摘要：目的探讨替勃龙对切除卵巢大鼠骨强度的影响。方法选用6月龄未交配健康雌性Sprague-Dawley大鼠共40只,随机分为4组(每组10只)①正常对照组或假手术组(Sham),②空白对照组即骨质疏松组(OVX),③OVX加戊酸雌二醇(ValerateEstriolVE)组(0.8mg/kg),④OVX加替勃龙(Tibolone)组(0.25mg/kg);行去势手术3周后开始按分组设计喂药(灌胃),共治疗3个月后处死。取大鼠右股骨及第3腰椎进行骨密度测定;之后对右股骨标本进行中段三点弯曲试验,测定股骨干强度指标;取大鼠第4、5腰椎制作不脱钙病理切片后进行图像分析计量。统计学处理P<0.05为具有统计学意义。结果①骨密度椎骨及股骨干骺端骨密度OVX组明显下降,使用戊酸雌二醇能够提升骨密度;但股骨干骨密度各组间差异无显著性。②骨强度三点弯曲实验结果显示OVX组股骨干强度较假手术组明显下降,替勃龙组骨干强度较OVX组提升30%(P<0.05)。③椎骨形态计量结果OVX组与假手术组、戊酸雌二醇组及替勃龙组间差异均有显著性。结论短期小剂量替勃龙治疗能阻止卵巢切除导致的成熟雌性大鼠股骨干强度受损。

摘要：Objective: To investigate the effects of hormone therapy, estrogen therapy and tibolone on markers of apoptosis including bcl-2, and bax and cyclin D 1 expression in postmenopausal vaginal epithelium. Study design: Thirty postmenopausal women were randomized to the treatment protocols (0.625 mg conjugated equine estrogen (CEE) ? 2.5 mg medroxyprogesterone acetate (MPA); 2 mg estradiol valerate; 2.5 mg tibolone). After baseline vaginal biopsy, control biopsies were performed after 70 days following the initiation of the therapy. Bcl-2, bax, Bcl-2/bax ratio, cyclin D1 measurements were performed immunohistochemically. Data were analyzed by Kruskal-Wallis, Mann-Whitney U and Wilcoxon tests. Results: After the treatment period the above-mentioned parameters were not different among the groups except for cyclin D1 levels. Cyclin D1 expression was found to be strong in patients with treated estradiol valerate. Conclusions: The effects of estrogen on cyclin D1 expression were not detected with tibolone or with the addition of progesterone to estrogen in the vaginal epithelium. Cyclin D1 appeared to have stronger effects on the estrogen related proliferation compared to apoptotic markers in vaginal epithelial cells.