摘要：For the surgical treatment of cardiovascular disease(CVd),there is a clear and unmet need in developing small-diameter(diameter<6 mm)vascular *** our previous work,sulfated silk fibroin(SF)was successfully fabricated as a potential candidate for preparing vascular grafts due to the great cytocompatibility and ***,vascular graft with single layer is difficult to adapt to the complex internal *** this work,polycaprolactone(PCL)and sulfated SF were used to fabricate bilayer vascular graft(BLVG)to mimic the structure of natural blood *** enhance the biological activity of BLVG,nicorandil(NIC),an FdA-approved drug with multi-bioactivity,was loaded in the BLVG to fabricate NIC-loaded *** morphology,chemical composition and mechanical properties of NIC-loaded BLVG were *** results showed that the bilayer structure of NIC-loaded BLVG endowed the graft with a biphasic drug release *** in vitro studies indicated that NIC-loaded BLVG could significantly increase the proliferation,migration and antioxidation capability of endothelial cells(ECs).Moreover,we found that the potential biological mechanism was the activation of PI3K/AKT/eNOS signaling ***,the results effectively demonstrated that NIC-loaded BLVG had a promising in vitro performance as a functional small-diameter vascular graft.