摘要：Objective:To classify 21 new isolates of Trypanosoma cruai(***) according to the Discrete Typing Unit(DTU) which they belong to,as well as tune up a new pair of primers designed to detect the parasite in biological ***:Strains were isolated,DNA extracted,and classified by using three Polymerase Chain Reactions(PCR).Subsequently this DNA was used along with other isolates of various biological samples,for a new PCR using primers ***,the amplified fragments were ***:It was observed the predominance of DTU i in Colombia,as well as the specificity of our primers for detection of ***,while no band was obtained when other species were ***:This work reveals the genetic variability of 21 new isolates of *** in *** primers confirmed their specificity for detecting the presence of ***.

摘要：随着二代测序技术的快速发展,基因测序成本迅速下降,这导致基因数据的爆炸式增长,基因数据分析工具逐渐无法满足如此大规模的数据分析需求.一方面,基因数据分析工具大多仍为串行执行,无法有效地利用多核结构提升性能并导致计算资源的严重浪费;另一方面,由于前期设计和开发的局限性,分析工具所依赖的底层算法库不能兼顾高性能与友好的用户接口.gene Panel是当前主流的面向癌症检测的基因数据分析流程,它也是由多种基因数据分析工具组成的.该文面向gene Panel流程:(1)设计并实现了一套全新的并行gene Panel基因数据分析流程,通过数据并行和任务并行两种主要并行手段并结合负载均衡等其他优化方法,有效地提升了多核平台的资源利用率,并获得了4~7倍的整体加速比;(2)设计并实现了一种接口友好的高性能基因数据分析底层库HCC.由于相似的算法特征,该文的优化方法同样适用于除gene Panel外的其他测序流程.

摘要：Despite major achievements in the treatment ofchronic hepatitis C with the combination ofinterferons and the nucleoside analog ribavirin themajority of patients with chronic hepatitis C virus(HCV) infection cannot be treated *** this response rate we used antisensetechnologies to inhibit HCV translation as possibleadditional option for experimental *** oligodeoxynucleotides(ODN) are

摘要：This study was performed to demonstrate the transportation of an engineered MSC-produced intracellular anticancer gene product between mesenchymal stem cell (MSC) and cancer cells.? MSC-mediated anticancer strategy has held great promise owing to MSCs’ capacity of tumor-directed migration and the availability of specific anticancer genes.? All anticancer genes that have been used in previous MSC-mediated anticancer studies were limited in functioning via extracellular mechanisms, mainly because of the restriction by cell membrane to macromolecules including proteins.? In order to apply the majority of potent anticancer genes to the MSC-mediated anticancer system, a specifically designed expression vector which bears an intracellular anticancer gene, PTEN, is utilized to demonstrate the feasibility of the system in cancer therapies.? A transacting activator of transcription (TAT) was introduced into an expression vector followed by a segment for PTEN-RFP fusion protein.? A direct demonstration of PTEN-RFP transportation between MSC and cancer cells was obtained from direct co-cultures.? A marked cancer cell death was observed in indirect co-cultures with conditioned media from PTEN-transfected MSCs.? The demonstration of PTEN-engineered MSC-produced PTEN transportation indicates the feasibility of applying intracellular anticancer gene expression system in MSC-mediated strategies for cancer therapy.

摘要：[Objective] The study was to understand the subcellular localization of OsWRKY78 protein in plants. [Method] Primers specific for OsWRKY78 gene were designed according to the OsWRKY78 full length sequence in Genbank. The gene was cloned by RT-PCR method. The gene was then recombined into a plasmid expression vector carrying green fluorescent protein (GFP) gene, pBinGFP. The recombinant was confirmed by PCR and enzyme digestion. The recombinant plasmid pBinGFP-OsWRKY was transformed into Arabidopsis through Agrobacterium tumefaciens strain GV3101 and transgenic plants were obtained. [Result] Measured by fluorescence microscopy, the expression of OsWRKY78 and GFP fusion protein in root tip cells was localized in the nucleus. [Conclusion] This study laid the foundation for further investigating the function of OsWRKY78 gene and its role in related signal transduction and provided theoretical basis for exploring the relation between OsWRKY78 gene and brown planthoppers.

