摘要：We hypothesized that neuroprotective agents targeting various pathways involved in cerebral ischemia/reperfusion(I/R)injury might be superior to that targeting single ***,we prepared a fusion protein(B-I)by combining anti-apoptotic Bcl-x L(B)and anti-inflammatory IL-10(I).B-I could cross blood brain barrier by its N-terminal TAT domain,and be cleaved into separate B and I by Caspase-1.B-I treatment significantly reduced the cerebral infarct volume,better than B or I treatment alone,and equivalent to B and I treatment(B+I).Treatment with B or B-I significantly attenuated I/R-induced neuronal apoptosis as shown by the decrease in apoptotic rate and the inhibition of caspase-3 ***,all recombinant proteins,especially B-I,remarkably attenuated I/R-induced up-regulation of TNF-α.These results suggested that fusion protein B-I inhibiting both inflammation and apoptosis provided better neuroprotective effects than inhibiting either one *** study suggested that multiple pathways targeting brain I-R injury could enhance the neuroprotective effect,and it provided a new idea for the study of neuroprotective drugs for ischemic stroke.

摘要：We hypothesized that thrombolysis in combination with neuroprotection might have better therapeutic effects for ischemic stroke compared with thrombolysis *** order to verify such hypothesis,we designed a protein TBN by fusing NR6and BH4,which possibly had dual functions of thrombolysis and ***6was obtained by introducing two RGD motifs to thrombolytic protein AcAP5to target ***4is the key domain of anti-apoptotic protein Bcl-xL. The DNA fragments encoding TBN and NR6were synthesized and cloned into pET30a and pET16b vectors,*** proteins were expressed in ***21(DE3),mainly in the form of inclusion *** His-tag,NR6was purified by nickel affinity chromatography,while TBN was purified by ion exchange *** proteins were refolded by dialysis *** thrombolytic activity of both proteins was evaluated by the rat arteriovenous bypass *** NR6and TBN significantly reduced thrombus weight at higher dose (24nmol/kg),TBN showed similar effect to *** results suggested that NR6was a thrombolytic protein,and fusion protein TBN reserved the thrombolytic activation of *** effects of both proteins were also evaluated in thromboembolic middle cerebral artery occlusion (eMCAO)in *** exhibited better effect on reducing infarction volume and inhibiting apoptosis of cells than NR6,indicating that the introduction of BH4increased the protective effect of *** hemorrhagic side effects of the two proteins were evaluated by tail bleeding in mice,and it was found that NR6and TBN showed shorter bleeding time compared with *** conclusion,we designed and prepared the two novel proteins,and testified that they *** thrombolytic effect and protective effect on cerebral IR *** protective effect of TBN was more potent than *** bleeding side reaction might be weaker than *** results suggested that these two novel proteins deserved to be further investigated as new thro

摘要：文章统计呼伦贝尔市2019—2023年草原有害生物发生情况,结果表明:呼伦贝尔市草原有害生物危害面积946.9万公顷,严重危害面积475.4万公顷,防治面积489.8万公顷。其中:草原鼠害危害面积863.1万公顷,严重危害面积440.7万公顷,防治面积459.8万公顷;草原虫害危害面积75.9万公顷,严重危害面积31.7万公顷,防治面积28.5万公顷;毒害草危害面积7.9万公顷,严重危害面积3.0万公顷,防治面积1.5万公顷。2024年全市鼠害预计危害面积45.2万公顷,与2023年相比呈下降趋势;草原虫害预计危害面积16.9万公顷,与2023年相比呈上升趋势。在此基础上,提出持续加大草原有害生物防治投入力度、大范围使用生物药剂防治草原有害生物、增加绿色防控比例等对策建议。

摘要：Recombinant tissue plasminogen activator （rPA） has been used as a thrombolytic agent. However, considerable improvements have been done to prolong its plasma half-life （tl/2） and reduce its side effects, such as intracranial hemorrhage. Based on these improvements, a mutant ofrPA, mrPA, was designed by mutating its PAI-1 binding site to extend its tl/2. Furthermore, a fusion protein conjugating mrPA with NR3 was designed, which was a rAcAp5 mutant with a platelet GPIIb/IIIa-binding RGD motif, to enhance the ability of targeted-thrombus and thrombolysis. The synthesized DNA sequences coding the two proteins were amplified by PCR, cloned into pET30a to construct recombinant plasmids pET30a-mrPA and pET30a-mrPA-NR3, and transformed into E. coli BL21 （DE3）. The two proteins were expressed in inclusion bodies induced by isopropy113-D-1-thiogalactopyranoside. After purified to qualified purity using one-step Ni affinity chromatography, the denatured proteins were refolded by dialysis. Their thrombolytic effects in vitro and in vivo were evaluated. In vitro 3.5 and 7 pmol/L of mrPA significantly reduced thrombus weight; 1.75, 3.5 and 7 ~tmol/L of mrPA-NR3 also significantly reduced the thrombus weight, and mrPA-NR3 displayed stronger thrombolytic effects than mrPA at 7 lamol/L. In vivo both mrPA and mrPA-NR3 showed significantly thrombolytic effect at 60-240 ktmol/kg in thrombolytic model of inferior vena cava. Importantly, mrPA-NR3 exhibited more potent thrombolytic effect than both mrPA and rhM-tPA （positive control） at 240 ***/kg. In addition, these two novel proteins did not increase bleeding time while they exerted thrombolytic effect. In conclusion, we engineered two novel proteins and proved that fusion protein had better thrombolytic effect than non-fusion protein, and the results suggest that dual thrombolytic mechanism or thrombus-target potentiated the thrombolytic effect ofrPA and alleviated hemorrhage side reaction. This study may shed light on the developme

