摘要：Of late, the relevance of silk in a myriad of material science and biotechnological realms has been realized, as attested by the incessantly clambering number of reports and patents in the scienti fic repositories. The write-up is geared off with a scrutiny into the pertinence of the basic nano-structural features of silk, christened as the ‘queen of textile’ for exemplary bioengi- neering applications including designing and fabrication of devices for micro fluidics, opto fluidics, chemo/bio sensing, etc. Then, the major thrust of this short review is directed towards comprehending the prospects of using silk-based biomaterials (e.g. sca ffolds, electrospun membranes, films, hydrogels, bioinks) for tissue engineering and regenerative medicine as well as targeted delivery of various biomolecular cargoes/therapeutic agents, etc., as vouched by few avant-garde endeavours of the recent years. The write-up is entwined with a discussion on the various factors that could plausibly hinder the realization of silk as the next-generation nanobiomaterial, suggestions for some approaches to dodge and deal with the practical snags and what lies ahead!

摘要：The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered sca ffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCP) bottom layer were developed. Furthermore, a comparative analysis between two types of sca ffolds pro- duced using di fferent SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced sca ffolds presented homogeneous porosity in both layers and no di fferences were observed when comparing both sca ffolds. A decrease in terms of mechanical performance of the sca ffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 MPa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 MPa. By its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 MPa and dynamical mechanical properties ranging from 1.03 ± 0.08 MPa to 1.56 ± 0.13 MPa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hMCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCP and SF16/TCP) of the hierarchical sca ffolds Hi8 and Hi16, respectively. Both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar Blue assay and DNA quanti fication analysis. Subcutaneous implantation in mice revealed weak in flammation and sca ffold’s integrity. The hierarchical indirect printed SF sca ffolds can be promising candidate for meniscus TE sca ffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patie

摘要：对于古代行走在丝绸之路上的商队来说,由于路途面临恶劣的自然环境,加之古代丝绸之路上经常会有盗贼出没,因此他们一般会结伴而行,由商队首领率领,以商队的形式奔波于丝绸之路上。丝绸之路商队所经营的商品,当以奢侈品为主,具有体积小,便于携带而价格昂贵、利润高的特点,这主要是由于在欧亚内陆进行长途贩运的难度太大。

摘要：流量统计与异常检测技术具有广泛的应用前景,从一个实际的应用项目出发,针对Netflow流数据的生成、输出、采集、统计、检测等几个方面进行了大量深入的研究,并对统计结果的输出方式、监测算法的参数优化提出了改进。在此基础上,设计并实现了一个基于Linux平台的流量统计与异常检测系统。

摘要：一个拥有哲学、数学、工业系统工程、公共行政管理等多个学位的学者 一个在美国软件公司 开始就做系统设计的软件工程师 一个让别人损失了65万美元，还依然被人委以重任的创业者 一个矢志不渝，57岁依然热情澎湃要打败微软的软件豪侠