摘要：The implementation of multiple enzymes for chemical production in a cell‐free scenario is an emerging field in *** enables the redesign and reconstitution of new enzymatic routes for producing chemicals that may be hard to obtain from natural *** the construction of a cell‐free multienzyme system is highly flexible and adaptable,it is challenging to make all enzymatic reactions act in ***,modular construction has been conceptual‐ized as an effective way to harmonize diverse enzymatic *** this review,we introduce the concept of a multienzyme module and exemplify representative modules found in *** then categorize recent developments of synthetic multienzyme modules into main‐reaction modules and auxiliary modules according to their roles in reaction *** highlight four main‐reaction mod‐ules that can perform carbon metabolism,carbon assimilation,protein glycosylation and nonribo‐somal peptide synthesis,and exemplify auxiliary modules used for energy supply,protection and reinforcement for main *** reactor‐level modularization of multienzyme catalysis is also discussed.