摘要：Industrial development can enrich people＇s lives, but it also causes environmental pollution, which is an area of significant concern among governments, consumers, and companies. Governments formulate some environmental policies, which motivate industry by providing greater incentives for green product development. Consumers are classified into two groups - ordinary and green market segments - according to their environmental awareness and attitudes. In this regard, companies need to reconsider their primary product design strategies. By investigating the interactions among customers＇ preferences, firms＇ product strategies, and government subsidy policies, this paper presents a theoretical model for new product design strategies. After detailed theoretical analysis of such strategies, we found that to motivate firms to choose environmentally friendly product design strategies, governments should inaugurate effective subsidy policies. After simultaneously considering environmental issues and firms＇ benefits, we designed a subsidy policy. With such a policy, firms can change their primary product design strategies and develop both green and ordinary products, thereby increasing the firms＇ profits and improving the total environmental quality.

摘要：对肺炎链球菌双组份系统中的组氨酸激酶YycG进行同源模建,并分析其与底物ADP的相互作用,为寻找特异性的激酶抑制剂提供了理论依据。采用同源模建的方法构建YycG蛋白的三维结构,并用ProCheck、Profile_3D软件对此结构模型的合理性进行验证;用Autodock4.0软件将结构模型与ADP进行自动对接,分析二者之间的相互作用。序列比对结果显示肺炎链球菌YycG蛋白与Thermotoga maritima X-ray晶体结构序列的同一性达33%;YycG模建后的结构与模板能很好的叠合;在活性口袋处的保守的氨基酸残基Asn145、Asn149、Lys152以及口袋内部的疏水残基在结合、水解底物ADP的过程中发挥重要作用。组氨酸激酶YycG的模建合理,该结构模型可作为设计抗菌药的研究起点。

摘要：Objective] This study aimed to clone ovine activin receptor type llB （Ac-tRIIB） gene, construct the prokaryotic expression vector and express the target gene in vitro, thus providing basis for further function verification. [Method] The template cDNA which was reversely transcribed from total RNA of sheep liver tissue, was subjected to polymerase chain reaction （PCR） using specific primers of ActRIIB. The ful-length cDNA of ovine ActRIIB was obtained by pMD18-T cloning and sequencing for bioinformatics analysis. Ovine ActRIIB encoding sequence was subcloned into prokaryotic expression vector pET41a with restriction sites BamHl/Notl, and then transformed into BL21 （DE3）. The induced products by lPTG were analyzed with SDS-PAGE and Western Blot. [Result] The amplified ful-length cDNA of ovine Ac-tRllB gene was 1 564 bp in length （Genbank accession number： JX422071.1） with an open reading frame of 1 539 bp, encoding 512 amino acides. Ovine ActRllB shared the highest homology （99.6%） with bovine ActRllB. ActRllB had highly ho-mologous C-terminal domains and belonged to the TGFβ family. After prokaryotic expression, an approximately 92 kD His-tagged ActRllB recombinant protein was obtained, which was consistent with the excepted result. [Conclusion] Cloning and successful expression of ovine ActRIIB laid solid foundation for further investigation of its biological function.

摘要：为提升水凝胶的综合力学力学性能,设计并研究了一种全物理交联三重互穿网络水凝胶。以自由基乳液聚合法,获得疏水缔合交联的聚丙烯酰胺网络,水凝胶一步成型,依次通过冷冻-融化循环、浸没FeCl_(3)溶液分别形成微晶交联的聚乙烯醇网络及离子交联的海藻酸钠网络。获得P(AM-SMA)/PVA/SA三重互穿网络(HMITN)水凝胶。通过傅里叶变换红外对水凝胶结构进行了表征;通过电子万能试验机对水凝胶的力学性能进行了研究。结果表明,HMITN水凝胶具有良好的强度、韧性、抗疲劳及自恢复性能,强度0.45 MPa,断裂能2.48 MJ/m^(3),在200%应变下进行5次循环拉伸后仍然保持20.3%的韧性,室温下自恢复260 min后,韧性可恢复66.7%。

摘要：书籍装帧是书籍存在和视觉传递的重要形式，它是一门设计艺术，具有审美的、功能的、工艺的多元化本质，从外在形态上它具有整体性，从内涵上它是一门表现艺术。优秀的装帧设计都是充分发挥其各要素之间的关系，达到一种由表及里的完美。随着时代的发展，人们审美情趣的变化，书籍仅以功能形式的出现已经不能满足社会和市场的需要，人们不仅注重书的实用功能，而且日渐重视它的美观价值。因而，要发展我国的书籍装帧艺术首先要研究和探索现代书籍装帧艺术的特性。

摘要：物质生活水平的提升,促进人类衣食住行品质上的追求。对居住环境,除了最基本的居住功能外,开始注重视觉感受,室内建筑艺术设计表现形式趋于多样化,几何形态的应用,凸显艺术性和实用性特征,彰显了与众不同的设计魅力。

摘要：数字艺术表现形式是当下比较新潮的方式,在我国动画业崛起的时代,越来越被大众所喜爱。在各类动画中,三维动画带来的真实性最具魅力。本文旨在运用三维动画中艺术与技术结合来表现三维动画的真实性。探索如何通过三维制作表现动画真实性的美感。

摘要：分析了基于TBS建模设计过程中,从顶层基本骨架到子级基本骨架,再到零件产品信息逐层扩充的传递过程。分析了该过程中产品所具有的顶层基本骨架、子级骨架、零件三种信息状态,产品信息状态之间的信息传递具有串联、并联结构。分别针对产品信息状态转化的串联、并联、多路径等结构以及变型设计,提出通过计算转移概率来测度产品信息状态间的信息距离,为基于TBS自顶向下建模设计过程提供制造信息学方面的依据。

摘要：电能表用电流互感器的性能直接影响到电能表计量的准确度。针对目前电能表用电磁式电流互感器易饱和、测量范围较小等问题,提出了一种基于印制电路板(PCB)型电子式电流互感器的多功能电能表的设计方案。首先对PCB型空心线圈的结构及原理进行了分析,设计了空心线圈与计量芯片间的接口电路,并通过试验数据表明互感器具有良好的性能,然后详细阐述了以三相高精度计量芯片ADE7758和高性能、低功耗H8单片机为核心的电能表的硬件设计,在此基础上简要说明了电能表的软件设计思路。所设计的电能表计量准确度高,功耗低,具有很强的实用性。

摘要：当前,课程思政在高校教学改革中尤为重要,如何结合专业学科优势、提炼思政元素融入专业课教学全过程,真正实现教书育人,是课程思政的一个难点。本文通过作者多年专业教学实践,以“手风琴表演”课程为例,开展专业课课程思政教学设计方面的实践探索,与同行交流借鉴。