摘要：Geopolymer is produced through the polymerization of active aluminosilicate material with an alkaline activator,leading to the formation of a green,inorganic polymer *** concrete(GPC)has become a promising low-carbon alternative to traditional Portland cement-based concrete(OPC).GPC-bonded reinforcing bars offer a promising alternative for concrete structures,boasting excellent geopolymer binder/reinforcement bonding and superior corrosion and high-temperature resistance compared to Portland ***,due to differences in the production process of GPC,there are distinct engineering property variations,including bonding *** literature review provides an examination of the manufacturing procedures of GPC,encompassing source materials,mix design,curing regimes,and other factors directly influencing concrete ***,it delves into the bond mechanism,bond tests,and corresponding results that represent the bond *** main conclusions are that GPC generally has superior mechanical properties and bond performance compared to ordinary Portland cement concrete(OPC).However,proper standardization is needed for its production and performance tests to limit the contradictory results in the lab and on site.

摘要：目的建立柯萨奇病毒A组5型(CVA5)特异的一步法实时荧光反转录聚合酶链反应(RT-PCR)核酸检测方法。方法以流行于遵义地区的CVA5病毒株和NCBI基因库中随机下载的CVA5 VP1基因序列作为参考序列,分析这些序列的保守区域,在其保守区设计特异性的引物与Taqman探针,建立检测CVA5的一步法实时荧光RT-PCR。通过构建含CVA5 VP1保守区域的重组质粒,绘制标准曲线,并对检测方法的灵敏度、特异性、重复性及临床样本检出限进行评价。利用该方法对遵义地区273份未明确分型的肠道病毒阳性样本进行检测,将检出的部分CVA5阳性产物进行测序。结果成功建立一种基于实时荧光RT-PCR的CVA5检测方法,该方法在10^(4)~10^(9)copy/mL范围内具有良好的线性关系(R 2>0.999),平均扩增效率为102.26%。灵敏度高,对临床样本的检出限为103copy/mL。该方法的特异性强,与柯萨奇病毒A组2型(CVA2)、柯萨奇病毒A组4型(CVA4)、柯萨奇病毒A组6型(CVA6)、丙型肝炎病毒、呼吸道合胞病毒和鼻病毒等均不发生交叉反应。重复性实验的变异系数低于1.5%。273份未明确分型肠道病毒阳性样本中共检出25份CVA5,占未明确分型阳性样本的9.16%。结论该研究建立的一步法CVA5RT-PCR检测方法灵敏度高、特异性强、重复性好,可用于CVA5的检测。