摘要：设计并实现了一种新颖的超高频RFID标签的基带处理器。该标签以ISO/IEC 18000-6C协议为基础,但在反向链路通信方面,在原协议FM0编码/Miller调制副载波的基础上增加了扩频编码的实现,目的是提高反向链路的通信信噪比。该设计支持协议要求的所有11条强制命令的读写操作,概率/分槽防冲突算法,以及对存储器的读写操作。设计中采用了低功耗技术,显著降低了芯片的平均功耗和峰值功耗。芯片采用0.18μm 6层金属CMOS工艺进行流片,面积为0.5 mm2。测试结果表明,芯片消耗功耗约为16μW,最低工作电压为1.04 V。

摘要：VPAC1是垂体腺苷酸环化酶激活多肽(pituitary adenylate cyclase-activating polypeptide,PACAP)和血管活性肠肽(vasoactive intestinal polypeptide,VIP)的共同受体。VPAC1介导PACAP和VIP抑制食欲和抗炎的生物学功能。本研究体外实验表明,VPAC1在分化成熟的3T3-L1脂肪细胞中表达增高,并且VPAC1激动剂(10～1000nmol/L每1×106cells)可诱导脂肪细胞脂解,因此我们预计VPAC1激动剂具有抗肥胖及肥胖综合征的作用。为研究VAPC1激动剂[Lys15,Arg16,Leu27]-VIP(1-7)GRF(8-27)对营养性肥胖及肥胖综合征的干预作用,本研究又设计了两组体内实验:(1)高脂喂养NIH雄性小鼠4周,同时腹腔注射VPAC1激动剂;以注射生理盐水作为对照;(2)高脂喂养NIH雄性小鼠5周,构建肥胖模型后,再腹腔注射VPAC1激动剂4周,同样以注射生理盐水作为对照。采集摄食、体重、体脂、血糖及血脂等指标。结果显示,VPAC1激动剂显著抑制摄食、抑制高脂饮食诱导的体重及体脂(附睾及背部)重量的增长,并有效改善高脂饮食诱导的高血糖及高血脂,提高机体的糖耐受。高剂量(每天50nmol/kg体重)VPAC1激动剂比低剂量(每天5nmol/kg体重)有更显著的抗肥胖作用,提示VPAC1激动剂的抗肥胖作用具有剂量依赖性。以上结果表明,VPAC1激动剂不仅能抑制高脂诱导的肥胖的发展,而且可有效改善肥胖相关疾病;其抗肥胖作用的机制是复杂整合的,值得进一步深入研究。

摘要：[Objective] The aim was to study the expression of cold resistant gene CAS19 of Gongnong No.2 Medicago sativa L. in tobacco. [Method] A pair of primers was designed according to nucleotide sequences of cold resistant gene CAS19 of M. sativa,and then RT-PCR was used to amplify the protein gene of CAS19,which was then cloned into pMD18-T vector and subcloned into expression vector PBI121. The recombination expression plasmid PBCAS was constructed. And then it was transferred into tobacco genome via Agrobacterium,and Southern-blotting analysis was used for detecting transgenic plants. [Result] CAS19 gene was integrated into the tobacco genome and highly expressed. [Conclusion] This study had provided theoretical basis for further exploring the expression mechanism of cold resistant gene CAS19 in tobacco.

摘要：精确评估模块连接处荷载特性对超大型浮体的水动力设计及结构安全性研究具有重要的工程意义。文章以5模块半潜式超大型浮体为研究对象,考虑碰撞荷载和风、浪、流环境荷载的联合作用并采用AQWA软件对超大型浮体模块连接点处的受力特性开展了数值计算。船舶碰撞荷载简化为矩形脉冲,采用定常风、定常流以及JONSWAP波谱,探讨了船舶碰撞角度、撞击位置、环境荷载等因素对模块连接点受力特性的影响。该文的研究成果可为事故荷载作用时超大型浮体连接器结构设计和强度计算提供一定依据。

摘要：为建立快速、准确的赤潮藻鉴定和定量检测方法,以红色裸甲藻(Gymnodinium sanguineum)作为研究对象,将快速检测手段--实时荧光定量PCR(RFQ-PCR)技术应用于赤潮藻的种类鉴定中.首先以红色裸甲藻18S rDNA序列为靶区域,设计出适合用于RFQ-PCR技术的红色裸甲藻引物和TaqMan探针,并通过引物PCR验证确定其种特异性;进而应用实时荧光定量PCR方法建立红色裸甲藻快速检测方法.与显微镜计数方法比较,两者所获结果无显著性差异,且该方法的检测限远低于红色裸甲藻爆发赤潮时的藻密度,因此该方法可有效地应用于红色裸甲藻的检测,从而为红色裸甲藻赤潮的预警预测奠定基础.

