摘要：叶色性状,作为一个形态学标记,在作物的遗传改良中扮演着重要的角色。在芥菜型油菜品系1280中发现了一个叶色自发突变体,将其命名为1280-1。1280-1新叶通常呈绿色,叶缘和叶脉紫色。随着叶片生长,整个叶片均呈现紫色。1280-1目前已自交7代,叶片紫色性状已稳定。为进一步研究1280-1的遗传特性,将1280-1与2个芥菜型油菜绿叶亲本(芥菜型多室油菜和富源油菜)进行杂交,F1自交后获得F2群体,同时F1与芥菜型油菜绿叶亲本进行回交得到BC1分离群体。F2分离群体统计表明,1280-1的紫叶性状受到1对显性基因控制,将该基因命名为Bjpl1;利用AFLP结合BSA的方法,设计512对引物组合,在1280-1与芥菜型多室油菜构建的BC1分离群体筛选与Bjpl1基因紧密连锁的分子标记,共获得10对与目标基因紧密连锁的分子标记。其中,PL02、PL07和PL10与Bjpl1基因共分离;PL07被转化成一个SCAR标记。目标基因两侧最近的标记分别为PL01(PL04)和PL08,它们与目标基因间的遗传图距分别为0.7和1.3cM。

摘要：本文对海洋来源的内生真菌棘孢曲霉(Aspergillus aculeatus)的次级代谢产物进行了研究,从其提取物中分离得到一个新的具有较长交叉共轭系统的甾体类化合物,通过各种波谱分析方法确定其结构为15α-羟基-(22E,24R)-麦角甾-3,5,8(14),22-四烯-7-酮(15α-hydroxy-(22E,24R)-ergosta-3,5,8(14),22-tetraen-7-one)。测试了该化合物在体外对小鼠白血病细胞株P388、人早幼粒白血病细胞株HL-60、人前列腺癌细胞株PC-3的细胞毒作用。

摘要：[Objective] This study aimed to reconstruct the HP intein by overlap-exten- sion PCR. [Method] Several primers were designed according to the principle of overlap-extension PCR that the repeat sequences were overlapped and served as the template to the other DNA strand in amplification. Then, several rounds of over- lap PCR reaction were conducted to mutate the four cysteines in the HP intein into serines, and introduce a linker sequence containing a tag for product detection and purification. [Result] DNA sequencing analysis proved that the four cysteines in the HP intein were successfully mutated into serines; the PCR precursor fragments were also rejoined into an intact HP gene fragment, without introducing any other unex- pected mutation; the DNA linker of 46 bp was successfully inserted into the de- signed HP gene sequences, without any mutation and base pair mismatch. [Conclu- sion] The HP intein was rapidly reconstructed by overlap-extension PCR in this study, which not only expands the application of overlap PCR in protein engineering, but also provides a simple, efficient and highly accurate tool for the reconstruction and modification of intein.

摘要：将逆系统方法与模糊神经网络相结合,提出一种基于模糊神经网络α阶逆系统的发酵过程解耦控制方法.在分析了系统可逆性的基础上,利用模糊神经网络建立发酵过程的非线性逆模型,然后将得到的模糊神经α阶逆系统与发酵过程串联复合成伪线性系统,最后设计专家控制器实现高性能闭环解耦控制.仿真结果表明,提出的解耦控制方法能够适应发酵过程模型的不确定性和参数的时变性,具有较强的鲁棒性,克服了解析逆系统解耦控制方法依赖于过程模型和对模型参数的变化很敏感的缺点,且结构简单,易于实现.

摘要：目的:制荡挡板由于其安装方便并可以大幅减弱液舱半载时的晃荡效应而备受关注。本文基于试验验证的计算流体动力学方法,对环型挡板的制荡效应影响因素进行分析,以确定环形挡板中影响制荡效率的主要参数。创新点:1.通过正交设计与数值模拟的结合,得到影响环形挡板制荡效率的主要因素。2.结合正交试验的分析结果及环形挡板制荡机理数值分析,得出改进环形挡板制荡效率的方案。方法:1.通过物理试验对数值模拟方法进行验证;2.通过正交试验设计,制定环形挡板参数的数值试验表(表7),并根据计算结果,对参数的制荡效率影响进行分析(表8和9);3.对主影响参数进行重点分析,得出其制荡效率的变化规律(图19);4.对环形挡板的制荡机理进行分析,并对正交试验结果中各参数水平的影响进行验证。结论:1.环形挡板的高度、倾斜角度以及安装位置均对其制荡效率有显著影响,而环形挡板的厚度影响较小。2.环形挡板的4个参数中,高度因素的影响效果最显著,但是,当高度增至罐体直径的20%时,制荡效率提升速度变得不明显。3.相比于倾斜挡板会产生较多涡流耗散,当环形挡板处于竖直状态时,其较强的阻隔效应可以提供更多的制荡效果;挡板倾斜的方向对制荡效果有一定的影响,但该影响会随着倾斜角度的增大而减小。4.增大两个环形挡板的间距,将增加自由液面的长度,并延缓挡板约束流速的效率,从而减弱挡板的制荡效果。

