摘要：This paper proposes a new cell intuition simulation method which is a combination of intuitive simulation calculation method and the operation of binary image, and applied it in the innovation of the graphic design process. First of all, we study how to express a variety of graphics, and establish the definition of cell intuitive model, work out the cell intuitive operation process and many new cellular operators such as a variety of matrix blocks crossover operator, a variety of matrix blocks mutation operator, matrix blocks replace operator, matrix blocks compression operator, matrix blocks extension operator. By choosing two or more cells and selecting the artificial selection or fitness selection, we can set up and visualize the design and pick the best design results .Finally, validation is made on this algorithm by an example, and a innovation graphic is also represented.

摘要：Migrating legacy system with web service is an effective and economic way of reusing legacy software in a SOA *** this paper,we present an approach for migrating a three-tie object-oriented legacy system to SOA *** key issue of the approach is about services identification from large numbers of *** we propose a bottom-up method to model the system with UML and identify services from UML *** approach can be a reference to an auto-migrating process.

摘要：β-1,3-葡聚糖酶是一种植物病程相关蛋白,在植物抵御病害中有重要作用。本研究以毛竹为实验材料,利用RACE技术,克隆毛竹β-1,3-葡聚糖酶基因的外显子序列,并在β-1,3-葡聚糖酶基因的编码区两端设计引物,以毛竹基因组DNA为模版扩增该基因的内含子序列。序列结果表明,该基因全长1693bp,包含两个外显子和一个内含子,命名为PheGLU(GenBank登录号GU238236)。该基因包含1个996bp的开放阅读框,编码332个氨基酸,相对分子质量为3.541×104Da,其等电点pI为6.389,是一个酸性蛋白且分泌到胞外;其中,该蛋白的二级结构中包含35.15%的α-螺旋,21.82%的β-转角,43.03%的延伸链和无规则卷曲等;三级结构同源建模预测显示,它与大麦β-1,3-葡聚糖酶(PDB number:1ghsA)具有80.4%的同源性;聚类分析显示,该基因序列与已报道的其它植物具有较的高氨基酸序列同源性。本研究为进一步鉴定毛竹β-1,3-葡聚糖酶基因的抗真菌病害能力奠定了基础。