摘要：Dynamic simulation system of maize growth is developed by the physiological and ecological model,morphological structure model,computer science and virtual reality technology,to improve the level of precise operation of maize *** computer graphics algorithms,virtual reality technology,animation design and information integration technology are applied to maize production by this *** of dynamic simulation system of maize growth is conducive to raise level of precise operation in maize *** system also can assist the relevant production research and testing,to reduce cost and improve efficiency.

摘要：[Objective] To clone the actin gene of Cryptosporidium andersoni, and to study its eukaryotic expression in Hela cells. [Methed] Specific primers were designed for the partial encoding sequence of actin, which were obtained by screening the T7 phage display library of Cryptosporidium andersoni, and the actin gene CA42 was amplified by PCR. Recombinant eukaryotic expression plasmid pVAX1-CA42 was constructed and transfected to Hela cells with lipofection strategy. Indirect im- munofluorescence staining, SDS-PAGE and Western blotting analysis were used to detect the expression of recombinant protein in Hela cells. [Result] CA42 protein was successfully expressed in Hela cells, and the expression products had reactogenicity. [Conclusion] The partial encoding sequence of actin from Cryptosporidium andersoni has been successfully cloned, and it can be stably expressed in Hela Cells

摘要：Polyurethane elastomers with covalent adaptable networks(PU-CANs)based on the dynamic urethane bond have attracted remarkable attention owing to their reprocessability,adaptability,and ***,it is still a formidable challenge to achieve excellent dynamics at low temperatures since the urethane bond energy is usually ***,a fluorinated phenolic polyurethane(FPPU)elastomer with CANs based on phenol±carbamate bonds was successfully designed and ***,the effects of fluorine atoms on the mechanical properties,thermal stability,reprocessability,and self-healability,surface free energy,and hydrophobicity of the prepared elastomers were systematically *** FPPU elastomer showed notch-insensitivity,remarkable self-healable efficiency(98%),low dynamic dissociation temperature(60℃),excellent reprocessability,and low surface energy(62 MJ m^(-2))compared with non-fluorinated counterpart phenolic polyurethane elastomer(APPU).Based on the above-mentioned features,FPPU was selected as an elastic substrate to assemble into a triboelectric nanogenerator(TENG)to harvest energy from natural *** TENG exhibited an excellent electrical output performance with a peak open-circuit voltage of 103 V,a peak short-circuit current of 4.7μA and a peak short-circuit charge of 43 *** addition,the TENG possessed high selfcleaning and reprocessing ***,a stretchable and self-healing composite conductor based on FPPU was fabricated for flexible electronic devices.

摘要：提出一种基于线性同余生成器(LCG)来构造IDMA系统中随机交织器的方法.该方法通过选择LCG的参数使其周期达到最大.为了设计方便,取LCG的模为2的整数幂.利用文中的方法产生母交织器,再对其进行循环移位,即得到每个用户的交织器.其中超出交织范围的随机数将被丢弃.数学推导证明,在上述条件下LCG的模必须严格大于交织器的深度.利用基向量相关峰值方法和误比特率性能证明了文中方法的有效性.

摘要：[Objective] Ogura cytoplasmic male sterility （Ogu CMS） is an ideal pollina- tion control system for heterosis utilization in Brassica napus. However, fertility-restor- ing （Rf） gene only exists in radish chromosome and is closely linked with glucosino- late gene, making it hardly to be applied directly in production. Thus, the key to apply Ogu CMS in Brassica napus is to introduce the Rf genes and to break its linkage with glucosinolate gene. [Me^od] To overcome the interspecific reproductive barrier, grafting was conducted by using Raphanobrassica （2n=-58） as donor materi- als of Rf genes. The obtained interspecific hybrids were analyzed from the agronom- ic traits, seed-setting rate and fertility restoration rate for screening fertility-restoring materials. [Results] By elaborative selection, a homozygous Ogu CMS fertility-restor- ing B. napus material named CLR650 was selected out, whose somatic chromosome numbers were found to be 2n=38-40. Some abnormal phenomena like anaphase bridges and lagging chromosomes in meiosis were observed, but the abnormalities did not affect the formation of normal pollens. It can restore the male fertility in both progenies of self-pollination and testcross by 100%. Molecular analysis showed that CLR650 harbors Ogu CMS fertility-restoring gene, which is obviously different from that of Ogu CMS restore line Rl13 and RHH1 by detecting the molecular markers closely linked with radish restorer gene （Rf0）. [Conclusion] The CLR650 could be a new restorer for the Ogu CMS in B. napus.