摘要：The intestinal epithelium is the main barrier to the oral delivery of poorly water-soluble drugs. Based on the specific transporters expressed on the apical membrane of the intestinal epithelium, novel polymer micelles targeting to the organic cation transporter 2（OCTN2） were constructed by combining carnitine conjugated poly（2-ethyl-2-oxazoline）-poly（D,L-lactide）（Car-PEOz-PLA） with monomethoxy poly（ethylene glycol）-poly（D,L-lactide）（mP EG-PLA）. The structure of the synthesized Car-PEOz-PLA was confirmed by -1H NMR, TLC and ammonium reineckate precipitation reaction, and the number-average molecular weight determined by GPC was 7260 g/mol with a low PDI of 1.44. Coumarin 6-loaded carnitine modified polymeric micelles prepared by film hydration method were characterized to have a nano-scaled size of about 31 nm in diameter, uniform spherical morphology, high drug loading content of 0.098%±0.03% and encapsulation efficiency of 92.67%±2.80%. Moreover, the carnitine-modified micelles exhibited the similar in vitro release behavior in SGF and SIF, and evidently enhanced intestinal absorption of poorly water-soluble agent. Therefore, the designed OCTN2-targeted micelles might have a promising potential for oral delivery of poorly water-soluble drugs.

摘要：In the present study, we designed and fabricated pH-sensitive polymeric micelles based on the conjugate of poly（2-ethyl-2-oxazoline）-poly（D,L-lactide）（PEOz-PLA） with doxorubicin（PEOz-PLA-imi-DOX） to efficiently inhibit tumor cell growth. Hence, PEOz-PLA-imi-DOX was successfully synthesized by connecting DOX to the hydrophobic end of pH-sensitive PEOz-PLA via acid cleavable benzoic imine linker and characterized by 1 H NMR spectrum and thin layer chromatography. The critical micelle concentration of PEOz-PLA-imi-DOX was determined to be（14.84±3.85） mg/L. The conjugate micelles（denoted as PP-DOX-PM） formed by PEOz-PLA-imi-DOX using film-hydration method were characterized to have a nano-scaled size of about 21 nm in diameter, and the drug loading content was 1.67%. PP-DOX-PM showed pH-dependent drug release behavior with gradually accelerated release of DOX with decrease of pH value, illustrating the micelles＇ distinguishing feature of endo/lysosomal pH from physiological pH by accelerating drug release. As anticipated, PP-DOX-PM maintained the cytotoxicity of DOX against MDA-MB-231 cells. Collectively, PP-DOX-PM might have great potential for effective suppression of tumor growth.

摘要：To ensure the delivery of antitumor drugs to tumor site and quick release in tumor cells, we designed and prepared pH-sensitive polymeric micelles by combining cationic ring-opening polymerization of 2-ethyl-2-oxazoline （EOz） with vitamin E succinate （VES）, and then encapsulating paclitaxel （PTX） into the micelles self-assembled by poly（2-ethyl-2-oxazoline）-vitamin E succinate （PEOz-VES）. The structure of the synthesized PEOz-VES was confirmed by ^1H NMR spectrum, and the molecular weight measured by GPC was 1212 g/mol. The pKa of PEOz-VES with a low critical micelle concentration of （5.84±0.02） mg/L was determined to be 6.01. The PTX-loaded PEOz-VES polymeric micelles prepared by film hydration method were characterized to have a nanoscaled size of about 30 nm in diameter, a positive Zeta potential of 4.86 mV and uniform spherical morphology by TEM observation. The drug loading content and encapsulation efficiency were （2.63±0.16）% and （84.1±3.38）%, respectively. The in vitro release behavior of PTX from PEOz-VES micelles in PBS displayed pH-dependent pattern and was gradually accelerated with decrease of pH value, implying that the micelles could distinguish endo/lysosomal pH and tumor extracellular pH from physiological pH by accelerating drug release. Therefore, the designed PEOz-VES micelles might have significant promise for anti-cancer drug delivery.

摘要：凯瑟琳·菲茨莫里斯声音方法作为基于人体医学科学的本体属性,要求其在科学理性特质上与自然主义方法论保持一致。该方法以灵活借用解构主义理论,着重于消解结构再重新结构的"解构—重构"理念,彰显着其结构主义科学理性的思维气质。该方法在凸显演员创造主体性的同时,立足现实意义的运用价值并极富浪漫主义色彩地探寻着被分析者与分析者关系的系统化,表征着一种方法派观念。该方法所蕴含着的对艺术美的主体性创造及创造主体自由性的强调正契合着康德美学自然观的意涵。

摘要：表面活性物质的表面张力等温线测定是大学物理化学实验的重要内容。随着磷脂类表面活性剂的广泛应用,有必要在大学化学中更新经典表面张力等温线测定的实验内容,并使学生掌握先进的测量方法。以二硬脂酰基磷脂酰胆碱(DSPC)和二棕榈酰基磷脂酰胆碱(DPPC)为研究对象,应用界面张力仪测定了两种溶液表面张力等温线,计算了其表面超量和分子横截面积,并设计了拓展性实验内容,对于培养学生分析问题和解决问题的能力具有积极的意义。

摘要：由多个投影仪拼接显示的自由立体显示系统有很大的显示区域,能够产生更好的立体显示效果.针对可伸缩高分辨率多投影仪自由立体显示的系统特性和屏幕特性,通过分析各种几何校正和亮度校正算法,采用二次几何校正方法解决了自由立体显示中斜投影造成的部分特征点不在屏幕上的问题;提出了基于GPU的亮度自适应多模板校正方法,并通过GPU程序将几何校正和亮度校正合并为一个渲染过程,显著地提高了几何校正和亮度校正的速度.实验结果证明,采用文中方法能够有效地解决自由立体投影显示系统中的几何校正和亮度校正问题,且校正速度快、显示效果好.

