摘要：采用响应面分析法优化金银花凉茶的浸提工艺条件,以金银花、菊花、夏枯草和鱼腥草为原料,绿原酸得率为指标.在单因素试验基础上,应用Box-Benhnken中心组合设计试验,对浸提工艺影响较大的料液比、浸提时间、浸提温度3个工艺参数进行优化.结果表明:金银花等原料的绿原酸水提工艺的最佳条件为不粉碎原料、固定金银花1g,菊花、夏枯草和鱼腥草质量比为2∶2∶1共1.5g,料液比为1∶32,浸提时间为71min,浸提温度为78℃,浸提2次,在该条件下验证绿原酸得率达到3.01%,接近于预测值3.04%.研究表明,优化得到的回归模型具有良好的预测能力.

摘要：采用响应面分析法优化山楂中总黄酮超声辅助提取的工艺条件.以总黄酮(以金丝桃苷计)得率为指标,在单因素试验的基础上,应用了响应面中的Box-Benhnken模型设计试验,对提取工艺影响较大的山楂粉碎粒度、超声时间、料液比、超声功率4个工艺参数进行优化.结合实际操作,最佳的工艺条件为粉碎粒度40目,超声时间33min,料液比1∶33(g/mL),超声功率160W,在此条件下,总黄酮得率为5.21%,与预测值5.26%基本相符.

摘要：ABC transporters are molecular machines which power the solute transport using ATP *** structural biology of ABC transporters has been exploding for the last few years,and this study explores timelines and trends for various attributes such as structural tools,resolution,fold,sources,and group *** study also evidences the significance of mammalian expression systems,advancements in structural biology tools,and the developing interest of group leaders across the world in the remarkably progressing *** field started in 2002 and bloomed in 2016,and COVID years were really productive to the ***,the study explores 337 structures of 58 unique ABC transporters deposited in the PDB database from which P-glycoprotein has the largest number of ***,62%of total structures are determined at the resolution of 3-4Åand 53%of structures belong to fold IV *** progressive advancements in the field,the field is shifting from prokaryotic to eukaryotic sources and X-ray crystallography to cryoelectron *** the nutshell,this study uniquely provides the detailed snapshot of the field of structural biology of ABC transporters with real-time data.