摘要：Nucleocapsid(N) protein plays crucial roles in the life cycle of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2), including the formation of ribonucleoprotein(RNP) complex with the viral *** we reported the crystal structures of the N-terminal domain(NTD) and C-terminal domain(CTD) of the N protein and an NTD-RNA complex. Our structures reveal a unique tetramer organization of NTD and identify a distinct RNA binding mode in the NTD-RNA complex, which could contribute to the formation of the RNP complex. We also screened small molecule inhibitors of N-NTD and N-CTD and discovered that ceftriaxone sodium, an antibiotic, can block the binding of RNA to NTD and inhibit the formation of the RNP complex. These results together could facilitate the further research of antiviral drug design targeting N protein.

摘要：利用传统定位系统对高压电缆局部绝缘老化故障进行定位,存在定位误差大,定位效率低的问题。针对上述问题,设计一个新型的高压电缆局部绝缘老化故障智能定位系统。由中心处理器、DTE数据通信设备和系统前端信号/数据采集设备构建系统整体框架,根据该框架设计系统软硬件,系统硬件包括传感器、通信设备和中心处理器,系统软件包括故障信号采集处理、故障信号传输、故障定位三大模块。结果表明:与传统绝缘老化故障智定位系统相比,利用本系统对高压电缆局部绝缘老化故障进行智能定位,定位误差平均降低1.28 m,定位准确性提高,定位时间缩短10.2 s,定位效率提高,由此可知本系统整体功能性要好于传统系统。