摘要：Microorganisms have been the main sources for the production of *** of chemicals requires the development of low-cost and higher-yield *** this goal,microbial strains with higher levels of production should be first *** engineering has been used extensively over the past two to three decades to increase production of these *** in omics technology and computational simulation are allowing us to perform metabolic engineering at the systems *** combining the results of omics analyses and computational simulation,systems biology allows us to understand cellular physiology and characteristics,which can subsequently be used for designing ***,we review the current status of metabolic engineering based on systems biology for chemical production and discuss future prospects.

摘要：肿瘤已成为严重威胁人类健康的重大疾病之一。以顺铂为首的铂类抗肿瘤药物一直是化疗首选药物。但是长期用药导致的一系列的毒副作用如肾毒性、耳毒性和耐药性等极大地限制了铂类配合物的发展与应用。本文针对目前铂类药物所处形势重点综述了新一代铂类药物的设计研发方法:(1)研发具有新颖结构的铂类药物,例如经过改造的反式铂类配合物、多核铂类配合物、Pt(Ⅳ)配合物等;(2)发展新的抗肿瘤靶点,例如以G-四螺旋DNA(G4-DNA)为靶点,为寻找更有效的铂类抗肿瘤药物提供新的思路。同时通过列举最新研究成果,分析药物的抗肿瘤机理及在克服顺铂耐药性机理方面的研究进展,提出铂类药物的设计研发方法,让读者了解铂类抗肿瘤药物的发展历程和未来的发展趋势。

摘要：Escherichia coli BW25113 was metabolically engineered for CoQ10 production by replacing ispB with ddsA from Gluconobacter *** of precursor balance and reduced nicotinamide-adenine dinucleotide phosphate （NADPH） availability on CoQ10 production in *** were *** knockout of pykFA along with pck overexpression could maintain a balance between glyceraldehyde 3-phosphate and pyruvate,increasing CoQ10 *** of native NAD-dependent gapA with NADP-dependent gapC from Clostridium acetobutylicum,together with the overexpression of gapC,could increase NADPH availability and then enhanced CoQ10 *** effects,overexpressions of various genes in CoQ biosynthesis and central metabolism,different vectors and culture conditions on CoQ10 production in ***,were all *** investigation of different vectors indicated that low copy number vector may be more beneficial for CoQ10 production in *** recombinant *** （△ispB：：ddsA,△pykFA and △gapA：：gapC）,harboring the two plasmids encoding pck,dxs,idi and ubiCA genes under the control of PT5 on pQE30,ispA,ddsA from Gluconobacter suboxydans and gapC from Clostridium acetobutylicum under the control of PBAD on pBAD33,could produce CoQ10 up to 3.24 mg·g-1 dry cell mass simply by changing medium from M9YG to SOB with phosphate salt and initial culture pH from 7.0 to *** yield is unprecedented and 1.33 times of the highest production so far in ***.

摘要：介绍一个从合成、表征到性能研究的完整研究型综合实验。合成路线巧妙,制备出了文献报道的尺寸最小的纳米金属有机框架。引入纳米材料研究基本表征手段和吸附动力学研究,通过实验教学展示材料研究方法,拓展学生知识面。通过对实验条件的设计,激发学生探索精神、培养学生创新意识、训练学生化学思维。