摘要：通过调研地面雷达的腐蚀情况,对发现的腐蚀点采用统计分析方法,全面系统地介绍了地面雷达的腐蚀,并针对腐蚀问题和腐蚀规律,提出了腐蚀控制的对策。在腐蚀现状调研分析中,重点介绍了雷达的腐蚀形态、制件暴露环境对腐蚀的影响、腐蚀主要原因及产生阶段;在腐蚀控制的对策分析中,重点阐述了环境控制设计、密封设计和遮蔽设计的要求及方法。通过数据分析,发现腐蚀规律,制定了结合雷达产品的特点和研发流程进行科学管理的方法体系,提出了树立从方案设计、工程设计到生产制造和使用维护等各阶段进行全方位、全过程和全寿命周期腐蚀综合控制的思想,从顶层完善了雷达产品的腐蚀控制理论体系。

摘要：以黄陵一号煤矿为例,开展了基于大数据的煤矿预警平台总体方案、各分系统及软件等方面设计研究,并对系统进行了实际应用效果测试。测试结果表明,所设计的预警平台运行正常,数据报警性能及准确性较好,大幅减少了设备故障率,缩短了故障排除时间,提高了煤矿的智能化水平。

摘要：研究张北防护林草本植物群落特征及其对林分密度的响应,对我国三北防护林的抚育、营建与可持续经营提供理论依据。本研究实地调查了张北防护林的草本植物群落平均高度、盖度、生物量、群落物种数以及林分密度,利用单因素方差分析及线性回归拟合曲线分析,探究张北防护林草本植物群落特征及其与林分密度的关系。结果表明:张北防护林下草本植物群落平均高度、盖度、生物量及群落物种数量在各样地内存在显著性差异,其中草本植物群落生物量变异系数最大,变化范围为3.03~103.1 g,草本植物群落物种数变异系数最小,变化范围为6~15;草本植物群落平均高度、草本植物群落盖度及草本植物群落生物量随林分密度升高均呈先增后减趋势,草本植物群落平均高度的最适林分密度范围为1 100~2 200 n/hm^2,草本植物群落盖度的最适林分密度范围为800~1 800 n/hm^2,草本植物群落生物量的最适林分密度范围为1 500~2 400 n/hm^2;草本植物群落物种数随林分密度升高呈逐渐降低趋势,草本植物群落物种数的最适林分密度范围为500~1 200 n/hm^2;综上考虑张北防护林林下草本植物群落特征,张北地区防护林最适林分密度应该在800~2 000 n/hm^2之间。适当调控林分密度,有利于提高林下草本植物群落的高度、盖度、生物量及物种数,将有利于张北防护林的更好经营和抚育。

摘要：人是生产力中最活跃的因素,在导致事故发生的种种原因中,人的不安全因素是重要的核心原因,为了搞好安全生产,防止事故发生,必须及时矫正各种不良心理,消除不安全行为。因此,以南方电网超高压输电公司为例,结合企业安全生产工作,对人的不安全行为进行研究,建立不安全行为标准,构建不安全行为闭环管控体系,并进行试点应用及总结。通过对人的不安全行为闭环管控研究,持续提升了安全生产水平,控制一切风险,消除一切隐患,预防一切事故。

摘要：为开发高性能微型可移动电源系统,设计并实验测试了一种介观尺度燃烧驱动的温差发电机(micro/mesoscale combustion powered thermoelectric generator,MCP-TEG)。MCP-TEG采用多台阶稳燃技术、烟气逆流换热技术和错列肋柱(Pin-fin)强化传热技术,同时将燃料混合器、预热器、燃烧器、稳燃器、集热器和均热器一体化集成。实验结果发现,设计的MCP-TEG的发电功率为29.6W,高于目前国内外的报道值,对应的发电效率为2.84%。此外,论文详细研究了MCP-TEG的输入功率、化学当量比、冷端散热和保温设计对发电性能的影响。实验发现存在一个最佳的化学当量比,使得MCP-TEG的发电功率最大。对于水冷型MCP-TEG,增加冷端散热并不一定能够获得更大的净发电功率。燃烧器的外壁面保温特性对发电性能有重要的影响,对于当前的MCP-TEG,燃烧器外壁面增加一层3 mm的保温层后,发电功率和发电效率均比不保温条件下的结果增加了4.6%。

摘要：为了保证沙河渡槽工程质量与安全可靠性,针对工程布置、结构选型与设计以及施工开展了一系列技术研究与试验。主要介绍沙河渡槽选址选线、渡槽总体结构选型与布置、水头优化分配、水力学模型试验、结构分析研究、槽身架槽机施工技术方案研究、预制槽蒸养温控仿真研究、原型槽充水与止水试验、预应力张拉试验、桩基静载试验等技术研究与试验成果。对于完善大流量预应力渡槽的设计理论,以及推动大型渡槽机械化施工具有重要的推广价值。