摘要：The presence of anti-p53 antibody in serum is a biomarker for cancer. However, its high sensitivity detection is still an issue in cancer diagnosis. To tackle this challenge, we used fd phage, a human-safe bacteria-specific virus nanofiber that can be mass-produced by infecting host bacteria in an error-free manner, and genetically engineered it to display a peptide capable of recognizing and capturing anti-p53 antibody on its side wall. We employed the resultant phage nanofibers as a capture probe to develop a modified version of the enzyme- linked immunosorbent assay （ELISA） method, termed phage-ELISA. We compared it to the traditional ELISA method for the detection of anti-p53 antibody, p53-ELISA, which uses recombinant wild-type p53 protein to capture anti-p53 antibody. We applied phage-ELISA to detect anti-p53 antibody in an experimental group of 316 patients with various types of malignant tumors. We found that a detection rate of 17.7% （56 positive cases） was achieved by phage-ELISA, which was comparable to the detection rate of 20.6% for p53-ELISA （65 positive cases）. However, when both phage and p53 were combined to form antibody-capturing probes for phage/p53-ELISA, a detection rate of 30.4% （96 positive cases） was achieved. Our work showed that owing to the combined capture of the anti-p53 antibody by both phage nanofibers and p53, the phage/p53-ELISA achieved the highest diagnostic accuracy and detection efficiency for the anti-p53 antibody in patients with various types of cancers. Our work suggests that a combination of nanofibers and antigens, both of which capture antibody, could lead to increased detection sensitivity, which is useful for applications in the life sciences, clinical medicine, and environmental sciences.

摘要：Candida albicans （C. albicans） infection has a high mortality rate in immunocompromised patients. Owing to the inefficiency of the current diagnostic system and the absence of licensed vaccines against candidiasis, the prevention of C. albicans infection remains a challenge. C. albicans infection can be evaluated and prevented by the anti-secreted aspartyl proteinase 2 antibody （anti-Sap2 IgG） and Hsp90 antibody （anti-Hsp90 IgG）. In this study, to explore a new agent for the improvement of the diagnosis and the prevention of C. albicans infection, an engineered fd bacteriophage, which is considered a human-safe virus nanofiber, was designed and prepared with two epitopes that could induce and capture anti-Sap2 IgG and anti-Hsp90 IgG. The dual-display phage was employed as a novel capture probe to develop a new enzyme-linked immunosorbent assay （ELISA） method, which significantly improved the detection rate compared with those of the ELISA in which recombinant protein Sap2 was used as coating antigen to capture the spedfic antibodies （rSap2-ELISA） and the ELISA in which recombinant protein Hsp90 was used as coating antigen to capture the specific antibodies （rHsp90-ELISA）. In addition, the nanofibers acted as a potential vaccine to immunize mice, as well as recombinant proteins, more efficiently mediated humoral and cellular immune responses, decreased levels of C. albicans colonization, and increased the survival rates in C. albicans-infected mice. Therefore, the phage dual-display nanofiber has been shown to be a powerful bifunctional agent for protection against and sensitive detection of clinical infections, which has the potential to be widely used in the life sciences, clinical medicine, and environmental sciences.

摘要：The existing wireless power transfer(WPT)systems for gastrointestinal capsule robot have the prob-lems of small coupling coefficient and low power transmission efficiency(PTE).The reasons are due to the long distance between the transmitting coil and the receiving coil and the large difference in size.A new type of WPT system is designed,which uses three sets of small coil pairs to form a power supply unit(PSU),and utilizes multiple PSUs to form a multi-coil WPT *** with single-coil system,the multi-coil system can achieve higher power utilization by switching between PSUs,instead of opening all *** Maxwell is used to perform finite element modeling on the PSU,analyzing the characteristics of the transmitting magnetic *** results of the experiment show that when the distance between the small coil pairs in the PSU is 180mm,the magnetic field has relatively good uniformity,and the magnetic strength change relative to the center point is less than 5%.The average received power of the system is greater than 800mW,and the PTE is up to 5.1%.

