摘要：The electrospinning process was applied to fabricate the nanofibers of biodegradable poly(ε-caprolactone)(PCL) in which different contents of multiwalled carbon nanotubes(MWCNTs) were embedded. Afterward,the electrospun nanofibers were successfully decorated with shish-kebab structure via a self-induced crystallization technique. The topographical features and the mechanical properties of the composite scaffolds were characterized,and the biocompatibility of the material was assessed by using human osteogenic sarcoma osteoblasts(MG-63 cells). The carbon nanotube(CNT) concentration is found to affect the fiber diameter and mechanical properties of electrospun nanofibers and the periodic distance of the shish-kebab architecture. Cellular attachment and proliferation assays reveal that 0.5 wt% CNT-embedded PCL scaffold shows enhanced biocompatibility with MG-63 cells than their counterparts made of neat PCL, and the collagen-like nanotopology provided by the shish-kebab structure further facilitates the cell adhesion and proliferation. The superior interactions between cells and scaffolds demonstrate that the shish-kebab-structured CNTs/PCL nanofibers may be promising candidate for tissue engineering scaffold application.

摘要：钢材打包是钢材生产的重要环节,锁扣的力学性能对钢材打包质量起关键作用。本文研究了一种新型的免扣无反扣钢带打包方式进行研究。通过分析扣的成形原理,得到关键尺寸,用三维建模软件UG对锁扣建模,分析免扣无反扣抗拉模式,利用Abaqus软件对扣进行仿真推演,得到强度效率、应力极限。最终得到结论:免扣无反扣打包方式的强度效率达到77.8%,高于常用打包方式。

摘要：The organized alignment of cells in various tissues plays a significant role in the maintenance of specific *** induce such an alignment,ideal scaffolds should simulate the characteristics and morphologies of natural *** structures that guide cell orientation are used to facilitate tissue regeneration and *** here review how various aligned structures are fabricated,including aligned electrospun nanofibers,aligned porous or channeled structures,micropatterns and combinations thereof,and their application in nerve,skeletal muscle,tendon,and tubular dentin *** future use of aligned structures in tissue engineering is also discussed.

摘要：With the potential application of Energy Recovery Linac (ERL), the superconducting (SC) cavities were developed to deliver much higher current than before. Nowadays, the current of the international SC accelerator designed has already exceeded 100 ma. This paper presents the design of a new 500 MHz 5-cell SC cavity (SINAP 5-cell cavity), in which the parameters r/Q= 515.5 of the fundamental mode and the geometry factor G=275.8 are under an acceptable Radio Frequency (RF) field level (B peak /E acc =4.31 mT/MV/m and E peak /E acc =2.48). This design employs a larger beam pipe to propagate the Higher Order Modes (HOMs) out of the cavity and increases the damping efficiently for the dangerous HOMs. By simulation technique, it has been found that almost all the dangerous HOMs (including TE 111 , TM 110 , and TM 011 ) can be propagated into the beam pipe and are absorbed by ferrite absorbers, when the beam pile is enlarged. Finally, the loss factor for the new 5-cell cavity is also calculated.

摘要：Seven 2,6-disubstituted N-(2-phenoxy)ethyl imidazo[1,2-a]pyridine-3-carboxamide series containing various amine moieties were designed and synthesized as new anti-TB *** of them show excellent in vitro activity against both drug-sensitive MTB strain H37Rv and two MDR-MTB clinical isolates(MIC:<0.002-0.030 μg/mL).Compounds 2f,5e and 5g display acceptable safety and pharmacokinetic profiles,opening a new direction for further development.

摘要：目的建立一种基于微流控芯片平台的可同时鉴别沙眼衣原体(Chlamydia trachomatis,CT)、淋球菌(Neisseria gonorrhoeae,NG)、解脲脲原体(Ureaplasma urealyticum,UU)的现场化全自动检测方法。方法选取CT的隐蔽性质粒、NG的porA基因和UU的16SrRNA中的保守序列作为检测靶标,设计特异性引物和Taqman探针;分别制备CT、NG、UU三种病原体的阳性克隆菌株进行灵敏度初步测试。开发基于全自动检测设备的一体化微流控芯片,实现“样本进-结果出”的核酸提取纯化及实时荧光PCR全过程。使用经过数字PCR定量的CT、NG、UU三种病原体的液体室内质控品对全自动检测方法的灵敏度、特异性和重复性进行评价。用建立的全自动检测方法与已上市的多重实时荧光PCR法检测试剂盒同时检测160例临床样本,评价其一致性。结果阳性克隆菌株经琼脂糖凝胶电泳和测序鉴定,构建正确。建立的全自动检测方法检测CT、NG和UU的检出限均可达到1×10^(2) copies/test,检测范围1×10^(2)~1×10^(7) copies/test;该方法特异性好,不受单纯疱疹病毒Ⅰ型/Ⅱ型、腺病毒、肠道病毒71型、肺炎衣原体、肺炎支原体以及人免疫缺陷病毒Ⅰ型的干扰;该方法重复性好,变异系数均0.05);全自动检测方法与对比试剂盒检测CT、NG和UU结果的阳性符合率分别为96.2%、94.4%和93.4%,阴性符合率均为100%,总符合率分别为99.4%、99.4%和97.5%。结论建立的现场化全自动检测方法具有良好的灵敏度、特异性和重复性,该方法自动化程度高,检测时间短,操作简便、快速,不受实验室场地限制,适合于CT、NG、UU的临床快速诊断。

