摘要：To explore the feasibility of repairing clinical cutaneous deficiency, autogenic bone marrow mesen-chymal stem cells (BMSCs) were isolated and differentiated into epidermal cells and fibroblasts in vitro supplemented with different inducing factors and biomaterials to construct functional tissue- engineered skin. The results showed that after 72 h induction, BMSCs displayed morphologic changes such as typical epidermal cell arrangement, from spindle shape to round or oval; tonofibrils, melano-somes and keratohyaline granules were observed under a transmission electronic microscope. The differentiated cells expressed epidermal stem cell surface marker CK19 (59.66% ± 4.2%) and epidermal cells differentiation marker CK10. In addition, the induced epidermal cells acquired the anti-radiation capacity featured by lowered apoptosis following exposure to UVB. On the other hand, the collagen microfibrils deposition was noticed under a transmission electronic microscope after differentiating into dermis fibroblasts; RT-PCR identified collagen type I mRNA expression in differentiated cells; radioimmunoassay detected the secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8) (up to 115.06 pg/mL and 0.84 ng/mL, respectively). Further in vivo implanting BMSCs with scaffold material short-ened skin wound repair significantly. In one word, autogenic BMSCs have the potential to differentiate into epidermal cells and fibroblasts in vitro, and show clinical feasibility acting as epidermis-like and dermis-like seed cells in skin engineering.

摘要：随着我国课程改革的不断深入,近年来各种新的教学理念和方法层出不穷。stem是一种融合了科学、技术、工程和数学等多领域知识的跨学科教育理念,将其应用于初中生物实验教学,可以发展学生的生物素养,激发学生的学习兴趣。本文从当前初中生物实验教学存在的问题出发,分别从甄选活动主题、创新教学方法、优化作业设计和重视实验结果的评价交流四个方面,提出了基于stem教育的初中生物实验设计策略,希望促进初中生物实验教学质量的提升。

摘要：stem(科学、技术、工程和数学)是一种综合性的教育理念,旨在培养学生的创新思维和解决问题能力。物理学作为stem教育的重要组成部分,其课程内容的整合与设计对于培养学生的科学素养和创新能力具有重要意义。文章探讨基于stem教育理念的中学物理课程内容的整合,为stem教育理念的落实提供有益的借鉴。

摘要：项目预设的理论层面目标预期围绕张江高科园区这一特定的科教课程开发背景，我们锁定了两大课程开发的借鉴指标体系，并藉此确定了开放课程开发指南。这就是PISA和stem。前者自2009年正式引入上海后，就成为了检测学业素养的一个重要参照体系；而后者所圈定的（科学、技术、工程、数学）课程体系恰好契合张江科教的地域文化和学校特色定位。这就为探索社会资源的开源性素养课程开发基本范式，以及资源课程与知识课程交融的可行性研究目标预设，提供了前瞻性的思考高度，也初步界定出基于社会资源的素养课程开发基准，凸显了开放性的课程价值及课程游学特性。

摘要：Human placenta-derived stem cells (hPDSCs) were isolated by trypsinization and further induced into cartilage cells in *** engineered cartilage was constructed by combining hPDSCs with collagen sponge and the cartilage formation was observed by implantation into nude *** showed that hPDSCs featured mesenchymal stem cells and maintained proliferation in vitro for over 30 passages while remaining *** results indicated that hPDSCs have the potential to differentiate into functional cartilage cells in vitro when combined with collagen sponge,which provided experimental evidence for prospective clinical application.

摘要：Recent regenerative medicine and tissue engineering strategies(using cells, scaffolds, medical devices and gene therapy) have led to fascinating progress of translation of basic research towards clinical applications. In the past decade, great deal of research has focused on developing various three dimensional(3D) organs, such as bone, skin, liver, kidney and ear,using such strategies in order to replace or regenerate damaged organs for the purpose of maintaining or restoring organs' functions that may have been lost due to aging, accident or disease. The surface properties of a material or a device are key aspects in determining the success of the implant in biomedicine, as the majority of biological reactions in human body occur on surfaces or interfaces. Furthermore, it has been established in the literature that cell adhesion and proliferation are, to a great extent, influenced by the micro- and nanosurface characteristics of biomaterials and devices. In addition, it has been shown that the functions of stem cells, mesenchymal stem cells in particular, could be regulated through physical interaction with specific nanotopographical cues. Therefore, guided stem cell proliferation, differentiation and function are of great importance in the regeneration of 3D tissues and organs using tissue engineering strategies. This review will provide an update on the impact of nanotopography on mesenchymal stem cells for the purpose of developing laboratory-based 3D organs and tissues, as well as the most recent research and case studies on this topic.