摘要：K+通道亚型Kv4.3在调节心肌细胞动作电位的幅度与时程方面具有重要作用,是治疗心律失常的有效作用靶点,但目前世界上该通道的特异性抑制剂非常缺乏。敬钊毒素-V(Jingzhaotoxin-V,JZTX-V)是从敬钊缨毛蜘蛛粗毒中纯化到的一种新型肽类神经毒素,能够部分抑制大鼠背根神经节细胞上的瞬时外向K+电流,其半数有效抑制浓度(IC50值)为52.3nmol/L。为了研究JZTX-V对Kv4.3通道的作用,本实验通过多肽固相化学合成的方法得到JZTX-V,并用双电极杆电压钳技术检测JZTX-V对表达在非洲爪蟾卵母细胞上的Kv4.3通道电流的作用。结果显示,JZTX-V能够完全抑制Kv4.3通道电流,并且这种抑制作用具有浓度依赖性和时间依赖性,其IC50值为425.1nmol/L,JZTX-V还能够使通道的电流-电压关系曲线和稳态失活曲线分别向去极化方向漂移大约29mV和10mV,改变Kv4.3通道的动力学特征,因此我们推测JZTX-V是一种Kv4.3通道门控调制毒素。以上研究结果对于开发心肌Kv4.3通道的分子探针及以Kv4.3通道为靶点的药物设计具有借鉴作用。

摘要：***-based agricultural machinery monitoring WEBGIS is flexible and dynamic,but this flexibility and dynamic characteristics reduce the performance of ***,it is necessary to use built-in optimization features *** Framework,some performance optimization techniques in program design and *** cache technology to reduce the loading of server,and make the designed system work more efficiently.

摘要：[Objective] The aim was to study the cloning and sequence analysis of rbcS gene of wild barley under salt stress. [Method] The tender leaf blade of wild barley under salt stress was taken as the experimental material. The primers were designed according to the homology of rbcS gene sequences of wheat and barely in Genbank; then PCR amplification,recovery,ligation,transformation and sequencing of rbcS gene were carried out. [Result] Two rbcS genes including rbcS1 and rbcS2 with the length of 1 252 and 908 bp respectively were cloned from the barely genome. rbcS1 and rbcS2 were both composed by two exons and one intron. The exons length of the two genes was the same of 525 bp,encoding 174 amino acids,and the homology between them was 96%; however,the intron length of rbcS1 and rbcS2 was 448 and 107 bp respectively.

摘要：针对微电网孤岛运行时有功和无功分量严重耦合引起的无功功率无法精确分配和增加虚拟电抗带来的电压跌落问题,在虚拟同步发电机(virtual synchronous generator,VSG)一次电压控制器的基础上,提出一种在线计算电压控制器参考输入电压的新算法,根据调度有功和无功功率预先估算感应电动势,补偿VSG固有下垂特性和线路阻抗的电压跌落,保证VSG按照设定的U-Q下垂特性运行;设计二次电压控制,用以解决上层调度的周期性、负荷波动的随机性及一次电压控制的有差性等带来的电压质量问题;最后,在Matlab/Simulink仿真平台上验证了所提方案的效果。

摘要：A population of 140 recombinant inbred lines at F8 generation were obtained after seven successive generations of self-pollination using single seed descent（SSD） method from the F2 hybrids of three-line restorers Luhui 8258 with high combining ability and Yanghui 34. Then, the 140 inbred lines obtained above and their parents Luhui 8258 and Yanghui 34 were crossed with three different types of cyto-plasmic male sterile（CMS） lines（Gang 46 A, Ⅱ-32 A and Lu 98A） according to NCⅡ design. The resulting 426 combinations were planted at Deyang and Suining bases to test the combining ability and inheritance of five yield traits： yield per plant, panicle number per plant, filled grain number per panicle, seed setting rate and 1 000-grain weight. The results showed that the variances of both general and specific combining abilities of the five traits all reached a significant or extremely significant level at the two sites. The broad and narrow heritability of the yield traits（except 1 000-grain weight whose broad and narrow heritability were both over70%） were all below 50% at the two experimental bases, suggesting that the four traits were easily subjected to environment influence. Very significant positive correlation of general combining ability was found between yield per plant and other traits except 1 000-grain weight. The general combining ability variance showed a normal distribution among the recombinant inbred lines at two sites, right in line with inheritance of quantitative traits. So, the genes controlling rice general combining ability can be targeted by QTL mapping.