摘要：Camelid single-domain antibody fragments(nanobodies)are an emerging force in therapeutic biopharmaceuticals and clinical diagnostic reagents in recent *** all nanobodies available to date have been obtained by animal immunization,a bottleneck restricting the large-scale application of *** this study,we developed three kinds of gene designatedregion pan-editing(GDP)technologies to introduce multiple mutations in complementarity-determining regions(CDRs)of nanobodies in *** the integration of G-quadruplex fragments in CDRs,which induces the spontaneous multiple mutations in CDRs;however,these mutant sequences are highly similar,resulting in a lack of sequences diversity in the *** also used CDR-targeting traditional gRNA-guided base-editors,which effectively diversify the *** most importantly,we developed the self-assembling gRNAs,which are generated by reprogrammed tracrRNA hijacking of endogenous mRNAs as *** base-editors guided by self-assembling gRNAs,we can realize the iteratively diversify the *** we believe the last GDP technology is highly promising in immunization-free nanobody library construction,and the full development of this novel nanobody discovery platform can realize the synthetic evolution of nanobodies in vitro.

摘要：预制装配式建筑是建筑行业的发展方向,基于此,本文谨就预制装配式建筑结构体系及其设计进行研究与探讨,以期为预制装配式建筑施工效率及工程质量的提升提供参考。

摘要：对光管冷却器和翅片管冷却器的冷却效率进行了对比实验研究 ,结合 Star- CD软件数值模拟的结果 ,指出了原翅片管冷却器的不合理结构参数设计 ,提出了翅片管冷却器结构的改进设计方案 ,实验结果表明 ,改进后的翅片管冷却器的冷却效率明显提高。

摘要：Creation of freeways in a membrane accelerates water transport for efficient desalination;however,the synthesis of target membrane materials is very *** the present study,the molecular-design strategy is developed for the synthesis of porous aromatic framework(PAF)membranes with rigid and open networks to regulate water ***-based monomers in triangle shape are designed for the construction of PAF-98 and its sister frameworks,and these PAF membranes are formed through trifluoromethanesulfonic acid *** rigidity and geometry of the monomers endow PAFs with unique structures of connected pores with edging size(4.5–6.7Å)between water(2.8Å)and hydrated sodium molecules(7.2Å),enabling effective differentiation of water and *** fabricated membranes with high flexibility and continuity are ideal for water desalination,and reverse osmosis experiments demonstrate that the three PAF membranes are selective for permeation of water molecules over hydrated sodium *** water flux of 547.1 L m^(-2) h^(-1) and high rejection degree of 90%are obtained on a representative membrane of PAF-98-E;and the desalination performance is superior to currently available membrane ***,PAF-98-E membrane is capable of rejecting various cations with high efficiency in real seawater,holding great promise in practical water desalination application.

摘要：基于高重频脉冲功率开关技术,对大功率晶闸管的快速在线检测电路进行了设计。通过分析对比晶闸管的传统检测方法及已有在线检测方法,提出了基于真空开关高压隔离技术的晶闸管快速故障在线检测设计方案。通过对晶闸管并联的均压电阻两端的电压进行静态检测,实现串联晶闸管故障的快速判别;给出了真空开关的选型结果;并通过仿真与试验验证了检测电路的可行性。该检测方法合理可行,可在0.6s内完成串联晶闸管的故障检测,对高重频脉冲电源中的关键器件晶闸管的健康管理系统有重要的工程应用价值。

摘要：目的探讨社区老年人居家护理服务需求情况及其影响因素。方法采用一般资料调查表、日常生活活动能力量表(ADL)、自行设计的社区老年人居家护理服务需求问卷对咸宁市社区523名老年人进行调查分析。结果血压监测、血糖监测、疾病知识健康教育、生活方式健康指导、服药健康指导及心理支持分别占老年人居家护理服务需求的前6位,Logistic回归分析显示,影响老年人居家护理服务需求的因素主要有年龄、平均月收入、文化程度、患病种数及日常生活活动能力。结论社区老年人对居家护理服务需求较大,高龄、患慢性病数目多、ADL能力低、文化程度及收入高的社区老年人居家护理服务需求更高。

摘要：目的 分析创客教育2.0产生的背景、内涵,以及将其引入到护理教育中的创新性;提出基于创客教育2.0的护理学混合式教学.方法 采用文献研究法、描述性研究法对创客教育2.0的应用现状及与护理教育的联系进行分析,根据分析结果采用探索性研究法设计基于创客教育2.0的护理学混合式教学框架.结果 虽然创客教育2.0已逐渐成为培养学生创新能力的重要途径,各级各类学校在尝试实施创客教育2.0,然而在护理教育领域中,目前尚未出现相关研究报道.结论探索基于创客教育2.0的护理学混合式教学可在一定程度上促进护理教育的改革;为了更加深入地探讨创客教育2.0在护理教育中的应用效果,还需针对其发展机制、支持理论等方面展开研究.

摘要：目的 从行为运作的视角,探讨个体认知如何影响医务人员血源性职业暴露防护行为.方法 以健康信念模式作为理论基础设计调查问卷,采用多级抽样法,对遵义市14所医院医务人员进行调查,应用结构方程模型分析数据.结果 样本数据与假设模型的契合度较为理想,医务人员血源性职业暴露防护行为可以被易感性认知、严重性认知、行为益处认知、行为障碍认知解释的变异量为87％.影响医务人员防护行为的因素从高到低分别为行为益处认知、严重性认知、行为障碍认知及易感性认知,其路径系数分别为0.39、0.27、-0.21、0.03.结论 易感性认知、严重性认知及行为益处认知对防护行为起正向作用,其中,行为益处认知对医务人员血源性职业暴露防护行为影响较大,行为障碍认知对防护行为起负向作用,应用健康信念模式能较好的解释医务人员血源性职业暴露防护行为.