摘要：The OsLpa1 gene（LOC_Os02g57400） was identified to be involved in phytic acid（PA） metabolism because its knockout and missense mutants reduce PA content in rice grain. However, little is known about the molecular characteristics of OsLpa1 in rice and of its homologues in other plants. In the present study, the spatial pattern of OsLpa1 expression was revealed using OsLpa1 promoter：：GUS transgenic plants（GUS： β-glucuronidase）; GUS histochemical assay showed that OsLpa1 was strongly expressed in stem, leaf, and root tissues, but in floral organ it is expressed mainly and strongly in filaments. In seeds, GUS staining was concentrated in the aleurone layers; a few blue spots were observed in the outer layers of embryo, but no staining was observed in the endosperm. Three OsLpa1 transcripts（OsLpa1.1, OsLpa1.2, OsLpa1.3） are produced due to alternative splicing; quantitative reversetranscriptase polymerase chain reaction（RT-PCR） analysis revealed that the abundance of OsLpa1.3 was negligible compared with OsLpa1.1 and OsLpa1.2 in all tissues. OsLpa1.2 is predominant in germinating seeds（about 5 times that of OsLpa1.1）, but its abundance decreases quickly with the development of seedlings and plants, whereas the abundance of OsLpa1.1 rises and falls, reaching its highest level in 45-d-old plants, with abundance greater than that of OsLpa1.2 in both leaves and roots. In seeds, the abundance of OsLpa1 continuously increases with seed growth, being 27.5 and 15 times greater in 28-DAF（day after flowering） seeds than in 7-DAF seeds for OsLpa1.1 and OsLpa1.2, respectively. Transient expression of chimeric genes with green fluorescence protein（GFP） in rice protoplasts demonstrated that all proteins encoded by the three OsLpa1 transcripts are localized to the chloroplast.

摘要：In order to design a complex laser resonator with multi-parameters, the method of particle swarm optimization(PSO) algorithm is employed. The parameters influencing the resonator stability and mode size distribution are taken into consideration, and the stability criteria index and the mode size distribution are used as target values. The absolute values of the differences between practical and the target values are set as the fitness function for the PSO. By minimizing the fitness function, a laser resonator with the optimized cavity parameters can be found. The analyses for the design example demonstrate the feasibility and validity of the PSO method in the computer aided design of multi-parameters laser resonator. Applying PSO algorithm in the intelligent design of solid state laser resonators can realize the transition from manual trial-and-error to computer intelligent design of the laser resonators.

摘要：基于修正平均应力法,分析了TC17钛合金压气机轮盘型面尺寸参数对径向和周向破裂转速的影响,并对破裂模式的转变进行预判。进而设计了针对径向破裂模式的轮盘试验件,并开展了TC17钛合金压气机轮盘的破裂试验。轮盘破裂试验结果与修正平均应力法计算结果吻合较好,计算的破裂转速储备与试验结果的误差由修正前的7.24%下降为修正后的0.72%,可为工程应用提供依据。

摘要：针对煤矿工程人员对钻孔瓦斯抽采工艺认识及在安全培训中的不足,以淮南矿业集团潘二矿底板瓦斯抽采场为例,基于三维可视化与虚拟仿真技术进行了底板穿层钻孔瓦斯抽采工艺仿真系统的设计与开发,利用3DMAX三维建模软件对矿区煤岩层赋存状况及钻机、注浆泵等设备进行三维模型的创建,提出了系统设计开发的工艺流程,开发了基于三维可视化及虚拟仿真技术的穿层钻孔瓦斯抽采仿真系统。

摘要：为探讨乙二醇作为蓄冷剂的蓄冷库用于果蔬贮藏保鲜的可行性,研究人员设计了包含制冷循环系统、蓄冷剂循环系统和保鲜库制冷系统三个循环的乙二醇蓄冷库。按照国家标准冷库热工性能试验方法对研制的蓄冷库的围护结构传热系数、冷风机制冷量、耗电量和库内温度波动等冷库性能进行了分析,结果表明,研制的乙二醇蓄冷库,围护结构传热系数为0.20 W/(m2·℃),冷风机制冷量5.4 kW,耗电量35.48 kW·h·d-1,库内温度不均匀性2.8℃,平均温度波动±1.0℃,达到了果蔬贮藏保鲜设施的技术要求。

