摘要：[Objective] The aim was to optimize Yanbian cow oocytes mature in vitro and cleavage system after nuclear transfer based on uniform design. [Method] Oocytes were recovered by aspiration method, and oocytes were matured in vitro （IVM） with different conditions, and then carried out nucleus transfer, fusion, activation and in vitro culture （IVC） of embryo. Effects of ovary storage temperature, maturation time and follicular diameter size on in vitro maturation and cleavage rates of cow oocytes were compared. [ Result] The best conditions of IVM of Yanbian cow oocytes was that： the oocytes of 8 mm diameter were matured in vitro for 24 hours when the ovaries were stored at 26℃ or 31 ℃. The best cleave conditions after nucleus transfer of oocytes was that： the oocytes of 6 mm or 8 mm diameter were cultured in vitro for 24 hours when the ovaries were stored at 26℃. [ Conclusion] The result has some reference to Yanbian cow and other cow breeding and population expanding propagation.

摘要：Purpose: Can Cortisol Releasing Hormone (CRH) levels in follicular fluid predict outcomes following assisted reproductive treatment (ART) cycles? Methods: Prospective cohort study of 50 women undergoing in vitro fertilisation (IVF)/intra-cytoplasmic sperm injection (ICSI) cycles over a two month study period. All patients were treated on the long stimulation protocol;follicular fluid was aspirated and pooled for each patient. The samples were processed appropriately and assayed using CRH radioimmunoassay (RIA). Results: This study confirmed that CRH was present in follicular fluid. The average level detected was 173 ± 9 pg/mL (mean ± standard error of mean [SEM]). The data suggests a positive correlation of CRH follicular fluid levels greater than 145 pg/mL with successful ART outcomes. Conclusion: The data indicates a positive correlation between ART outcomes and the presence of follicular fluid CRH levels greater than 145 pg/mL. The results should be interpreted with caution due to the small sample size and pooling of follicular fluid per patient. Furthermore, the pooling of follicular fluid is not representative of CRH levels in an individual follicle, and thus, mature oocyte. This study serves as a reminder to what has previously been hypothesised.

摘要：Based on wood structure of ancient buildings in China,the paper considers that Chinese ancient people have selected wood as the architectural material due to its characteristics and some *** the analysis of architectural features of traditional settlements,it has found that Chinese ancient people tend to draw on local materials for ***,it further analyzes the adaptability of Chinese wood structure,and the flexibility and complexity of its plane *** combining with Chinese's life value,the eternal significance contained in wood structure has been explained based on material and spiritual *** the perspective of natural selection,technological advancement,spatial advantage,spiritual value and aesthetic need,the paper tries to find causes for wood structure as architectural materials,aiming to objectively treat the effort of Chinese ancient people made in the progress history of human civilization.

摘要：一开始,我们没有做过太多的管理。那是一段不同寻常的扩展期,特别是在中东与中国办事处。甚至在欧洲,我们也有项目遍布西班牙、意大利与法国。西班牙的每个城市都有项目,在迪拜与卡塔尔等国家还有大型项目。我们当时还是很年轻的事务所,但是盈利上升很快。挣得多,开销也不小。