摘要：进行了6个国产6063-T5铝合金试样拉伸试验,测得其应力-应变关系曲线,并与Ramberg-Osgood模型进行了对比。结果表明,Ramberg-Osgood模型可较准确描述国产6063-T5铝合金材料的力学性能。通过对4种截面类型共10根闭口加劲薄壁国产6063-T5铝合金试件进行轴压试验,获得了各试件荷载-轴向位移曲线、荷载-板件平面外变形曲线以及荷载-应变曲线,得到其承载力和破坏形态。结果表明,各试件均发生局部屈曲与塑性屈服耦合破坏。采用有限元分析方法对试件进行模拟,模拟结果与试验结果的对比表明,所建立的有限元模型可以较准确预测铝合金试件的承载力和破坏形态。采用现行美国、欧洲、中国的铝合金结构设计规范、美国冷弯薄壁钢结构设计规范及直接强度法对所研究试件的承载力进行计算,计算结果与试验结果的对比表明,各规范设计方法基本适用于闭口加劲薄壁铝合金轴压构件局部屈曲设计。直接强度法计算结果与实测承载力比值约为1.20,表明目前直接强度法高估了闭口加劲薄壁截面铝合金轴压构件承载力,因此需对其修正才可用于该类闭口加劲薄壁截面铝合金轴压构件的承载分析。

摘要：以聚己内酰胺(PA6)和聚对苯二甲酸乙二醇酯(PET)粒料为原料,利用高压毛细管流变仪研究了材料体系的基本流变行为和分相条件,进而通过双螺杆挤出机制得了具有包覆结构的PA6/PET共混物。通过调节挤出过程中的剪切速率研究了两种组分的黏度比对最终制品中包覆结构的影响。结果表明,PET最终能够迁移至挤出物表层对PA6相产生包覆;在两组分界面处,PET的聚集程度沿半径方向呈梯度变化,这一结构为最终材料提供了良好的界面性能;两组分间的熔体黏度是否相互匹配是包覆结构形成的前提条件;存在一最优的剪切速率范围及黏度比范围,在该加工范围内得到的最终材料的包覆结构较为完善并且耐腐蚀性能较为优异。

摘要：药理学作为一门基础性医学类学科,是学生掌握基础药理学知识的关键,有关实验课程更是教学过程中的关键。在以往药理学课程教学过程中,实验教学大多数都是验证性方面的实验,实验过程学生依靠既定的实验步骤方法来进行,整个实验过程较为被动,学生的思考机会较少。而设计性实验则采取了更加科学的方式,突出了学生的主体性地位,以科学的手段来对实验技术和方法进行重新设计,整个实验的探索性得到了大大的提高,对学生自主研究、创新能力和动手能力的培养具有重要意义。

摘要：对NCBI文库公布的120条不同亚型禽流感病毒的M基因序列进行分析,筛选得到一处高保守片段并进行重组酶介导等温核酸扩增(recombinase-aided amplification,RAA)引物和crRNA的设计。待测样品先进行RAA核酸扩增,再将扩增产物转移至有规律成簇间隔短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)/Cas13a检测体系中,通过监测该体系中荧光值的变化情况进行结果判定。使用梯度稀释(10^(6)~10^(0)拷贝/μL)的标准阳性质粒以及其他7种禽病病毒验证方法的灵敏度和特异性,并利用本试验所建方法和国标荧光RT-PCR方法对50份临床样品(21份阳性样品、29份阴性样品)进行检测以评价方法的检测性能。结果显示,本试验建立的检测方法灵敏度为10^(2)拷贝/μL,较普通RAA方法灵敏度提高了2个数量级;能特异性检出AIV,不与NDV、IBV、ILTV、IBDV、ALV、AMPV、AAV-1发生交叉反应;与国标荧光RT-PCR方法相比,该方法检测的特异性、准确性和一致性分别为100%、95.24%和98.00%。结果表明,本试验建立了检测AIV通用型的RAA-CRISPR/Cas13a快速诊断技术,其在37℃条件下60 min内即可完成检测,具有快速、灵敏、特异等优势,为AIV的现场快速检测提供了手段。