摘要：脑神经损伤及认知衰退人群存在运动能力下降、言语功能退化、跌倒风险增大等问题,目前的功能康复机器人主要关注于被动训练,运动康复效果不佳.设计了一款新型认知运动康复机器人,开发其"感知—认知—运动"多模态智能感知交互功能.采用Pepper机器人搭建了一个集认知、运动康复训练为一体的康复机器人系统:首先,设计了多模态的智能感知及交互系统,实现了与患者的视听触觉交互和言语训练;其次,利用机器人声呐、激光和视觉实现实时地图构建及人体追踪;最后,将深度学习方法应用到机器人上,实现了基于机器人视觉的人体姿态识别,为下一步通过视频图像判别人体稳定性提供了可行性方法.实验结果显示设计的康复机器人系统在认知运动协同康复训练过程中具有正作用,该研究初步证明了智能机器人在医疗辅助康复训练上的可行性,被期望未来用于居家及临床上的认知运动协同康复.

摘要：目的:分析蛋白EgG1Y162-2通过不同长度接头序列连接后形成的肽段四聚体蛋白EgG1Y162-2(4)的氨基酸序列特征,明确其理化性质及三级结构,预测并对比经不同长度柔性接头连接后其优势抗原表位变化,为增强重组疫苗免疫原性选取最理想的linker序列。方法:利用生物信息学方法选择GSGGSG、GGGGSGGG和GSGGSGGGSGGSGGG 3种linker序列进行连接设计EgG1Y162-2(4)重组蛋白疫苗。在线软件ProtParam分析其理化性质;SOPMA在线数据库对设计有不同linker序列的重组蛋白二级结构进行预测分析;I-TASSER在线软件预测所设计重组疫苗的三级结构,并使用SYFPEITHI、IEDB等软件对其T/B细胞抗原表位进行预测。结果:经预测通过GSGGSG、GGGGSGGG、GSGGSGGGSGGSGGG 3种linker序列连接的EgG1Y162-2(4)均是稳定蛋白且具有亲水性并得到其二级结构特点。设计的重组疫苗EgG1Y162-2(4)通过生物信息学方法预测得知通过GSGGSG连接的EgG1Y162-2(4)中α螺旋占8.50%,β-转角占16.67%,无规则卷曲约占40.48%,延伸链约占34.35%,该蛋白有8个T/B联合表位,前后串联的EgG1Y162-2蛋白不仅能够正常折叠,且与EgG1Y162-2蛋白相比T/B抗原表位未发生偏移。三级结构显示通过linker序列GSGGSG串联的4个蛋白EgG1Y162-2均能正常表达,而通过GGGGSGGG、GSGGSGGGSGGSGGG串联的蛋白EgG1Y162-2(4)表位发生了一定程度右移。结论:利用GSGGSG 6个氨基酸连接EgG1Y162-2蛋白构成的EgG1Y162-2(4)的T/B联合表位高度重合,表位未发生迁移说明蛋白EgG1Y162-2(4)通过这种方式连接后几乎未对EgG1Y162-2蛋白优势表位造成影响,并通过重复蛋白表位数增强疫苗免疫应答效果,制备有效的棘球蚴病表位疫苗。

摘要：20世纪60年代,法国哲学家德里达翻译并挪用德国哲学家海德格尔的“解构”一词,从法语词典《利特雷》里拎出了他的“解构”主义观,让主宰了西方哲学两千多年的逻各斯中心主义传统思想受到了前所未有的挑战。“在场的形而上学”中的“在场”性遭到质疑;解构主义反其道而行,从“结构”内部肢解、拆分了已占据统领地位多年的权威理论,以一种新的姿态带来了另一种理解世界的视角。从德里达的解构主义出发,在戏剧表演研究中借用解构主义中“解构”“分拆(析)”“延异”等观念分析演员现阶段呈现的表演议题,可以对演员的二度创作过程进行剖析和反省,从而实现演员二度创作的“重构”。

摘要：身体声音训练融合了身体的生理性、发声、情感表达等元素训练。现阶段,以身体声音为研究对象的学术成果较少,同时,要在这样的学术背景之下做到身体声音训练的民族化,是相关专业教师亟待解决的问题。本文从声音训练以及教学民族化的基本现状出发,以西藏班学生的性格特征作为切入点,根据西藏班学生文化的独特性、性格的双重性等方面进行阐述,并在此基础上提出对于声音教学和民族化这一问题的创新思考,在教育过程中实现两者的灵活结合,以期能够对身体声音训练课程起到一定的推动作用。