浓郁的酱香、酯香、醇香等特殊的风味,同时可以控制有害物质亚硝酸盐、黄曲霉毒素B1的产生及有害微生物(大肠菌群、酵母菌、霉菌、致病菌)的生长。

摘要：为提高航天飞行器铸件舱段在方案论证阶段的结构设计效率、设计质量和迭代效率,基于航天飞行器复杂曲面结构特点对铸件舱段结构特征参数进行研究,提出曲线比率法以描述纵筋参数化布局;定义骨架模型发布元素,以实现铸件舱段的Top-Down设计。提出航天飞行器铸件舱段结构快速参数化设计方法,通过定义特征命名规则和创建特征集合实现建模规范化,通过封装舱段设计知识实现设计快速化,通过创建特征参数和公式实现特征参数化。通过建立交互式铸件舱段结构快速设计环境,实现铸件舱段结构的快速实例化、快速修改,并以航天飞行器铸件舱段为例验证了所提方法的可行性和有效性。

摘要：介绍一种基于AVR单片机和USB通信协议的便携式多道核谱分析仪的设计。阐述了高性能A/D转换器、USB接口芯片与单片机的硬件接口设计,以及AVR单片机和上位机的软件设计。

摘要：针对固定门限方法在语音端点检测技术中的局限性,为了提高低信噪比下语音端点检测的鲁棒性和准确率,将自适应门限应用于分形维数的语音检测中,提出了一种新的语音端点检测算法.该算法通过对语音信号产生机制的分析,将分形维数用于语音起止点的检测中,设计了自适应门限,从而有效降低了噪声干扰对检测结果的影响,并实现了实时检测.仿真实验结果表明,在低信噪比的情况下,改进的端点检测算法比传统的短时能量检测算法可更准确有效地实现带噪语音的端点检测,而且对噪声干扰具有更好的鲁棒性.

摘要：目的 基于质量源于设计理念(quality by design,QbD)建立制川乌的超高效液相色谱(ultra high performance liquid chromatography,UHPLC)分析方法。方法 以制川乌中6个主要成分的定量分析为目标,选取柱温、醋酸铵溶液浓度、流速、pH值、初始乙腈浓度为考察因素进行Plackett-Burman试验设计,根据试验结果确定关键方法参数进行Box-Behnken实验,建立设计空间并选择置信度为95%的置信区间估计法考察设计空间的可靠性,最后选择准确性轮廓方法(accuracy profile,AP)进行方法学验证。以分离度为目标所建最佳模型为二次项式模型,以分析时间为目标所建最佳模型为线性模型。基于以上两个模型建立分析方法的设计空间,并采用置信区间估计对方法的可靠性进行评价。结果 结果显示,从设计空间内选择的30 mmol·L^(-1)的醋酸铵浓度(pH=10)和体积分数28%的初始乙腈比例,在10 min内可完成制川乌中6生物碱的完全分离,该分析方法的准确性、中间精密度、重复性、真实性都在可接受的范围内。结论 优化后的方法可以用于制川乌的UHPLC分析,QbD理念为开发并优化制川乌中6个生物碱的UHPLC分析方法提供了新的思路。

摘要：本文针对具有操纵面卡死、失效故障以及执行器饱和的飞翼飞行器纵向运动,考虑系统的预定动态性能,提出了一种自适应反步补偿跟踪控制方案.设计预定动态性能(prescribed performance bound,PPB)边界以保证系统的跟踪误差,采用二阶指令滤波器限制执行器的饱和,通过控制分配避免执行器故障后对横侧向运动的影响.所设计的自适应反步补偿跟踪控制律能够保证系统对参考信号的渐近跟踪.仿真结果表明了本文方法的有效性.