摘要：Advanced oxidation processes(AOPs) have been applied to address multiple environmental concerns including antibiotic resistance genes(ARGs). ARGs have shown an increasing threat to human health,and they are either harbored by antibiotic-resistant bacteria(ARB) or free in the ***, the control of ARGs has been substantially limited by their low concentration and the limited knowledge about their interfacial behavior. Herein, a novel AOP catalyst, Ag/TiO_(2)/graphene oxide(GO),combined with a polyvinylidene fluoride(PVDF) ultrafiltration membrane was designed with a synergistic interfacial adsorption and oxidation function to inactivate ARGs with high efficiency in both model solutions and in secondary wastewater effluent, especially when the residue concentration was *** analysis showed that the mineralization of bases and phosphodiesters mainly caused the inactivation of ARGs. Moreover, the interfacial adsorption and oxidation processes of ARGs were studied at the molecular level. The results showed that GO was rich in sp^(2) backbones and functional oxygen groups,which efficiently captured and enriched the ARGs via p-p interactions and hydrogen bonds. Therefore,the photogenerated active oxygen species attack the ARGs by partially overcoming the kinetic problems in this process. The Ag/Ti O2/GO catalyst was further combined with a PVDF membrane to test its potential in wastewater treatment applications. This work offers an efficient method and a corresponding material for the inactivation and mineralization of intra/extracellular ARGs. Moreover, the molecularlevel understanding of ARG behaviors on a solid–liquid interface will inspire further control strategies of ARGs in the future.

摘要：部分天然水体中存在大量病原菌,严重威胁人类健康,并引起生态系统失衡,亟需有效的技术手段进行杀灭.光催化技术已广泛应用于水体环境污染物的处理,然而,现有光催化剂存在波长响应范围小或光生电子-空穴易复合等问题.因此,我们设计出一种以WO_(3)为基底的光催化剂,通过3%Ag元素掺杂提升了电子-空穴分离效率并抑制其复合,从而高效灭活病原菌.具体而言,本文探究在可见光下,Ag元素掺杂的以WO_(3)为基底的光催化剂对大肠杆菌(***)的灭活性能.结果表明,Ag以3%质量比掺杂WO_(3)(AgWO_(3)-3)具有最佳杀菌效率,光照40 min后,大肠杆菌密度可由10^(7.23)CFU·mL^(-1)降低至10^(2.30)CFU·mL^(-1).后进一步分析了AgWO_(3)-3在不同浓度腐植酸、p H值和盐度条件下对***的灭活作用,结果显示Ag WO_(3)-3在光催化系统均促进杀灭***的性能.进一步分析AgWO_(3)-3的光电性质,光致发光图谱揭示Ag WO_(3)-3电子和空穴的复合得到了抑制,光电流显示AgWO_(3)-3可产生更多的光生载流子,证实了AgWO_(3)-3电子-空穴分离效率得到提升.这是Ag WO_(3)-3灭活***性能优越的原因.ROS淬灭实验表明发挥主要作用的活性氧物种(ROS)显示为·O_(2)^(-)和·OH,EPR测试进一步表明了·O_(2)^(-)和·OH的产生.基于上述结果,Ag掺杂可以作为提升WO_(3)光催化性能的有效方法,Ag-WO_(3)可以作为长期稳定的大肠杆菌光灭活催化剂.