摘要：The effect of material granularity, extraction times, extraction time, mi-crowave power and solid-liquid ratio on the yield of polysaccharide of dendrobium huoshanense in microwave-assisted extraction was discussed through single factor experiment. The key factors in polysaccharide extraction and the advantages over the traditional extraction method were studied based on orthogonal experiment. The results showed that the influences became unobvious with the promotion of the fac-tor’s level. The orthogonal experiment showed that the optimal factor in the mi-crowave-assisted extraction was that the sample was extracted for four times with an extraction time of 40 min under 550 W. Al these results would lay a founda-tion for exploring a new method for the extraction of polysaccharide from Dendrobi-um huoshanense.

摘要：Understanding the intra-population genetic diversity and population genetic structure is beneficial for plant protection and *** huoshanense is an endangered authentic medicinal *** analyze the population differentiation and genetic diversity in ***,simple sequence repeat(SSR)markers were obtained by high-throughput transcriptome sequencing,and 10 expressed sequence tag(EST)-SSRs were studied in 182 dendrobium individual samples from 15 populations distributed in Huoshan County,southwest Anhui *** deep sequencing of *** transcriptome,a total of 58202 unigenes were obtained,and 7781 SSRs with 1~6 base repeats were *** them,dinucleotide SSRs were the dominant repeating motifs(3119,40.085%),and the most common was AG/CT(2666,54.01%).Ten SSR primers were screened out from the designed 120 primer pairs for further polymorphism *** results showed that 75 alleles were detected,and the number of alleles at each locus ranged from 4 to 11,with an average of *** mean expected heterozygosity(H_(e)),observed heterozygosity(H_(o))and polymorphism information content(C_(pi))of the 10 SSR loci were 0.646,0.574,and 0.607,*** populations exhibited a high level of genetic diversity(H_(o)=0.591,H_(e)=0.557)and a moderate level of differentiation(F_(ST)=0.10069),and the genetic variation mainly existed within *** results of Structure and neighbour-joining(NJ)analysis based on 10 polymorphic primers showed that *** populations were clustered into 2 groups,and the populations KS,HSD,and JXZ had rich genetic diversity and multiple private alleles,suggesting that these populations could be used for genetic improvement of the *** study revealed frequency distribution of *** SSR loci,and successfully developed microsatellite genetic markers,which are expected to expand the repertoire of DNA markers for genetic study of ***.