摘要：[Objective] The aim was to lay a foundation for research of contraceptive rodenticide with ldh-c4 as target protein. [ Method] EST sequence of ldh-c gene from black-lipped pika was cloned by PcR with degenerate primers; then the full length open reading frame （ORF） and 3＇UTR sequence were cloned by RAcE technique. [ Result] The full length cDNA was 1 498 bp containing an ORF of 996 bp and a 3＇UTR of 486 bp. The ORF encoded a polypeptide of 332 amino acids. The alignment of ldh-c gene ORF nucleotide sequences from different species showed that the gene was conserved even between large taxons. The phylogenic tree showed that black-lipped pika ldh-c was closer to prima- tes and artiodactyla than to rodents. [conclusion] cDNA sequence of ldh-c gene from black-lipped pika was cloned successfully.