摘要：Menisci are fundamental fibrocartilaginous organs in knee joints. The injury in meniscus can impair normal knee function and predisposes patients to osteoarthritis. This study prepared decellularized meniscus scaffolds using a 1% （w/w） sodium dodecyl sulfate solution and sufficient rinsing steps. Complete cell removal was verified by hematoxylin and eosin staining and DNA content assay. Decellularized menisci had accordant tension properties to intact ones, but with declined compression properties. This occurred because the collagen fiber was not damaged but glycosami- noglycans was significantly lost during the decellularization process, which was confirmed by biochemical assay and histology staining. In vitro cytotoxicity assay demonstrated that decellularized meniscus scaffolds have no toxicity on L929 murine fibroblasts and porcine chondrocytes. Further experiment showed that porcine chondrocytes could adhere and proliferate on the scaffold surface, and some cells even could infiltrate into the scaffold. All results showed the potential of this decellularized meniscus to be the scaffolds in tissue engineering.

摘要：In order to investigate the effect of different C4 linkage moieties on the cytotoxicity of podophyllotoxin derivatives, novel 4-N- and 4-C-substituted 4＇-O-demethylepipodophyllotoxin derivatives were designed and synthesized. All the compounds were tested against A549 and MCF-7 tumor cells in vitro, and six compounds showed significant cytotoxicity. The most active compound 9f was superior to GL-331, and exhibited potent cytotoxicity with IC50 value at 10^-7 mol/L level.

摘要：The therapeutic application of deoxypodophyllotoxin(DPT) is limited due to its poor water solubility and stability. In the present study, the micelles assembled by the amphiphilic block copolymers(m PEG-PDLLA) were constructed to improve the solubility and safety of DPT for their in vitro and in vivo application. The central composite design was utilized to develop the optimal formulation composed of 1221.41 mg m PEG-PDLLA, the weight ratio of 1 : 4(m PEG-PDLLA : DPT), 30 m L hydration volume and the hydration temperature at 40 oC. The results showed that the micelles exhibited uniformly spherical shape with the diameter of 20 nm. The drug-loading and entrapment efficiency of deoxypodophyllotoxin-polymeric micelles(DPT-PM) were about(20 ± 2.84)% and(98 ± 0.79)%, respectively, indicating that the mathematical models predicted well for the results. Compared to the free DPT, the cytotoxicity showed that blank micelles possessed great safety for Hela cells. In addition, the DPT loaded micelle formulation achieved stronger cytotoxicity at the concentration of 1 × 10^(-7) mol·L^(-1), which showed significant difference from free DPT(P < 0.05). In conclusion, the micelles were highly promising nano-carriers for the anti-tumor therapy with DPT.

摘要：The antibacterial properties of nano-metal oxides （ZnO, CuO） are based on the formation of reactive oxygen species （ROS）. This work reveals that the antibacterial properties of these nano-metal oxides are strongly dependent on their crystalline structure. The antibacterial activity of the nanooxides was tested against four types of bacteria that commonly cause nosocomial infections. The sonochemical method was applied not only for synthesis of nanooxides but also to their coating on textiles. The antibacterial properties of textiles coated with commercial and sonochemically prepared nano-metal oxides were evaluated and compared. The toxicity was evaluated on human lung cells and amphibian embryos, as representative models for inhalation and aquatic toxicology. The sonochemically prepared metal nanooxides are better antimicrobials than commercially available metal oxides with the same particle size range. It was found that the crystallites which have more defects and less organized structure are more toxic. The formation of ROS was studied by electron spin resonance （ESR） measurements for both the sonochemically prepared and commercial samples of ZnO/CuO nanoparticles. A significant increase in the production of radical species was found in the more defective, sonochemically prepared samples, as compared to the commercial ones. Since modulation of the nanoparticle defects influenced their toxicity, the possibility of engineering safer nano-antibacterials is indicated.

摘要：Engineering complex nanocomposites that specifically target the hepatitis B virus （HBV） and overcome the limitations of current therapies such as limited efficacy and serious side effects is very challenging. Here, for the first time, the antiviral effect of engineered plasmonic gold and layered double hydroxide self-assemblies （AuNPs/LDHs） is demonstrated, using HBV as a model virus and hepatoma-derived HepG2.2.215 ceils for viral replication, assembly, and secretion of infectious virions and subviral particles. AuNPs/LDHs were obtained by a simple, cost-effective procedure in which small AuNPs （-3.5 nm） were directly obtained and organized on the surface of larger LDH nanoparticles （-150 nm） by exploiting the capability of MgLDH, ZnLDH, and MgFeLDH to manifest their ＂structural memory＂ in the aqueous solution of Au（O2CCH3）3. The self-assembly approach of AuNPs and LDHs was assessed by transmission electron microscopy （TEM）, X-ray photoelectron spectroscopy （XPS）, powder X-ray diffraction （PXRD）, and UV-Vis analysis （UV-Vis）. All AuNPs/LDHs tested reduced the amount of viral and subviral particles released from treated cells by up to 80% and exhibited good cytocompatibility. AuNPs/MgFeLDH showed the highest antiviral HBV response with more than 90% inhibition of HBV secretion for the whole concentration range. Preliminary studies on the mechanism of HBV inhibition reveals that in the presence of AuNPs/LDHs, HBV particles are sequestered within the treated cells. The antiviral and low cytotoxic plasmonic properties of these Au/LDH nanocomposites indicate that they hold significant potential to be tailored as novel efficient therapeutics for the treatment of hepatitis B.

摘要：Four eburicane-type triterpenoids with malonyl modifications,namely irpexoates A-D(1-4),were isolated from the fruiting bodies of the medicinal fungus Irpex *** structures of the new compounds were established by extensive spectroscopic methods,including 1D and 2D NMR,HRESIMS spectroscopic *** B(2)displayed weak cytotoxicity against four human cancer cell lines(A-549,SMMC-7721,MCF-7,SW480)with IC50 values varying from 22.9 to 34.0 pM,and irpexoate D(4)showed weak cytotoxicity against the human cancer cell line SW480 with an IC50 value of 35.2μM.