摘要：In order to satisfy the requirements of large workspace and high dexterity for processing equipment of oversized cylindrical boxes′spherical crown surfaces in the aerospace industry,a novel serial-parallel hybrid processing robot mechanism is *** degrees of freedom of the 5PUS-(2UR)PU parallel mechanism are obtained by using the screw *** inverse kinematics of the hybrid mechanism are analyzed and the velocity Jacobian matrix is ***,the constraints of the main factors influencing workspace of the mechanism are given,and the position and posture workspace are ***,the dexterity and stiffness performance of the mechanism is analyzed based on the Jacobian *** virtual prototype is established,and the theoretical calculation and simulation analysis of the hybrid mechanism with arc curve as the processing trajectory are carried out by using Matlab and Adams *** research results show that the mechanism can satisfy the requirements of large workspace and high dexterity of oversized cylindrical boxes′spherical crown surface processing,and has feasibility and practical application value.

摘要：Directed peptides C-terminal modification enabled by the engineered biomolecular catalyst-peptide amidase 12 B has been achieved via computational protein engineering. The engineered enzyme exhibits great promising potential in the C-terminal modification of opioid peptides using prop-2-yn-1-amine(PYA) or prop-2-en-l-amine(PEA) as the nucleophile. A variety of opioid peptides could be readily functionalized at the C-terminal chain in high yield in a mild and selective manner. Notably, modified opioid peptides bearing alkynyl moiety could be further functionalized through well-established click reaction.

摘要：[目的/意义]基于用户体验五要素模型分析用户体验公共数据开放平台的影响因素,以期帮助平台完善建设,实现用户体验的升级。[方法/过程]基于用户体验五要素模型进行要素析出、假设设计与模型构建,利用问卷进行信息搜集,通过SEM模型与NCA方法对数据进行路径检验、假设验证及必要性分析。[结果/结论]在用户体验五要素当中,内容需求、交互设计、界面设计、视觉表现均对用户体验具有充分的显著性影响;在必要性上则略有不同,据瓶颈水平分析可知,必要性程度由高到低分别是内容需求、视觉表现、界面设计及用户需求。

摘要：合成微生物组是指运用合成生物学方法构建的功能菌群.合成微生物组以代谢通路模块化为核心特征,每个代谢模块的工作由一个菌株完成,从而实现多个菌株的分工与合作.与单菌株相比,合成微生物组具有降低菌株代谢负担与遗传改造难度、提供多样的元件表达平台、实现'即插即用'的模块替换等优势.在合成生物学与微生物组学快速发展、交汇融合的影响下,合成微生物组已成为近些年微生物领域新的研究热点,在生物合成平台化合物、复杂大分子以及生产生物燃料等方面具有广阔的应用前景.本文介绍了合成微生物组的设计原理与优势,总结了近些年的主要研究成果,阐述其目前面临的挑战与机遇,最后对其未来的发展进行展望.

摘要：针对使用线性CCD作为图像采集模块的类人机器人图像获取时间长、效率低等缺点,设计一种基于OpenMv的类人机器人。类人机器人投篮系统设计首先完成软硬件设计,然后将采集到的图像转为HSV颜色模型,利用图像分割技术将目标与背景进行分割,进而计算质心坐标,最后通过专家控制策略输出得到相应的信号调整机器人的动作。实验表明:该系统具有较高的可靠性、适应性及有效性。

摘要：针对综合能源系统分布式调度过程中的隐私泄露问题,考虑分布式调度节点功率受限特性,提出了一种基于同态加密的完全一致性低碳经济调度方法。首先,对系统中供能单元和用能负载进行功率分析,建立综合经济性和低碳性的能源系统调度模型,引入供需不匹配状态估计变量,提出一种完全分布式一致性低碳经济调度算法。其次,利用同态密码学设计基于同态加密理论的信息交互策略,使节点在不泄露自身隐私数据的前提下与邻居节点安全地交换状态信息。同时同态加密技术允许直接在密文上进行运算,并确保解密后的结果与明文的直接计算结果一致,从而保证了低碳经济调度的准确性。最后,通过数值仿真实验验证了所提调度算法的有效性。

摘要：可数据化是现代生命科学研究,尤其是合成生物学的一项关键特性。酶作为生命体内催化生化反应的关键分子,其数据化对推动生命科学的基础研究和实际应用都有重要意义。当下商品化的酶大都来源于微生物,建立微生物酶资源数据库不仅可以为酶的分类提供标准参考,还可以指导新型催化元件的挖掘、改造与从头设计。本综述对国际上已有的酶资源数据库建设与发展作了简要介绍,并对基于数据库的微生物酶资源利用作出展望。

摘要：根据其它植物atp6、cob和coxⅡ基因保守区设计特异引物,利用RT-PCR技术从‘国庆1号’温州蜜柑cDNA中分别扩增出特异性片段,将其克隆至pBluescript(sk+)载体上进行测序。同源性分析表明,所克隆的atp6、cob和coxⅡ与其他植物的对应氨基酸区域同源性分别达到85%、98%和96%以上。RT-PCR分析表明它们在叶片、花瓣以及不同时期的花蕾中都有表达,在幼果中没有表达。Southern分析表明它们在温州蜜柑基因组DNA中为多拷贝。

摘要：Organic Rankine Cycles(ORCs) are an effective way to produce electricity from low-grade heat sources, which cannot be effectively obtained using conventional high-temperature Rankine cycles. Due to the lack of available information regarding the real Organic Rankine Cycle units on industrial level, off-design simulation under diversified operating conditions plays a significant role for both the system performance prediction and control strategy design. This paper summarizes the theoretical basis, modeling approaches and tools for ORC off-design simulations. Firstly, a review was conducted on the individual state-of-the-art convective heat transfer correlations and void fraction models. Secondly, different kinds of modeling approaches and simulation tools were proposed, highlighting their relevant characteristics, and were categorized for their specific applications. Moreover, an in-depth analysis of technical challenges related to various applications and focusing on the model accuracy and complexity, computational efficiency, as well as the model compatibility were extensively described and discussed. Finally, the current research trends in this field and the development for further investigations were presented.

摘要：为研究大直径钢筋装配式混凝土框架抗震性能,对三层两跨大直径钢筋装配式混凝土足尺框架进行拟动力试验,分析框架在地震设防三标准下的破坏形态、动力时程反应及抗震性能指标。研究结果表明:多遇地震作用下,框架并未出现裂缝,处于弹性状态;设防地震作用下,框架出现微裂缝,试验结束时裂缝闭合;罕遇地震作用后,框架进入弹塑性状态。以承载力和残余变形确定使用性能时,多遇地震作用下达到抗震性能3(完好);设防地震作用下,达到抗震性能4(轻到中等破坏);罕遇地震作用下,达到抗震性能4(不严重破坏);各地震工况下,框架底部剪力-层间位移角曲线基本呈线性,具有较好的滞回性能;罕遇地震后,柱主筋均未屈服,一、二层梁端主筋屈服,实现了“强柱弱梁”的设计目标。同时,对大直径钢筋框架和普通直径钢筋框架进行有限元对比分析,两者各层层间位移以及层间剪力均值相对误差分别为-5.73%和-1.41%,动力性能接近。