摘要：AIM To investigate the enhanced cytotoxic T lymphocyte responses against pancreatic cancer (PC) in vitro induced by dendritic cells (DCs) engineered to secrete anti-DcR3 monoclonal antibody (mAb). METHODS DCs, T lymphocytes and primary PC cells were obtained from PC patients. DCs were transfected with a designed humanized anti-DcR3 monoclonal antibody heavy and light chain mRNA and/or total tumor RNA (DC-tumor-anti-DcR3 RNA or DC-total tumor RNA) by using electroporation technology. The identification, concentration and function of anti-DcR3 mAb secreted by DC-tumor-anti-DcR3 RNA were determined by western blotting and enzyme-linked immunosorbent assay. After co-culturing of autologous isolated PC cells with target DCs, the effects of secreting anti-DcR3 mAb on RNA-DCs' viability and apoptosis were assessed by MTT assay and flow cytometry. Analysis of enhanced antigen-specific immune response against PC induced by anti-DcR3 mAb secreting DCs was performed using a Cr-51 releasing test. T cell responses induced by RNAloaded DCs were analyzed by measuring cytokine levels, including IFN-gamma, IL-10, IL4, TNF-alpha and IL-12. RESULTS The anti-DcR3 mAb secreted by DCs reacted with recombinant human DcR3 protein and generated a band with 35 kda molecular weight. The secreting mAb was transient, peaking at 24 h and becoming undetectable after 72 h. After co-incubation with DCtumor- anti-DcR3 RNA for designated times, the DcR3 level in the supernatant of autologous PC cells was significantly down-regulated (P < 0.05). DCs secreting anti-DcR3 mAb could improve cell viability and slow down the apoptosis of RNA-loaded DCs, compared with DC-total tumor RNA (P < 0.01). The anti-DcR3 mAb secreted by DC-tumor-anti-DcR3 RNA could enhance the induction of cytotoxic T lymphocytes (CTLs) activity toward RNA-transfected DCs, primary tumor cells, and PC cell lines, compared with CTLs stimulated by DC-total tumor RNA or control group (P < 0.05). Meanwhile, the antigen-specific CTL respon

摘要：针对很多约束优化问题的最优解位于可行域的边界上或其附近的特点,提出了一种新的遗传算法.算法将种群中的可行解和不可行解分别存贮在两个容器中,新设计的交叉算子(内外交叉法)尽量让可行域内的可行解与可行域外的不可行解交叉,并顺着有利的方向一维搜索到可行域边界,此举既增大了个体接近全局最优解的几率,又增强了算法的收敛速度;粒子群变异法则吸取粒子群(PSO)算法的优点,让粒子沿粒子自身历史最优和全局最优的方向变异,而选择算子则采取了保留固定比例不可行解的方法.仿真结果证明了算法能够在种群规模小,迭代次数少的情况下迅速接近或找到全局最优解.

摘要：Objective: As one of the most popular designs used in genetic research, family-based design has been well recognized for its advantages, such as robustness against population stratification and admixture. With vast amounts of genetic data collected from family-based studies, there is a great interest in studying the role of genetic markers from the aspect of risk prediction. This study aims to develop a new statistical approach for family-based risk prediction analysis with an improved prediction accuracy compared with existing methods based on family history. Methods: In this study, we propose an ensemble-based likelihood ratio(ELR) approach, Fam-ELR, for family-based genomic risk prediction. Fam-ELR incorporates a clustered receiver operating characteristic(ROC) curve method to consider correlations among family samples, and uses a computationally efficient tree-assembling procedure for variable selection and model building. Results: Through simulations, Fam-ELR shows its robustness in various underlying disease models and pedigree structures, and attains better performance than two existing family-based risk prediction methods. In a real-data application to a family-based genome-wide dataset of conduct disorder, Fam-ELR demonstrates its ability to integrate potential risk predictors and interactions into the model for improved accuracy, especially on a genome-wide level. Conclusions: By comparing existing approaches, such as genetic risk-score approach, Fam-ELR has the capacity of incorporating genetic variants with small or moderate marginal effects and their interactions into an improved risk prediction model. Therefore, it is a robust and useful approach for high-dimensional family-based risk prediction, especially on complex disease with unknown or less known disease etiology.