摘要：针对机械产品设计强经验、弱理论的特点,提出了一种以实例推理为主,结合知识库辅助设计的快速设计方法,论述了基于实例推理技术的机械产品快速设计方法的内涵与体系结构,重点研究了基于实例推理的实例库构建,知识库构建,工程图调整技术。采用基于实例的快速设计方法,利用三维CAD平台构建了门式起重机快速设计系统,实现了门式起重机产品的快速设计。

摘要：在大规模定制思想指导下,研究了系统模块化设计,并采用参数化设计技术、装配草图技术、工程图调整技术,以VB为开发工具,SolidWorks2007为开发平台,开发了门式起重机模块化快速设计系统(MRDSGC),缩短了新产品的开发周期,提高了设计效率和质量,使起重机生产企业快速响应个性化的市场要求。

摘要：介绍了对1000 MW机组塔式锅炉水冷壁高温腐蚀情况进行的检查,通过试验论证对产生高温腐蚀的原因进行分析,并对水冷壁高温腐蚀采取了相应的治理措施,总结了解决水冷壁高温腐蚀的有效措施,期望通过文中的研究能够对提高锅炉的安全稳定运行有所帮助。

摘要：为探究边境地区土地利用多功能之间的交互关系,基于生态位宽度、耦合协调度和权衡强度模型分析广西边境地区2000—2018年土地利用“三生”功能的时空演变及协调/权衡关系特征,并分区提出优化建议。结果表明:(1)广西边境地区“三生”功能时空分异特征显著,生产功能生态位宽度以扩张状态为主,呈“东南高、西北低”的梯度变化格局;生活功能生态位呈萎缩后扩大趋势;生态功能生态位宽度呈现萎缩态势,呈“双高多平”空间布局。(2)土地利用功能耦合协调类型区分为濒临失调型及勉强协调区,总体由濒临失调型向勉强协调型演变,勉强协调区主要为经济相对繁荣区,濒临失调型集聚在西部生态环境脆弱山区;两两功能权衡强度较低为防城区,权衡强度较高为那坡县、龙州县。(3)全区可划分为低协调区、生产—生活功能优势区、生产—生态功能优势区、生态—生活功能优势区、生产功能优势区、生态功能优势区、生活功能优势区并提出针对性措施。广西边境地区土地利用“三生”功能之间相互作用关系整体往协调态势发展,后续发展应注重以生态优先为导向。

摘要：研究了涕灭威与灭多威的遗传毒性.在去离子水中分别加入涕灭威与灭多威标准品,涕灭威设计0.002、0.02、0.2、2、20μg/L共5个剂量组,灭多威设计0.02、0.2、2、20、200μg/L共5个剂量组.用微核试验检测其对鲤鱼血红细胞微核的诱发效应,采用Ames试验检测2种农药的致突变性,采用彗星试验检测其对人外周血淋巴细胞DNA的损伤效应,根据3种毒理学试验结果综合分析涕灭威与灭多威的遗传毒性.结果表明,所有剂量组这2种农药未诱导鲤鱼血红细胞微核率的明显上升(p>0.05);2种农药各剂量组回变菌落数均未超过自发回变组的2倍,但在非代谢活化条件下,涕灭威2～20μg/L与灭多威20～200μg/L剂量组的TA97菌株回变菌落数分别达到(129.17±17.00)、(129.50±18.28)、(109.83±10.80)和(114.17±9.37)个/皿,明显高于自发回变组(p<0.05,p<0.01).在代谢活化条件下,灭多威200μg/L组的TA100与TA102菌株的回变菌落数为(147.83±23.29)、(275.83±20.63)个/皿,均高于自发回变组(p<0.05);在彗星试验中发现,高浓度的涕灭威与灭多威均可导致人外周血淋巴细胞的DNA损伤,涕灭威20μg/L组,灭多威200μg/L组的尾部DNA(%)、尾长、Olive尾距3个指标经统计学分析,高于去离子水对照组(p<0.01).研究未见涕灭威与灭多威对鲤鱼血红细胞产生明显的染色体损伤效应,虽未见其明显的致突变性,但在高浓度下存在一定的致突变的风险,并且这2种农药可能导致人外周血淋巴细胞的DNA损伤,因此涕灭威与灭多威污染对水环境和人体健康可能存在一定的远期危害.