摘要：为了探讨“互联网+”时代如何合理、适当地设计并运用线上教学资源构建新型课堂,使线上线下混合式教学达成既定目标,以南京中医药大学中医学专业2020级本科生为研究对象,以药理学中“呼吸系统药”章节为例,从课前、课中、课后3个阶段探索线上线下混合式教学模式在教学中的应用。结果发现线上与线下课堂的有效融合解决了传统课堂教学形式枯燥单一、学生主体地位易被忽略、师生缺乏互动、教学内容不可重温及单纯线上教学学生自觉性差等问题,提高了教学效率、提升了教学活力,较好地完成了教学目标和教学任务。

摘要：Dear Editor, Artificial expression systems are crucial to functional genomics studies, constructing synthetic genetic circuits, and bioengineering (Werner & Gossen, 2014). They are usually achieved by cloning the coding sequence (CDS) of a target gene into a vector containing natural or engineered regulatory elements. However, this strategy is limited when target genes are hard to clone, for example when the CDS is too long, or expression level is too low.

摘要：TiO2 pigments are typically coated with inert layers to suppress the photocatalytic activity and improve the weatherability. However, the traditional inert layers have a lower refractive index compared to TiO2, and therefore reduce the lightening power of TiO2. In the present work, a uniform, amorphous, 2.9-nm-thick TiO2 protective layer was deposited onto the surface of anatase TiO2 pigments according to pulsed chemical vapor deposition at room temperature, with Ti Cl4 as titanium precursor. Amorphous TiO2 coating layers exhibited poor photocatalytic activity, leading to a boosted weatherability. Similarly, this coating method is also effective for TiO2 coating with amorphous SiO2 and SnO2 layers. However, the lightening power of amorphous TiO2 layer is higher than those of amorphous SiO2 and SnO2 layers. According to the measurements of photoluminescence lifetime, surface photocurrent density, charge-transfer resistance, and electron spin resonance spectroscopy, it is revealed that the amorphous layer can prevent the migration of photogenerated electrons and holes onto the surface, decreasing the densities of surface electron and hole, and thereby suppress the photocatalytic activity.

摘要：背景:颈椎失稳是中青年人群常见骨科疾病,是颈椎病早期表现,对患者生活质量影响较大。因此,早期诊断颈椎失稳以实施早期干预具有积极的临床意义和社会意义。目的:基于机器学习构建中青年人群颈椎失稳临床预测模型,在进行X射线片检查之前,实现中青年人群颈椎失稳早期筛查。方法:2022年9月至2023年10月通过招募广告和中国中医科学院望京医院脊柱科门诊招募155例中青年颈椎失稳受试者和88例非颈椎失稳受试者作为研究对象,基于调查问卷形式现场采集受试者一般信息、生活工作习惯、不适症状、颈痛目测类比评分、颈椎功能障碍指数、健康调查简表(SF-36)等信息,并将以上信息作为预测因子,经筛选后,以支持向量机、轻量的梯度提升机、随机森林、逻辑回归分析、自适应提升算法、极致梯度提升分类器6种机器学习算法采用十折交叉验证方法进行模型训练,构建颈椎失稳临床预测模型,以曲线下面积为主要评价指标。将预测因子进行单因素分析,并采用SHAP方法对预测因子的重要性进行排序,以相关性热力图展现预测因子之间及其与颈椎失稳之间的线性相关程度。结果与结论:①6种机器学习模型中,选用随机森林模型为最终预测模型,包含年龄、体质量指数、颈围/颈长、颈痛目测类比评分、颈椎功能障碍指数、SF-36健康状况量表躯体疼痛、总体健康、生命活力、精神健康评分9个预测因子,曲线下面积=0.7254,具有良好的预测能力,可作为早期筛查中青年人群颈椎失稳的参考工具;②两组受试者的年龄、颈痛目测类比评分、颈椎功能障碍指数、SF-36健康状况量表躯体疼痛、总体健康、生命活力评分相比差异有显著性意义(P<0.05);③预测因子重要性排序为年龄、颈椎功能障碍指数、颈痛目测类比评分、总体健康、体质量指数、生命活力、躯体疼痛、颈围/颈长、精神健康,其中年龄、颈痛目测类比评分、颈椎功能障碍指数与颈椎失稳呈正相关,总体健康、体质量指数、生命活力、躯体疼痛、颈围/颈长、精神健康与颈椎失稳呈负相关。