摘要：为推动川式预制菜产业的发展,同时提高花椒芽利用率,以羊肉、花椒芽、鱼汤冻为原料,设计了一款川式风味的羊肉肉燕产品。在红薯淀粉添加量、小麦淀粉添加量、食盐添加量3个单因素试验的基础上,以感官评分为评价指标,通过正交试验确定了肉燕皮的最佳配方为羊肉100 g、蛋清20 g、糯米粉10 g、小苏打2 g、红薯淀粉45 g、小麦淀粉70 g、食盐3 g。为了优化馅料配方,在鱼汤冻添加量、花椒芽添加量、花椒粉添加量3个单因素试验的基础上,以感官评分为评价指标,设计响应面试验与验证试验,确定了肉燕馅料的最佳配方为羊肉30 g、食盐1 g、鱼汤冻23.15 g、花椒芽8.8 g、花椒粉0.7 g。在此条件下制得的川式羊肉肉燕水分含量为(35.33±1.26)%,脂肪含量为(19.68±0.76)g/100 g,粗蛋白质含量为(10.71±0.38)g/100 g,淀粉含量为(6.94±0.23)g/100 g,天冬氨酸、脯氨酸、酪氨酸的含量分别为(8.79±0.22),(7.33±0.21),(10.09±0.17)mg/g。

摘要：提出了一种新型的数字式测力系统的设计方案 ,该系统以单片机作为中心控制单元 ,利用了石英谐振器力-频特性 ,采用了多周期同步法测频、数字滤波等技术 ,得到了高精度、高稳定性和响应快速的力传感数据 ,设计简单 ,工作可靠。

摘要：在账本公开、多方共识情况下确保交易身份的隐私保护是区块链技术面临的主要挑战之一.目前公有链中基于匿名认证和交易混淆的身份隐私保护方案由于缺乏监管又难于在行业应用中推广.借鉴门罗币中的身份隐私保护方案,引入监管方的角色,基于一次性地址加密和零知识证明设计了可监管的交易接收方身份隐私保护方案;结合可链接环签名和可撤销环签名设计了可链接可撤销环签名方案,以实现基于自主混淆的可监管交易发送方身份隐私保护方案.基于上述方案,系统在保护交易方身份隐私的同时,还支持监管方可离线恢复交易参与方的真实身份,从而达到“可控匿名”的监管目的.分析和测试结果表明,方案设计的算法运算时间均为毫秒级,可满足区块链非高频交易场景下的性能需求.

摘要：针对重装空投过程中,重型货物持续移动及瞬间离机严重影响载机的安全性等问题,提出了基于线性化反馈和滑模变结构控制相结合的控制律设计方法,利用非线性多输入多输出反馈线性化完成系统解耦线性化,在此基础上采用滑模变结构控制设计系统内环速度与俯仰姿态跟踪控制器,保证了系统鲁棒性,结合外环PID高度保持控制器完成整个飞行控制系统的设计.最后,仿真验证了该控制器鲁棒性强,且满足空投任务战技指标要求.

摘要：在信息安全的许多实际应用中往往需要同时提供私密性和认证性,通常采用加密模式和消息认证码的组合来实现这一目的,但这种实现方式须对同一消息分加密和认证两阶段进行处理,不仅密钥使用量大,而且效率低下.本文基于CBC加密模式设计了一种新的一阶段加密认证方案OXCBC,能够同时提供私密性和认证性,且仅使用一个密钥和一个Nonce,与同类型的加密认证方案相比具有较高的效率.在分组密码是强伪随机置换的假设下,证明了该方案的认证性.

摘要：介绍了反应堆重水系统管道安装的特点及主要安装步骤。结合系统设计、设备供应和现场管理等实际工作情况,分析了影响管道安装质量和进度的主要问题并提出以下解决办法:①以书面形式确定设计工作的接口划分并严格执行,使设计接口协调一致;②充分、彻底地做好设计审查、技术交底等安装准备工作;③系统设计应考虑系统冲洗、各试验检验项目、在役检查和设备维修的综合要求;④建立完善的文件发布和分发系统,按照最新的文件分发清单分发文件;⑤制定详尽的物项采购计划;⑥在物项采购合同中,应对采购物项的技术要求和质量保证要求作出规定;⑦在物项加工过程中,采用要求供方提供有关质量的客观证据、在供方驻厂监造、外部质保监查以及交货检验等措施对所购物项的质量进行控制;⑧制定一系列项目管理程序,明确职责分工、联络渠道等内容。

摘要：基于Hash函数的HMAC是一种应用最为广泛的消息认证码,但最近的研究指出HMAC不仅易受到相关密钥攻击,在多用户环境下也易受到攻击.为了避免这些问题,我们对HMAC进行了改进,基于Hash函数和分组密码设计了一种新的消息认证码HBMAC.在分组密码是伪随机置换和Hash函数所使用的压缩函数是伪随机函数的基本假设下,使用共享随机函数模型证明了HBMAC的安全性.同时,还提出了HBMAC和HMAC的算法实现,并基于典型数据对两种算法的性质和效率进行了分析.结果表明,与HMAC相比,HBMAC在安全性和效率上取得了更好的折衷.