摘要：To implement high quality tunneling injection quantum dot lasers, effects of primary factors on performance of the tunneling injection quantum dot lasers were investigated. The considered factors were tunneling probability, tun- neling time and carriers thermal escape time from the quantum well. The calculation results show that with increas- ing of the ground-state energy level in quantum well, the tunneling probability increases and the tunneling time decreases, while the thermal escape time decreases because the ground-state energy level is shallower. Longitudinal optical phonon-assisted tunneling can be an effective method to solve the problem that both the tunneling time and the thermal escape time decrease simultaneously with the ground-state energy level increasing in quantum well.

摘要：无细胞表达蛋白质体系近年来恢复活力,以满足日益增长的蛋白合成需求.与传统的细胞重组表达蛋白的方法相比,无细胞体系具有许多优点,包括更快的速度、更高的通量和更大的灵活性.此外,它提供了独特的可能性,如合理的DNA模板设计,毒素蛋白的表达,多个多肽链的联合表达,高效的定位标记等等.在本文中,建立了一个linker-tRNA的方法利用无细胞体系固相合成蛋白质.首先,需要将第1个氨基酸连接上起始tRNA通过linker连接到固相表面.为了提高固相合成蛋白质的效率,利用含有琥珀终止子(CUA)为反密码子的linker-tRNA与含互补琥珀子(UAG)为起始密码子的DNA模板而不是正常的起始密码子(密码子AUG).已经成功地获得了纯化的fma-tRNA(-CA)和linker-Met了.此方法将为蛋白质芯片的开发提供新途径.