摘要：确定C型产气荚膜梭菌合成培养基使用参数及建立配套的毒素浓缩工艺。比较不同pH值、灭菌温度、配制用水的合成培养基及其不同培养时间和温度下培养产气荚膜梭菌CVCC60102株的毒力；按毒素的分子量及超滤膜的截留分子量设计并比较2种不同浓缩工艺的毒素收获率及透出液的毒力。

摘要：确定C型产气荚膜梭菌合成培养基使用参数及建立配套的毒素浓缩工艺。比较不同pH值、灭菌温度、配制用水的合成培养基及其不同培养时间和温度下培养产气荚膜梭菌CVCC60102株的毒力;按毒素的分子量及超滤膜的截留分子量设计并比较2种不同浓缩工艺的毒素收获率及透出液的毒力。

摘要：该文在已有的乳酸发酵全合成培养基基础上,研究了氨基酸和维生素类组分对菌体生长和乳酸生成的影响。通过单组分浓度梯度实验确定了对菌体生长和乳酸合成有明显影响的6种氨基酸和3种维生素;以此作为Plackett-Burman实验设计的因素,筛选出了生物素、天冬氨酸、谷氨酸3种对于乳酸生物合成有极显著影响的因素;通过最陡爬坡实验确定3种因素具有最大响应值的浓度范围;并最终通过Box-Behnken实验得出3种因素的最佳添加浓度。在250 mL摇瓶和2.5 L发酵罐中对优化后的培养基进行验证实验,发现优化后合成培养基的乳酸产量、产率和糖酸转化率都较优化前有了显著提高。该实验研究结果为该菌株今后的代谢调控研究奠定了基础。

摘要：A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plackett-Burman(PB) design and response surface methodology(RSM).The effects of 19 medium components were investigated by PB *** of these components were determined to have significant effects on ethanol *** statistical model was constructed via central composite design(CCD) using five selected variables,including xylose,trisodium citrate,choline chloride,pyridoxine,and *** validity of the developed model was *** ethanol concentration in the optimized medium was 6.7% higher than in the RM *** optimized medium was then simplified to give the final synthetic medium(S2),in which the ethanol concentration was 20.7% higher than in the RM *** and trisodium citrate were the key medium components influencing ethanol production,while calcium pantothenate was the only growth factor required by recombinant *** production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1-1.6 g/L.