摘要：The global increase in the prevalence of drug-resistant bacteria has necessitated the development of alternative treatments that do not rely on conventional antimicrobial *** bacteriophage-derived lytic enzymes in antibacterial therapy shows promise;however,a thorough comparison and evaluation of their bactericidal efficacy are *** study aimed to compare and investigate the bactericidal activity and spectrum of such lytic enzymes,with the goal of harnessing them for antibacterial ***,we examined the bactericidal activity of spanins,endolysins,and holins derived from 2 Escherichia coli model phages,T1 and *** these,T1-spanin exhibited the highest bactericidal activity against ***,we expressed T1-spanin within bacterial cells and assessed its bactericidal activity.T1-spanin showed potent bactericidal activity against all clinical isolates tested,including bacterial strains of 111 ***,2 Acinetobacter spp.,3 Klebsiella spp.,and 3 Pseudomonas *** contrast,T1 phage-derived endolysin showed bactericidal activity against *** and ***,yet its efficacy against other bacteria was inferior to that of ***,we developed a phage-based technology to introduce the T1-spanin gene into target *** synthesized non-proliferative phage exhibited strong antibacterial activity against the targeted *** potent bactericidal activity exhibited by spanins,combined with the novel phage synthetic technology,holds promise for the development of innovative antimicrobial agents.