摘要：[Objective] This study aimed to investigate the functions of transgenic growth related gene in pig growth. [Method] A pair of primers containing Nhe I and Hind Ⅲ restriction sites were designed by referring to the pig Ghrelin mRNA sequence published in Genbank. Total RNA was extracted from the small intestine tissue of 13/17 Robertson translocation heterozygous pig, and then was purified and used as the template in later RT-PCR reaction to amplify the full-length pig Ghrelin gene. The correct pig Ghrelin gene fragment was cloned into the pMD19-T simple vector for sequencing analysis. The obtained full-length cDNA of pig Ghrelin gene fragment was digested with both Nhe I and Hind Ⅲ, and then was linked into the eukaryotic expression vector pEGFP-N1 to obtain the recombinant plasmid pEGFPGhrelin. The recombinant plasmid was transected into the fibroblast cells to detect the fluorescence labeled gene expression. [Result] The nucleotide sequence extracted from 13/17 Robertson translocation heterozygous pig was the same as expected; and the eukaryotic expression vector pEGFP-Ghrelin was successfully constructed. [Conclusion] The eukaryotic expression vector constructed in this study can be further used in research on transgenic pigs, but also lays foundation for research on the regulatory mechanism of Ghrelin gene.

摘要：电流互感器(current transformer,CT)的暂态饱和容易导致继电保护误动作,寻求暂态饱和的原因和防范保护误动的对策特别需要建立CT的分析模型,然而该模型及其参数的构建如何体现实际运行一直是个研究难点。该文首先基于J-A理论给出了CT分析模型,分析了该模型的数值计算思路。然后针对J-A模型中描述不同励磁曲线轨迹的关键参数,研究了以磁感应强度B为优化目标的引力搜索辨识方法。最后,精心设计了330k V系统P级CT的6~48k A大通流动态模拟试验,测试表明CT的J-A模型以及所提的参数辨识算法是有效的、正确的并且符合工程实际。