摘要：Laminar optical tomography(LOT)is a mesoscopic tomographic imaging technique ranging between confocal microscopy and diffuse optical tomography(DOT).Fluorescence LOT(FLOT)provides depth-resolved molecular information with 100-200μm resolution over 2-3mm *** this study,we use Monte Carlo simulation and singular-value analysis(SVA)to optimize the source-detector configurations for potential enhancement of FLOT imaging *** effects of different design parameters,including source incidence and detector collection angles,detector number,and sampling density,are *** results indicate that angled incidence/detection configuration might improve the imaging resolution and depth sensitivity,especially for low-scattering *** the number of detectors and the number of scanning steps will also result in enhanced imaging *** also demonstrate that the optimal imaging performance depends upon the background scattering coe±*** result might provide an optimization strategy for FLOT or LOT experimental setup.