摘要：Aquaculture,the most rapidly growing food production sector,has exhibited tremendous expansion over the past several decades and it currently accounts for more than half of the global fish production for human *** ensure sustainable aquaculture,it is necessary to breed farmed fish species(strains)with valuable economic traits,such as high production,high quality,disease resistance,and stress *** fish genetic breeding techniques largely rely on key processes including understanding the genetic basis of economically important traits to the generation and selection of favorable genotypes.

摘要：In the last decade,the unprecedented simplicity and flexibility of the CRISPR-Cas system has made it the dominant transformative tool in gene and genome ***,this democratized technology is both a boon and a bane,for which we have yet to understand the full potential to investigate and rewrite genomes(also named“genome biodesign”).Rapid CRISPR advances in a range of applications in basic research,agriculture,and clinical applications pose new risks and raise several biosecurity *** such a fast-moving field of research,we emphasize the importance of properly communicating the quality and accuracy of results and recommend new reporting requirements for results derived from next-generation genome engineering.

摘要：This article proves the existence of a hyper-precise global numerical meta-architecture unifying, structuring, binding and controlling the billion triplet codons constituting the sequence of single-stranded DNA of the entire human genome. Beyond the evolution and erratic mutations like transposons within the genome, it’s as if the memory of a fossil genome with multiple symmetries persists. This recalls the “intermingling” of information characterizing the fractal universe of chaos theory. The result leads to a balanced and perfect tuning between the masses of the two strands of the huge DNA molecule that constitute our genome. We show here how codon populations forming the single-stranded DNA sequences can constitute a critical approach to the understanding of junk DNA function. Then, we suggest revisiting certain methods published in our 2009 book “Codex Biogenesis”. In fact, we demonstrate here how the universal genetic code table is a powerful analytical filter to characterize single-stranded DNA sequences constituting chromosomes and genomes. We can then show that any genomic DNA sequence is featured by three numbers, which characterize it and its 64 codon populations with correlations greater than 99%. The number “1” is common to all sequences, expressing the second law of Chargaff. The other 2 numbers are related to each specific DNA sequence case characterizing life species. For example, the entire human genome is characterized by three remarkable numbers 1, 2, and Phi = 1.618 the golden ratio. Associated with each of these three numbers, we can match three axes of symmetry, then “imagine” a kind of hyperspace formed by these codon populations. Then we revisit the value (3-Phi)/2 which is probably universal and common to both the scale of quarks and atomic levels, balancing and tuning the whole human genome codon population. Finally, we demonstrate a new kind of duality between “form and substance” overlapping the whole human genome: we will show that—simult

摘要：根据抑制消减杂交(SSH)片段设计了两个特异性引物(GSP1和GSP2),并利用UniversalgenomeWalker技术,获得510bp的基因序列。blast比对结果表明该基因序列与玉米T胞质雄性不育恢复因子基因、反转录转座子反转录酶基因高度同源,可能为温敏核雄性不育相关基因。