摘要：根据二醇脱水酶与甘油脱水酶的激活因子基因序列同源性分析,设计简并引物从***基因组DNA中扩增出了假定的二醇脱水酶激活因子基因(gldG、gldH)全序列,补全了GenBank中该基因序列的未知部分,构建了表达质粒pSE-gldGH,以***21为宿主,进行诱导表达.表达产物的变性与非变性聚丙烯酰胺凝胶电泳分析表明:gldGH表达产生68ku、13ku两个蛋白质,它们经金属螯合亲和层析与凝胶过滤得到共纯化,纯化产物即假定的激活因子为分子质量约325ku的蛋白质聚合体,由这两个蛋白质以等摩尔量组成,因此假定的激活因子可能为α4β4亚基结构.以***二醇脱水酶为对象,进行激活实验,结果证实gldGH表达产物具备二醇脱水酶激活因子的功能.

摘要：对苯乳酸(PLA)高产菌株副干酪乳杆菌lactobacillus paracaseiW2的培养条件进行优化,以提高PLA合成能力。采用Plackett-Burman(PB)试验设计法,筛选出3个主要影响因子:葡萄糖、吐温-80、苯基丙酮酸(PPA)。通过最陡爬坡法和响应面分析方法(RSM)确定葡萄糖质量浓度19.5g/L、吐温-804mL/L、苯基丙酮酸质量浓度3g/L。在此优化条件下,lactobacillus paracaseiW2发酵合成苯乳酸的产量达到801mg/L,比优化前提高1.4倍。

摘要：本研究通过葡聚糖硫酸钠(DSS)诱导小鼠结肠炎模型,观察了lactobacillus plantarum YS-2(LP-YS2)对结肠炎抑制效果。将实验小鼠分为正常组、模型组、LP-YS2-L组、LP-YS2-H组和柳氮磺胺吡啶组,对除正常组外其余组小鼠诱导结肠炎,同时对LP-YS2-L组、LP-YS2-H组和柳氮磺胺吡啶组小鼠每日分别灌胃0.2 m L的1×108CFU/mL LP-YS2、1×10~9CFU/mL LP-YS2和20 mg/kg柳氮磺胺吡啶,持续5周。然后通过H&E切片观察小鼠结肠形态,通过内皮素(ET)、生长抑素(SS)、P物质(SP)、血管活性肠肽(VIP)、髓过氧化物酶(MPO)、谷胱甘肽(GSH)、丙二醛(MDA)、超氧化物歧化酶(SOD)、白介素-2(IL-2)、白介素-10(IL-10)试剂盒检测小鼠的血清和结肠组织;同时,采用荧光定量PCR技术对小鼠结肠组织神经元型一氧化氮合酶(nNOS)、内皮型一氧化氮合酶(eNOS)、诱导型一氧化氮合成酶(iNOS)、c-Kit、干细胞因子(SCF)、白介素-8(IL-8)、趋化因子受体2(CXCR2)的mRNA表达进行了检测,同时以药物柳氮磺胺吡啶为阳性对照比较LP-YS2的作用效果。实验结果显示相对于模型组,LP-YS2可以显著(p<0.05)增加结肠炎小鼠结肠长度和提高结肠质量/结肠长度的比值。对小鼠血清进行的检测表明LP-YS2能够显著(p<0.05)降低结肠炎小鼠(模型组)血清ET、SP、IL-10水平和显著(p<0.05)提高SS、VIP、IL-2水平。对小鼠结肠组织的检测显示LP-YS2可以显著(p<0.05)提高结肠炎小鼠(模型组)结肠中GSH含量、SOD活力和降低MPO活力、MDA含量;LP-YS2还能够显著(p<0.05)上调结肠炎小鼠(模型组)结肠中的n NOS、eNOS、c-Kit、SCF mRNA表达和显著(p<0.05)下调iNOS、IL-8、CXCR2表达。由此可以看出,LP-YS2具有结肠炎抑制效果。

