摘要：Drug resistance is one of the major obstacles in the drug therapy of *** in this area in pre-clinical research have focused on developing novel platforms to evaluate and decrease drug *** this paper,inspired by the structure of hives where swarms live and breed,we propose porous hydrogel arrays with a uniform pore structure for the generation of hepatoma cell spheroids and the investigation of drug *** porous hydrogel arrays were fabricated using polyeth-ylene glycol diacrylate(PEGDA)hydrogel to negatively replicate a well-designed *** from the elaborate processing of the template,the prepared porous hydrogel arrays possessed a uniform pore *** to their anti-adhesion properties and the excellent biocompatibility of the PEGDA hydrogel,the hepatoma cells could form well-defined and uni-form hepatoma cell spheroids in the porous hydrogel *** found that the resistant hepatoma cell spheroids showed more significant Lenvatinib resistance and a migratory phenotype compared with a two-dimensional(2D)cell culture,which reveals the reason for the failure of most 2D cell-selected drugs for in vivo *** features give such porous hydrogel arrays promising application prospects in the investigation of tumor cell spheroid culture and in vitro drug resistance.

摘要：A novel method was proposed to design the structure of a bone tissue engineering scafold based on triply periodic minimal *** this method,reverse engineering software was used to reconstruct the surface from point cloud *** method overcomes the limitations of commercially available software packages that prevent them from generating models with complex surfaces used for bone tissue engineering ***,the fluid feld of the scafolds was simulated through a numerical method based on fnite volume and the cell proliferation performance was evaluated via an in vitro *** cell proliferation and the mass flow evaluated in a bioreactor further verifed the flow feld simulated using computational fluid *** result of this study illustrates that the pressure value drops rapidly from 0.103 Pa to 0.011 Pa in the y-axis direction and the mass flow is unevenly distributed in the *** mass flow in the side outlets is observed to be approximately 24.3 times higher thanthe ***,although the mean value of wall shear stress is signifcantly more than 0.05 Pa,there is stil a large area with a suitable shear stress below 0.05 Pa where most cells can proliferate *** result shows that th inlet velocity 0.0075 m/s is suitable for cell proliferation in the *** study provides an insight into the design analysis,and in vitro experiment of a bone tissue engineering scafold.

摘要：采用电子束熔化(EBM)成形工艺,制造具有可控多孔结构的Ti6Al4V植入体,分析测试其微观孔隙结构特征、孔隙率以及力学性能。扫描电镜观测结果表明,所制备的钛合金植入体孔隙结构特征与设计结构相符合,证明EBM技术能够实现钛合金植入体孔隙结构的控制;测得多孔植入体的孔隙率为60.1%,相应的抗压强度为163MPa,弹性模量为14GPa,与人体骨组织弹性模量相近。利用改进的碱热处理方法进行表面改性,并浸泡在模拟体液中以诱导磷灰石的形成。体外细胞培养试验结果表明,培养7d后成骨细胞在改性的试件表面大量粘附、生长、增殖。