摘要：Background: Diagnostic microbial isolates of bio-safety levels 3 and 4 are difficult to handle in medical field camps under military deployment settings. International transport of such isolates is challenging due to restrictions by the International Air Transport Association. An alternative option might be inactivation and sequencing of the pathogen at the deployment site with subsequent sequence-based revitalization in well-equipped laboratories in the home country for further scientific assessment. Methods: A literature review was written based on a Pub Med search. Results: First described for poliovirus in 2002, de novo synthesis of pathogens based on their sequence information has become a well-established procedure in science. Successful syntheses have been demonstrated for both viruses and prokaryotes. However, the technology is not yet available for routine diagnostic purposes. Conclusions: Due to the potential utility of diagnostic sequencing and sequence-based de novo synthesis of pathogens, it seems worthwhile to establish the technology for diagnostic purposes over the intermediate term. This is particularly true for resource-restricted deployment settings, where safe handling of harmful pathogens cannot always be guaranteed.

摘要：[目的]建立荷斯坦牛凝血因子Ⅺ的PCR检测方法。[方法]参照GenBank上登录的牛FⅪ(FactorⅪ)基因序列,设计并合成1对特异性引物,应用PCR方法对河南省周边牛场576头荷斯坦牛进行检测,并对所有个体扩增出的DNA片段测序。[结果]PCR检测的576头奶牛中有2头FⅪ携带者和1头FⅪ缺陷症患者,与DNA序列测定结果一致,等位基因突变率为0.3%,携带率为0.3%,患病率为0.2%。[结论]建立了荷斯坦牛FⅪ的PCR检测方法,该方法具有简便、快速、特异性好等优点,适合荷斯坦牛的大规模检测。

摘要：目的MHCⅠ类链相关基因B（MICB）是非经典的HLAⅠ类分子,可与NKG2D相互作用调节NK细胞的活性。本研究旨在建立MICB基因2～6外显子的聚合酶链反应-序列测序方法（PCR-SBT）,并分析汉族人群MICB在高分辨基因分型水平上的分布特征。方法根据GenBank数据库MICB基因有关序列（ID NC000006.11）,采用Primer3input计算机软件辅助设计引物,利用聚合酶链反应扩增MICB基因2～6外显子序列片段,扩增片段采用双酶切纯化后,按BigDye terminator v3.1sequencing Kit试剂盒操作进行测序反应,利用Assign3.5SBT分析软件指定等位基因型。

摘要：目的人类粒细胞特异性抗原在不同人群中表现出高度的多态性,输注后由于个体抗原的差异,可以刺激不同的机体产生免疫反应,从而引发某些不良反应。本研究拟建立1种基于DNA扩增和测序分型(PCR sequencing-based typ-ing,PCR-SBT)的HNA-1、HNA-3到HNA-5和HNA-2a(G/C)抗原系统的基因分型技术。方法选择参与本单位献血人员的随机标本400例,采用DNA抽提试剂盒,抽提标本基因组DNA,严格按试剂说明书进行操作,参照数据库中人类HNA-1～HNA-5基因序列,采用计算机软件设计引物,并在引物5’端添加M13序列,5对引物分别扩增HNA-1～HNA-5基因序列,