摘要：A plant fermentation was carried out by Yeast and Lactobaccilli against fermented black turmeric, Kaempferia parviflora (FBT). These materials were proved by as safe in animal safety experiment. We tried to investigate changes of immune-competent cells that commonly utilized FBT, including after administration of immno-suppressed animals, the effects by FBT on the regulated effect on the cells were evaluated. Our results showed that FBT augmented the level of lymphocytes in number, while FBT regulated the level of granulocytes in both number and function. In our clinical study with 20 healthy volunteers, granulocyte and lymphocyte ratio suggesting their constitution as neutral in peripheral blood were increased significantly 30 days after the administration of FBT in rodents, and compromised host was prepared with cancer chemotherapeutic agent (Mytomycin-C). Our observations showed against intracellular parasite, and that FBT augmented intercellular pathogen through humoral immunity. We discussed the significance and mechanism of FBT on the level of leukocyte subsets in number and function that were considered to be potential indicators for the activation of the compromised host. We also proposed an idea that FBT exhibited tonic effects via activating complement components. The evidences were shown by immune-electrophoretic method. Moreover, we tried to access further to the anti-oxidative activities of this FBT. This modification brought to the significant lift up for antibody producing cells and anti-oxidative activity for phagocytic cells.

摘要：A plant material consisted by Family Poaceae was fermented by Yeast and Lactobaccilli (U-164). This material was proved by as safe in animal safety experiment for oral administration. In order to prove the effect of U-164 against physiological function, the animal and human trials were set up to look into mainly leukocyte functions. In animal experiment, anti-oxidative effect and antibody response in immune-compromised host and diabetes meritus were made up. For human use, peripheral lymphocyte in number and subset ratio were followed up to one month after administration. In order to understand its effect, human complement component analysis was made by immune-electrophoresis. Our results showed that U-164 augmented the level of lymphocytes, while U-164 down regulated the level of granulocytes. In our clinical study with 19 healthy volunteers, granulocyte and lymphocyte ratio was obtained as neutral in peripheral blood being increased significantly 30 days after the ingestion of U-164. In experimental animal study, the compromised host as well as normal animal was administered with cancer chemotherapeutic agent (Mytomycin-C). Our observations showed against antibody producing cell, this material recovered the antibody production in the host compromising the immure responsiveness. We also proposed an idea that U-164 exhibited tonic effects via activating complement components. Moreover, we tried to access further to the anti-oxidative activities of this U-164. This modification brought to the significant lifted up for anti-oxidative activity for phagocytic cell.

摘要：古井贡酒的酿造过程中,窖泥和酒醅中发现大量耐酵乳杆菌(lactobacillus acetotolerans),对该菌快速特异性检测非常重要。该文利用L. acetotolerans菌及大量lactobacillus类似菌种的全基因组序列与PRIMERBLAST方法,设计了几对L. acetotolerans特异性引物,其中2对经过系列验证具有较好的特异性,可结合QPCR技术对不同质量和不同发酵阶段的窖泥进行L. acetotolerans含量的快速检测。结果表明,古井贡酒窖泥中L. acetotolerans含量总体明显低于酒醅,老窖泥中L. acetotolerans含量总体明显低于新窖池窖泥;而酒醅中老厂区和新厂区L. acetotolerans含量平均来看都很高,但样本之间存在明显波动。所用L. acetotolerans特异性引物可对浓香型白酒发酵样本中L. acetotolerans的相对含量测定并辅助评估窖泥酒醅质量。

摘要：Given the increasing commercial and clinical relevance of probiotics, improving their stress tolerance profile and ability to overcome the physiochemical defences of the host is an important biological goal. Herein, I review the current state of the art in the design of engineered probiotic cultures, with a specific focus on their utility as therapeutics for the developing world; from the treatment of chronic and acute enteric infections, andtheir associated diarrhoeal complexes, to targeting HIV and application as novel mucosal vaccine delivery vehicles.