摘要：stem cells are capable of long-term self-renewal and differentiation into specialised cell types, making them an ideal candidate for a cell source for regenerative medicine. The control of stem cell fate has become a major area of interest in the field of regenerative medicine and therapeutic intervention. Conventional methods of chemically inducing stem cells into specific lineages is being challenged by the advances in biomaterial technology, with evidence highlighting that material properties are capable of driving stem cell fate. Materials are being designed to mimic the clues stem cells receive in their in vivo stem cell niche including topographical and chemical instructions. Nanotopographical clues that mimic the extracellular matrix(ECM) in vivo have shown to regulate stem cell differentiation. The delivery of ECM components on biomaterials in the form of short peptides sequences has also proved successful in directing stem cell lineage. Growth factors responsible for controlling stem cell fate in vivo have also been delivered via biomaterials to provide clues to determine stem cell differentiation. An alternative approach to guide stem cells fate is to provide genetic clues including delivering DNA plasmids and small interfering RNAs via scaffolds. This review, aims to provide an overview of the topographical, chemical and molecular clues that biomaterials can provide to guide stem cell fate. The promising features and challenges of such approaches will be highlighted, to provide directions for future advancements in this exciting area of stem cell translation for regenerative medicine.

摘要：21世纪迅速变化的知识和信息社会正在迫使stem教育转向STEAM教育,社会期望艺术的深度融合能够平衡仅仅围绕科学技术进行教育的诸多弊端。然而,STEAM教育目前面临着艺术如何对接以及如何融入stem教育这一巨大挑战。从stem教育走向STEAM教育的过程是艰辛的,化解stem教育弊端及STEAM教育挑战需要从艺术角色本身进行深度分析。事实上,STEAM教育中的"A"包含精致艺术等五种内容,传递着大艺术视角等六维理念,发挥着提升创造力等四度功能。Hetland等人以工作室思维为艺术和stem教育的成功对接提供了模型,Bresler则为艺术融入stem教育提供了必要的支持和结构。STEAM教育运动将是未来很长一段时期教育领域关注的热点问题,助推STEAM教育深入发展的建议有三个方面,一是重视跨学科大概念,二是强调基于艺术的实践,三是挖掘适切教育资源。

摘要：知识爆炸时代，信息加速冲击人们的大脑。人们对大段的文字信息渐渐缺乏耐心，动漫、卡通、信息图、图文广告、影视作品充斥我们的视觉，成为我们接受信息的主角。同样，当我们用大段文字讲解观点，也会让听众昏昏欲睡。为有效传达信息，图文并茂是必然之选，我们已经进入了读图时代，面对这个时代的能力要求，我们正在实践着一种课堂教学改变的模型--可视化沟通，以及由此派生出的新课程《可视化沟通》。

摘要：stem教育因其跨学科、联结真实世界、育“全人”等特性,与当代教育之“全人”发展目标高度统一,逐渐为一线教师认同。游戏化对于stem学习具有天然优势,运用游戏化方式开展stem学习对于学生的全面发展具有重要价值。本研究采用基于设计的方法,依据“设计-制作-游戏”学习方法,采取stem与游戏化融合策略,提出stem游戏化学习设计框架DMP,经由为期三个月三轮的stem游戏化学习活动实践,迭代检验与修正DMP框架,最终形成包括“两驱三环七步”的DMP设计框架。实践表明,经过迭代修正的DMP设计框架初见成效,学生对stem游戏化学习活动过程满意,跨学科知识建构、问题解决与高阶思维等得到展显著发展,达成预期目标。本研究构建的DMP设计框架可以供stem游戏化学习活动设计与实践参考。