摘要：组学技术在系统水平上对细胞代谢进行全面的分析,极大地促进了代谢工程的发展和应用。全基因组水平的转录分析可以使研究者更加精确地评估细胞表型,加深对细胞代谢的理解。而且转录组分析也有助于研究者鉴定菌种改良的目标基因,加速对微生物细胞工厂的合理设计及构建。文中介绍了3种主要转录组平台技术的原理,并总结了转录组学在代谢工程领域中应用的最新进展和未来发展趋势。

摘要：Synthetic biology aims to endow living cells with new functions by incorporating functional gene networks into *** overexpressing,blocking and rewiring native gene pathways,synthetic biologists have harnessed this promising technology to reprogram cells to perform diverse tasks such as drug discovery,biopharmaceutical manufacturing,gene therapy and tissue engineering,*** this review,we focus on current technologies of synthetic biosensors for disease *** start with the design principle of synthetic *** we move towards the characteristics of simple synthetic biosensors,which can respond to a single input signal,and complex synthetic biosensors including Boolean gate biosensors,cascade biosensors,time-delay biosensors,oscillator biosensors and hysteretic biosensors,which can respond to more than two input signals and perform complex *** biosensor has showed great potential in disease detection,but it is still in its infancy *** efforts should be made in identifying and constructing clinically relevant regulation *** tools are also needed in the design process in order to guarantee the precision of the synthetic *** ultimate goal of a synthetic biosensor is to act as a therapeutic sensor-effector device that connects diagnostic input with therapeutic output and therefore provides all-in-one diagnostic and therapeutic solutions for future gene-and cell-based therapies.

摘要：目的:构建人B细胞易位基因2(BTG2)真核表达载体,并在HeLa细胞中表达携带FLAG标签的BTG2蛋白,为阐明BTG2基因的功能提供实验工具。方法:利用PCR法获得全长BTG2片段,将扩增得到的片段插入真核细胞表达载体pcDNA3.1(+)的多克隆位点;然后按照FLAG序列设计并合成寡核苷酸片段,插入pcDNA3.1(+)-BTG2载体,构建pcDNA3.1(+)-FLAG-BTG2载体;将以上重组质粒转染HeLa细胞,将细胞分为转染pcDNA3.1(+)空载体组、转染pcDNA3.1(+)-BTG2组和转染pcDNA3.1(+)-FLAG-BTG2组,采用抗FLAG抗体的Western blotting法,检测FLAG-BTG2融合蛋白在HeLa细胞中的表达水平。结果:将全长BTG2片段链接于pcDNA3.1(+)质粒,经限制性核酸内切酶BamHⅠ酶切分析及DNA测序证实载体序列准确;该载体转染HeLa细胞后用抗FLAG抗体进行Western blotting法检测,在空载体组和pcDNA3.1(+)-BTG2组HeLa细胞中检测不到FLAG融合蛋白的表达,而在转染pcDNA3.1(+)-FLAG-BTG2组中检测到FLAG-BTG2融合蛋白的表达。结论:成功构建了pcDNA3.1(+)-FLAG-BTG2真核表达载体,并能在HeLa细胞中有效表达携带FLAG标签的BTG2蛋白。

摘要：Cucumber mosaic virus is one of the most constraints to the production of tomato and other vegetable crops worldwide. Here, we generated an RNAi construct containing inverted repeat of 1138 bp fragment of a partial replicase gene of CMV-O and used it to produce transgenic tomato plants expressing CMV-specific dsRNA of the replicase gene. Inoculation of transgenic plants with CMV strain O discriminated three categories of plants: plants that showed complete resistance, which were free of symptoms;highly resistant plants, which had mild symptoms, but later recovered because new leaves that emerged were free of symptoms;and susceptible plants, which showed severe symptoms similar to wild-type plants. The completely resistant lines were selected and challenged with a closely related strain, CMV-Y. Interestingly, the transgenic plant lines either remained immune or showed high levels of resistance to the strain. No virus could be detected in uninoculated new leaves of the resistant lines after RT-PCR and Dot immunobinding assay (DIBA) analyses. We could show that the resistance is correlated with post-transcriptional gene silencing because of the production of transgenic specific siRNA.

摘要：基于高通量数据,研究人员已经设计了许多算法用于寻找功能完全未知蛋白质的功能.然而,这些算法的效率受到一些根本因素的制约,包括:(ⅰ)功能完全未知的蛋白质参与一个精细功能的先验概率低;(ⅱ)高通量互作数据中有大量的假阳性互作;(ⅲ)蛋白质互作数据对功能类的覆盖不完全;(ⅳ)训练算法的大量阴性样本数据是异质的;(ⅴ)训练算法的蛋白质的精细功能知识不足.因此,本研究提出一种新的方法对已知部分功能的蛋白质进行功能预测,即利用功能特异的蛋白质互作子网或者基因表达模式信息来寻找蛋白质更为精细的功能.该方法能够通过恰当地定义预测范围和过滤假阳性数据减少上述提到的问题,因此可以高效地发现蛋白质的新功能.对于几千个已知部分功能的酵母与人类蛋白质,该方法能够以超过90%的精确率找到它们更为精细的功能.预测的精细功能对于指导随后的湿实验和提供必要的功能知识来学习其他蛋白质的功能都具有重要的意义.